MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 701–800 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
15 301.2214 300.2141
16 301.8908 300.8835
17 301.8909 300.8836
18 301.8911 300.8838
19 301.8912 300.8839
20 302.1714 301.1641
21 303.8853 302.8780
22 303.8861 302.8788
23 303.8864 302.8791
24 303.8864 302.8791
25 303.8864 302.8791
26 303.8865 302.8792
27 303.8866 302.8793
28 303.8867 302.8794
29 303.8867 302.8794
30 303.8870 302.8797
31 303.8870 302.8797
32 303.8872 302.8799
33 303.8873 302.8800
34 304.1664 303.1591
35 305.1734 304.1661
36 305.1747 304.1674
37 305.1749 304.1676
38 305.1750 304.1677
39 305.1750 304.1677
40 305.1750 304.1677
41 305.1751 304.1678
42 305.1755 304.1682
43 305.1755 304.1682
44 305.1758 304.1685
45 305.1758 304.1685
46 305.1759 304.1686
47 305.1763 304.1690
48 305.1945 304.1872
49 305.2104 304.2031
50 305.2108 304.2035
51 307.1542 306.1469
52 307.1545 306.1472
53 307.1545 306.1472
54 307.1550 306.1477
55 307.1898 306.1825
56 307.1908 306.1835
57 307.1909 306.1836
58 307.1909 306.1836
59 307.1910 306.1837
60 307.1910 306.1837
61 307.1913 306.1840
62 307.1916 306.1843
63 307.1918 306.1845
64 307.1919 306.1846
65 307.1922 306.1849
66 307.1944 306.1871
67 307.2101 306.2028
68 308.0897 307.0824
69 308.0899 307.0826
70 308.0899 307.0826
71 308.0900 307.0827
72 308.0905 307.0832
73 308.0906 307.0833
74 308.0907 307.0834
75 308.0909 307.0836
76 308.0909 307.0836
77 308.0911 307.0838
78 308.0913 307.0840
79 308.0914 307.0841
80 308.0918 307.0845
81 308.0920 307.0847
82 308.0921 307.0848
83 308.0921 307.0848
84 308.0922 307.0849
85 308.0922 307.0849
86 308.0923 307.0850
87 308.0925 307.0852
88 308.0925 307.0852
89 308.0926 307.0853
90 308.0926 307.0853
91 308.0927 307.0854
92 308.0928 307.0855
93 308.0928 307.0855
94 308.0928 307.0855
95 308.0929 307.0856
96 308.0929 307.0856
97 308.0930 307.0857
98 308.0930 307.0857
99 308.0930 307.0857
100 309.1519 308.1446
101 309.1520 308.1447
102 309.1697 308.1624
103 309.1703 308.1630
104 309.1704 308.1631
105 309.1710 308.1637
106 309.1889 308.1816
107 309.1891 308.1818
108 309.1894 308.1821
109 309.1902 308.1829
110 309.1902 308.1829
111 309.1905 308.1832
112 309.2059 308.1986
113 309.2071 308.1998
114 309.2072 308.1999
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