MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 801–900 (out of 4700)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
115 309.2079 308.2006
116 309.2079 308.2006
117 309.2082 308.2009
118 309.2085 308.2012
119 309.2088 308.2015
120 309.2091 308.2018
121 310.1917 309.1844
122 310.1919 309.1846
123 311.1675 310.1602
124 311.1678 310.1605
125 311.1682 310.1609
126 311.1683 310.1610
127 311.1688 310.1615
128 311.2050 310.1977
129 311.2061 310.1988
130 311.2061 310.1988
131 311.2062 310.1989
132 311.2065 310.1992
133 311.2066 310.1993
134 311.2236 310.2163
135 311.2239 310.2166
136 311.2240 310.2167
137 311.2241 310.2168
138 311.2245 310.2172
139 312.0680 311.0607
140 312.1329 311.1256
141 312.1329 311.1256
142 312.1330 311.1257
143 312.2086 311.2013
144 313.1839 312.1766
145 313.1839 312.1766
146 313.1844 312.1771
147 313.1848 312.1775
148 313.1856 312.1783
149 313.1862 312.1789
150 313.1864 312.1791
151 313.1867 312.1794
152 313.2210 312.2137
153 313.2216 312.2143
154 313.2385 312.2312
155 315.2015 314.1942
156 316.1914 315.1841
157 316.1914 315.1841
158 316.2242 315.2169
159 317.2110 316.2037
160 318.1303 317.1230
161 318.1823 317.1750
162 319.1900 318.1827
163 319.1902 318.1829
164 319.1904 318.1831
165 319.1907 318.1834
166 319.1909 318.1836
167 319.1909 318.1836
168 319.1910 318.1837
169 319.1913 318.1840
170 319.2263 318.2190
171 319.2270 318.2197
172 319.2271 318.2198
173 319.2271 318.2198
174 319.2272 318.2199
175 319.2274 318.2201
176 321.1700 320.1627
177 321.1701 320.1628
178 321.1701 320.1628
179 321.1702 320.1629
180 321.1702 320.1629
181 321.1703 320.1630
182 321.1703 320.1630
183 321.1709 320.1636
184 321.2054 320.1981
185 321.2428 320.2355
186 321.2429 320.2356
187 321.2430 320.2357
188 321.2622 320.2549
189 322.2456 321.2383
190 323.1678 322.1605
191 323.1679 322.1606
192 323.1681 322.1608
193 323.1685 322.1612
194 323.1685 322.1612
195 323.1696 322.1623
196 323.1703 322.1630
197 323.1855 322.1782
198 323.1856 322.1783
199 323.1858 322.1785
200 323.1859 322.1786
201 323.1859 322.1786
202 323.1860 322.1787
203 323.1863 322.1790
204 323.1865 322.1792
205 323.1867 322.1794
206 323.1868 322.1795
207 323.1873 322.1800
208 323.1885 322.1812
209 323.2031 322.1958
210 323.2055 322.1982
211 323.2059 322.1986
212 323.2060 322.1987
213 323.2060 322.1987
214 325.1822 324.1749
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