MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1245_Other_P1_B10.mgf |
| Database | : | S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues) |
| Timestamp | : | 24 Jun 2025 at 21:21:38 GMT |
Not what you expected? Try 
the select summary.
| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,780 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.
1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS3_YEAST | 66 | 40S ribosomal protein S3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RPS3 PE=1 SV=5 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q9FD70_ENTFL | 66 | Acetyl-CoA acetyltransferase/HMG-CoA reductase OS=Enterococcus faecalis GN=mvaE PE=3 SV=1 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PGK_YEAST | 62 | Phosphoglycerate kinase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PGK1 PE=1 SV=2 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PMG1_YEAST | 43 | Phosphoglycerate mutase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GPM1 PE=1 SV=3 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS19A_YEAST | 36 | 40S ribosomal protein S19-A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RPS19A PE=1 SV=2 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EF1A_YEAST | 32 | Elongation factor 1-alpha OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TEF1 PE=1 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ERR3_YEAST | 25 | Enolase-related protein 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ERR3 PE=3 SV=2 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 7.1 | ERR3_YEAST |
25 | 47682 | 3 (1) | 2 (1) | 0.07 | |
| Enolase-related protein 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ERR3 PE=3 SV=2 | |||||||
| 4 samesets of ERR3_YEAST | |||||||
| ENO1_YEAST |
25 | 46844 | 1 (1) | 1 (1) | 0.07 | ||
| Enolase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ENO1 PE=1 SV=3 | |||||||
| ERR1_YEAST |
25 | 47697 | 1 (1) | 1 (1) | 0.07 | ||
| Enolase-related protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ERR1 PE=3 SV=1 | |||||||
| ENO2_YEAST |
25 | 46942 | 1 (1) | 1 (1) | 0.07 | ||
| Enolase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ENO2 PE=1 SV=2 | |||||||
| ERR2_YEAST |
25 | 47697 | 1 (1) | 1 (1) | 0.07 | ||
| Enolase-related protein 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ERR2 PE=3 SV=1 | |||||||
3 peptide matches (3 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1166 |
400.6963 | 799.3780 | 799.3752 | 3.57 | 0 | 25 | 0.0093 | 1Score > 17 indicates identity |
U | K.YDLNFK.E + Deamidated (NQ) | |
| 2464 |
576.7684 | 1151.5222 | 1151.5393 | -14.8 | 0 | 3 | 1 | 1Score > 21 indicates identity Score > 15 indicates homology |
U | K.AINICGYEGR.V | |
D:\Xcalibur\Data\Yan\PRT1245 redo\PRT1245_Other_P1_B10.rawScore > 21 indicates identity Score > 20 indicates homology |
|||||||||||
| 2.71 | 1 | 11 | 0.46 | 1 | KNVEDDGYGR | ||||||
| 2466 |
1152.5311 | 1151.5238 | 1151.5393 | -13.5 | 0 | 7 | 1.1 | 2Score > 21 indicates identity Score > 20 indicates homology |
U | K.AINICGYEGR.V | |
| -16.4 | 0 | 7 | 1.1 | 3 | LTNSLMMNGR + Oxidation (M) | ||||||
| -16.4 | 0 | 7 | 1.1 | 3 | LTNSLMMNGR + Oxidation (M) | ||||||
| -15.6 | 1 | 3 | 2.6 | 5 | QESGKSTSTAR + Deamidated (NQ) | ||||||
| -0.23 | 0 | 2 | 3.3 | 6 | GSSPSLCSTTR | ||||||
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ELP4_YEAST | 18 | Elongator complex protein 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ELP4 PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SERC_YEAST | 15 | Phosphoserine aminotransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SER1 PE=1 SV=1 |
Not what you expected? Try the select summary.
Mascot: http://www.matrixscience.com/