MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 501–600 (out of 4700)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4221 883.1387 2646.3943 2646.4317 -14.2 0 1 3.4 +1Score > 19 indicates identity VVMAIVWYAVQAWLGATPVALMLK + Deamidated (NQ); Oxidation (M)
1360 397.6882 793.3618 793.3640 -2.71 0 1 0.99 +1Score > 21 indicates identity
Score > 14 indicates homology
MAISDGGK + Oxidation (M)
2714 621.3268 1240.6390 1240.6550 -12.9 0 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
ALPNIENDIIK + 2 Deamidated (NQ)
2775 657.8084 1313.6022 1313.6186 -12.5 1 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
FRMNFEQGLR + Deamidated (NQ); Oxidation (M)
3665 1005.4990 2008.9834 2008.9751 4.18 0 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
VAPTKPMYCASFQLPANV + Oxidation (M)
2173 533.2672 1064.5198 1064.5390 -17.9 0 1 1 +1Score > 23 indicates identity
Score > 14 indicates homology
QITYNSIPK + 2 Deamidated (NQ)
2960 740.3579 1478.7012 1478.7286 -18.5 1 1 0.84 +1Score > 22 indicates identity
Score > 13 indicates homology
TSMSIIDDENVKK
2666 601.7927 1201.5708 1201.5761 -4.37 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
ANTPSSMHKTK + Deamidated (NQ)
2762 649.8251 1297.6356 1297.6302 4.18 1 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
ANNSLNNFKFK + 2 Deamidated (NQ)
3358 879.9225 1757.8304 1757.8328 -1.32 1 1 0.86 +1Score > 22 indicates identity
Score > 13 indicates homology
MQLQGEMSASAAKVYK + Deamidated (NQ); Oxidation (M)
1502 860.4210 859.4137 859.4188 -5.90 0 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
WADEALR
3221 552.9156 1655.7250 1655.7065 11.1 1 1 3.3 +1Score > 19 indicates identity RNVANTTNMNMNLM + Deamidated (NQ); 2 Oxidation (M)
1501 859.4873 858.4800 858.4811 -1.21 1 1 1 +1Score > 23 indicates identity
Score > 14 indicates homology
VDQELKK
1601 903.5104 902.5031 902.5073 -4.60 1 1 0.89 +1Score > 22 indicates identity
Score > 13 indicates homology
GELETKVK
2656 1195.5641 1194.5568 1194.5373 16.4 0 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
QVSASMGQVCK + Deamidated (NQ)
1853 982.5602 981.5529 981.5607 -7.94 0 1 2.5 +1Score > 18 indicates identity LLGAQPDIR
2657 598.3218 1194.6290 1194.6357 -5.54 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
LQDAIVDHKR + Deamidated (NQ)
3632 998.4750 1994.9354 1994.9619 -13.3 0 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
GIVDIYSNDVMIIDNNGK + Oxidation (M)
1384 403.2285 804.4424 804.4341 10.4 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
TISDKNK
3040 765.9080 1529.8014 1529.7937 5.06 0 1 0.96 +1Score > 23 indicates identity
Score > 13 indicates homology
ENQVVVIIGETGSGK + Deamidated (NQ)
3911 721.7194 2162.1364 2162.1041 14.9 1 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
DLDAPRPPLPQPMKQEVSK + Deamidated (NQ); Oxidation (M)
4216 873.4280 2617.2622 2617.2217 15.5 1 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
LSNVNISMNQENINNDTFLYKK + 3 Deamidated (NQ); Oxidation (M)
3094 785.8840 1569.7534 1569.7634 -6.37 0 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
KPEVVENEDVQQR + Deamidated (NQ)
4078 777.3752 2329.1038 2329.1260 -9.53 0 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
MSDNLLSLENPVVPSHYELR + Deamidated (NQ); Oxidation (M)
2087 1045.5029 1044.4956 1044.5087 -12.5 1 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
KDGEENKPK + Deamidated (NQ)
3111 792.8883 1583.7620 1583.7903 -17.9 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
NLGDAVPEVRDATAR + Deamidated (NQ)
2948 733.3454 1464.6762 1464.6919 -10.7 1 1 0.87 +1Score > 21 indicates identity
Score > 13 indicates homology
ILDLDPRTEAYC
3084 781.9004 1561.7862 1561.8100 -15.2 1 1 0.97 +1Score > 23 indicates identity
Score > 13 indicates homology
DINDININFAAKSK
1377 802.3975 801.3902 801.3994 -11.4 1 1 3.6 +1Score > 19 indicates identity HYGNRR
1998 511.7451 1021.4756 1021.4577 17.6 1 1 0.94 +1Score > 22 indicates identity
Score > 13 indicates homology
RGSGPYDDR
3872 712.3509 2134.0309 2134.0523 -10.1 1 1 1 +1Score > 23 indicates identity
Score > 14 indicates homology
WGPAAAIEFEYDPWNKLK
3679 1011.4586 2020.9026 2020.9082 -2.73 0 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
LNPDGTMAIDEETMVVDR + Oxidation (M)
4343 956.4891 2866.4455 2866.4521 -2.31 0 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
SNGMLDLLLQSDGSLIYSLNISQLLR + 4 Deamidated (NQ)
4681 1118.5420 5587.6736 5587.6592 2.59 1 1 2 +1Score > 17 indicates identity NLSETSSEFGFPNLIGFPTSIWDESLYKAWSQIVCSLIPNMSNHQSNLK + 2 Deamidated (NQ)
2660 600.3022 1198.5898 1198.5838 5.02 0 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
MGQLLYSMTR
3854 707.9954 2120.9644 2120.9902 -12.2 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
ANYGKNASIEYPPNPDELK + 2 Deamidated (NQ)
1999 1022.4968 1021.4895 1021.4941 -4.46 1 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
YGQNDRLR + Deamidated (NQ)
4349 961.4862 2881.4368 2881.4167 6.95 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
GLIIQFIDQAQTTFAQMQRQLDGEK + 3 Deamidated (NQ)
3180 407.9470 1627.7589 1627.7399 11.6 0 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
TQGYTVADCTAEAIK + Deamidated (NQ)
1851 491.7278 981.4410 981.4226 18.8 0 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
QGNEQMFK + Deamidated (NQ)
1436 833.4109 832.4036 832.4151 -13.8 1 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
NRTGTQR + Deamidated (NQ)
3138 400.9388 1599.7261 1599.7488 -14.2 0 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
NNVSQNINQSLPNR + 3 Deamidated (NQ)
4122 599.8105 2395.2129 2395.1695 18.1 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
SINEVFRFQQLLVHEYNEK + 3 Deamidated (NQ)
892 472.2443 471.2370 471.2441 -15.1 0 1 1.5 +1Score > 15 indicates identity GNPGK
1588 898.3903 897.3830 897.3716 12.8 0 1 2.4 +1Score > 17 indicates identity ADSGYEEK
2117 525.2931 1048.5716 1048.5553 15.6 0 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
LESFNLAVR + Deamidated (NQ)
2740 636.3233 1270.6320 1270.6452 -10.3 0 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
ALANTRPMEPR + Oxidation (M)
2883 714.8177 1427.6208 1427.6385 -12.3 0 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
MSVQESITQSMR + 2 Oxidation (M)
4043 768.3829 2302.1269 2302.1515 -10.7 0 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
LSMESPHVNVFSILTDLDIR + Deamidated (NQ); Oxidation (M)
3270 847.3845 1692.7544 1692.7817 -16.1 0 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
DTYMLLFGNQAYNK + Oxidation (M)
1155 678.3231 677.3158 677.3166 -1.20 0 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
NEMIR + Oxidation (M)
1828 488.2698 974.5250 974.5298 -4.83 1 1 0.89 +1Score > 22 indicates identity
Score > 13 indicates homology
RDVVYPAR
2083 1043.5104 1042.5031 1042.5005 2.53 0 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
GITTETMYK
1361 795.4126 794.4053 794.4188 -16.9 0 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
GLFHGHK
3119 794.9091 1587.8036 1587.8256 -13.8 1 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
AAQNPLADIQVYKR + 2 Deamidated (NQ)
4044 768.3962 2302.1668 2302.1993 -14.1 0 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
VALILFMQAIFAFENMGLIK + 2 Deamidated (NQ); 2 Oxidation (M)
2940 730.3890 1458.7634 1458.7905 -18.5 1 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
MTVVVLEKNPWK + Oxidation (M)
3006 754.9100 1507.8054 1507.7769 18.9 1 1 0.91 +1Score > 20 indicates identity
Score > 13 indicates homology
NATKELTFNLLNK + 3 Deamidated (NQ)
3080 781.8938 1561.7730 1561.7592 8.85 0 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
IICPSVESISNGMR
2756 645.3633 1288.7120 1288.6888 18.1 1 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
KVNVFNVNNNK
2770 655.3471 1308.6796 1308.6674 9.38 0 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
HDQTTGALQLPK + Deamidated (NQ)
3085 781.9028 1561.7910 1561.8100 -12.1 1 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
DINDININFAAKSK
1572 890.4423 889.4350 889.4392 -4.72 0 1 0.97 +1Score > 23 indicates identity
Score > 13 indicates homology
SNIANEIK + 2 Deamidated (NQ)
1608 453.2423 904.4700 904.4614 9.58 1 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
QGNKSTIR + 2 Deamidated (NQ)
3015 757.9141 1513.8136 1513.8075 4.07 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
KPTMIKLQFHNR + 2 Deamidated (NQ)
2985 749.3754 1496.7362 1496.7147 14.4 0 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
LDSEFQPLSSAFR + Deamidated (NQ)
4050 769.7117 2306.1133 2306.1253 -5.21 0 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
TVNGGVFMNDEYLSHVILPGK + Deamidated (NQ); Oxidation (M)
3021 759.8696 1517.7246 1517.7110 8.96 0 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
STLDWPGGASEVSGR
3153 803.4236 1604.8326 1604.8443 -7.28 1 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
LSDELKGDLSIIMR + Oxidation (M)
1941 503.7528 1005.4910 1005.5025 -11.4 1 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
REVGASMTR
2427 567.7832 1133.5518 1133.5452 5.88 0 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
LVTDNSVEEK + Deamidated (NQ)
2429 1135.5287 1134.5214 1134.5413 -17.5 0 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
INLEMCDIK
2742 637.3758 1272.7370 1272.7554 -14.4 1 1 2.1 +1Score > 16 indicates identity LPKLNVHLNPK + Deamidated (NQ)
2824 685.3215 1368.6284 1368.6265 1.44 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
SMVKVMLNDNGK + 2 Deamidated (NQ); 2 Oxidation (M)
3195 818.3994 1634.7842 1634.7933 -5.57 1 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
AGSNTMNKVSGLDIAR + 2 Deamidated (NQ)
4263 690.0931 2756.3433 2756.3641 -7.54 1 1 0.98 +1Score > 22 indicates identity
Score > 13 indicates homology
WLTFTMLILLITSHCCYWNKR + Deamidated (NQ)
3000 753.8588 1505.7030 1505.6919 7.39 0 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
VAVQQTQMQAGVDK + 4 Deamidated (NQ)
4042 768.3813 2302.1221 2302.1250 -1.26 1 1 0.98 +1Score > 22 indicates identity
Score > 13 indicates homology
AGLNMTKVDLLIGPNDEDELK + 2 Deamidated (NQ); Oxidation (M)
1182 687.4040 686.3967 686.3963 0.66 0 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
DLLAQK
2003 512.2512 1022.4878 1022.4743 13.3 0 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
YPGNPTMVK + Deamidated (NQ); Oxidation (M)
4486 1075.5402 3223.5988 3223.6508 -16.1 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
KPQVLKELENLLQMFEEGLPCIEHALK + 2 Deamidated (NQ); Oxidation (M)
4709 957.2027 6693.3680 6693.4451 -11.5 1 1 1.1 +1Score > 14 indicates identity DVLTQWWVTLLNFLNSDTSLQIDTALELSLSIELTSVCLECLSKIMTILIILPFHSSR + 4 Deamidated (NQ)
3159 539.5740 1615.7002 1615.7114 -6.95 1 1 4 +1Score > 19 indicates identity FKQEFSNAASGSGER + 2 Deamidated (NQ)
2898 720.8370 1439.6594 1439.6780 -12.9 1 1 0.95 +1Score > 21 indicates identity
Score > 13 indicates homology
STEPVSASDKYQK + Deamidated (NQ)
2614 590.2883 1178.5620 1178.5819 -16.9 1 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
KDGLDDQFLK + Deamidated (NQ)
4113 794.7232 2381.1478 2381.1937 -19.3 0 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
LKPPAGSQFIINDSIMSYIDR + Deamidated (NQ); Oxidation (M)
1625 456.2321 910.4496 910.4508 -1.30 0 1 4.1 +1Score > 19 indicates identity DLEVNGHK
1945 504.2640 1006.5134 1006.5137 -0.25 1 1 0.93 +1Score > 24 indicates identity
Score > 13 indicates homology
WRAWFNK
2970 495.9148 1484.7226 1484.7367 -9.52 1 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
LRFLMDTMSDLK + Oxidation (M)
1038 606.2745 605.2672 605.2744 -11.9 0 1 4 +1Score > 19 indicates identity WGMGR
2241 542.8226 1083.6306 1083.6400 -8.67 1 1 2.6 +1Score > 17 indicates identity EGVGKAVVGLR
4450 767.4041 3065.5873 3065.5339 17.4 1 1 3.7 +1Score > 19 indicates identity SSNNDQLIESSLFIDIANTSMILRDIR + Deamidated (NQ)
4235 898.4611 2692.3615 2692.3702 -3.26 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
KPMNEIENLEALASEIQYVMLKK + 2 Deamidated (NQ)
1282 748.3997 747.3924 747.3915 1.20 0 1 0.89 +1Score > 21 indicates identity
Score > 13 indicates homology
WSIQSK
2738 636.3037 1270.5928 1270.5789 11.0 0 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
NGQASAPRPNQK + 4 Deamidated (NQ)
2720 629.3223 1256.6300 1256.6071 18.3 0 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
NQMAHLVDLAK + 2 Deamidated (NQ); Oxidation (M)
2988 750.4126 1498.8106 1498.8395 -19.2 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
LDLINHEKYLLK + Deamidated (NQ)
2387 1126.5139 1125.5066 1125.5237 -15.2 0 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
CTINGVSYGR
2324 1106.5864 1105.5791 1105.5914 -11.1 1 1 0.94 +1Score > 22 indicates identity
Score > 13 indicates homology
TAALISKSCR
2719 419.8835 1256.6287 1256.6336 -3.89 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
KANQPIVHYGM
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