MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4601–4700 (out of 4700)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4618 1086.2567 4340.9977
4619 1094.8486 4375.3653
4620 1100.5024 4397.9805
4621 1100.7629 4399.0225
4622 1104.3088 4413.2061
4623 633.9404 4430.5319
4624 633.9406 4430.5333
4625 634.0820 4431.5231
4626 634.0831 4431.5308
4627 634.0832 4431.5315
4628 739.5961 4431.5329
4629 739.5963 4431.5341
4630 739.5989 4431.5497
4631 739.7609 4432.5217
4632 901.8743 4504.3351
4633 901.8799 4504.3631
4634 1127.3429 4505.3425
4635 752.0165 4506.0553
4636 752.0190 4506.0703
4637 752.0208 4506.0811
4638 752.0217 4506.0865
4639 752.1878 4507.0831
4640 752.1890 4507.0903
4642 752.1895 4507.0933
4643 752.3787 4508.2285
4645 1145.3274 4577.2805
4646 939.5150 4692.5386
4647 997.5228 4982.5776
4648 997.7159 4983.5431
4649 834.4529 5000.6737
4650 1009.5357 5042.6421
4652 1021.7556 5103.7416
4653 1063.5203 5312.5651
4654 1093.5289 5462.6081
4655 1100.5317 5497.6221
4657 1105.1560 5520.7436
4658 922.8248 5530.9051
4659 1107.3871 5531.8991
4660 922.9935 5531.9173
4661 925.1583 5544.9061
4662 1109.9915 5544.9211
4663 925.3274 5545.9207
4664 1110.1967 5545.9471
4665 1112.7948 5558.9376
4666 927.4971 5558.9389
4667 927.6624 5559.9307
4668 1112.9939 5559.9331
4670 796.8494 5570.8949
4671 796.8501 5570.8998
4672 697.3719 5570.9170
4673 796.8549 5570.9334
4674 929.4962 5570.9335
4675 697.3774 5570.9610
4676 796.9955 5571.9176
4677 930.3316 5575.9459
4680 1118.5363 5587.6451
4682 1118.5424 5587.6756
4683 933.8215 5596.8853
4684 800.7036 5597.8743
4685 934.6614 5601.9247
4686 936.3140 5611.8403
4687 936.8268 5614.9171
4688 937.1641 5616.9409
4689 937.4554 5618.6887
4690 1124.7483 5618.7051
4691 1005.8750 6029.2063
4692 1008.0463 6042.2341
4693 864.3229 6043.2094
4694 864.3287 6043.2500
4696 866.1837 6056.2350
4697 1010.3802 6056.2375
4698 1010.3806 6056.2399
4699 866.1846 6056.2413
4700 779.5427 6228.2834
4701 779.6679 6229.2850
4702 779.6705 6229.3058
4703 779.7968 6230.3162
4704 891.1921 6231.2938
4705 1050.0443 6294.2221
4706 908.1857 6350.2490
4707 908.7443 6354.1592
4708 918.4814 6422.3189
4710 964.4753 6744.2762
4711 844.0430 6744.2858
4712 964.6178 6745.2737
4713 750.4846 6745.2959
4714 964.6227 6745.3080
4715 844.1713 6745.3122
4716 964.6261 6745.3318
4717 964.7639 6746.2964
4718 844.4191 6747.2946
4719 844.4197 6747.2994
4720 986.5094 6898.5149
4721 863.3298 6898.5802
4722 986.5193 6898.5842
4723 863.4506 6899.5466
4724 863.4533 6899.5682
4725 986.6625 6899.5866
4726 863.5792 6900.5754
4728 910.4803 9094.7302
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