MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4501–4600 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4507 1116.4910 3346.4512
4508 1116.5072 3346.4998
4509 1116.8225 3347.4457
4510 1117.1879 3348.5419
4511 1117.5253 3349.5541
4512 1122.5452 3364.6138
4514 1123.5463 3367.6171
4515 1124.1503 3369.4291
4516 1124.4705 3370.3897
4517 1129.1362 3384.3868
4518 1129.4623 3385.3651
4519 1132.8743 3395.6011
4520 1135.5004 3403.4794
4521 1135.5416 3403.6030
4522 1135.5507 3403.6303
4523 1135.8378 3404.4916
4525 1136.5536 3406.6390
4526 1140.5375 3418.5907
4527 1140.8679 3419.5819
4528 1141.5546 3421.6420
4529 1141.5577 3421.6513
4530 1142.2089 3423.6049
4531 857.4343 3425.7081
4532 858.4329 3429.7025
4533 858.4371 3429.7193
4534 1144.2606 3429.7600
4535 1144.5880 3430.7422
4536 1144.5928 3430.7566
4537 1144.5999 3430.7779
4539 861.4523 3441.7801
4540 861.6827 3442.7017
4541 865.4332 3457.7037
4542 865.4395 3457.7289
4543 1165.5751 3493.7035
4544 875.4435 3497.7449
4545 1170.2297 3507.6673
4546 1175.5660 3523.6762
4547 1175.5701 3523.6885
4548 1176.5582 3526.6528
4549 1178.9049 3533.6929
4550 884.4321 3533.6993
4551 1179.5690 3535.6852
4552 887.4188 3545.6461
4554 888.4255 3549.6729
4558 1184.5691 3550.6855
4559 1184.5721 3550.6945
4560 1188.5408 3562.6006
4561 1189.5710 3565.6912
4562 892.4347 3565.7097
4563 1189.8960 3566.6662
4564 1197.2273 3588.6601
4565 906.7416 3622.9373
4566 911.9890 3643.9269
4567 914.4551 3653.7913
4568 732.9843 3659.8851
4569 916.2371 3660.9193
4570 736.4037 3676.9821
4571 920.7042 3678.7877
4572 920.7452 3678.9517
4573 934.0520 3732.1789
4574 934.2266 3732.8773
4575 938.7702 3751.0517
4576 973.2475 3888.9609
4577 973.5021 3889.9793
4578 990.9637 3959.8257
4580 810.4203 4047.0651
4581 810.4248 4047.0876
4582 1013.0226 4048.0613
4583 1013.0232 4048.0637
4584 1013.0235 4048.0649
4585 1013.0247 4048.0697
4586 1013.0248 4048.0701
4587 1013.0259 4048.0745
4588 1013.0264 4048.0765
4589 1013.0273 4048.0801
4590 1013.0280 4048.0829
4591 1013.0297 4048.0897
4592 1013.0298 4048.0901
4593 1016.5273 4062.0801
4594 1016.7698 4063.0501
4595 1016.7707 4063.0537
4596 1016.7752 4063.0717
4598 1016.7771 4063.0793
4600 1044.0598 4172.2101
4601 597.3466 4174.3753
4602 696.9007 4175.3605
4603 696.9020 4175.3683
4604 696.9023 4175.3701
4605 696.9026 4175.3719
4606 696.9035 4175.3773
4607 836.2777 4176.3521
4608 697.0675 4176.3613
4609 697.2448 4177.4251
4610 1045.8129 4179.2225
4611 1051.5398 4202.1301
4612 1053.5260 4210.0749
4613 1060.3479 4237.3625
4614 1066.5155 4262.0329
4616 866.6751 4328.3391
4617 1084.3215 4333.2569
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