MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4401–4500 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4398 988.4603 2962.3591
4399 741.8411 2963.3353
4400 989.4772 2965.4098
4401 989.8279 2966.4619
4402 742.8748 2967.4701
4404 990.4900 2968.4482
4405 990.5156 2968.5250
4406 990.5259 2968.5559
4407 991.8246 2972.4520
4408 744.3727 2973.4617
4409 993.4436 2977.3090
4411 994.8420 2981.5042
4412 995.1622 2982.4648
4413 995.1663 2982.4771
4414 995.4474 2983.3204
4415 995.4582 2983.3528
4416 995.4935 2983.4587
4417 995.4964 2983.4674
4418 746.8756 2983.4733
4419 995.8187 2984.4343
4420 748.6417 2990.5377
4421 998.4335 2992.2787
4422 998.4460 2992.3162
4423 750.1287 2996.4857
4424 999.8415 2996.5027
4425 1000.1356 2997.3850
4426 1000.4882 2998.4428
4427 1000.5044 2998.4914
4429 1005.1588 3012.4546
4430 1005.2133 3012.6181
4431 754.3727 3013.4617
4432 1005.8303 3014.4691
4433 1006.2312 3015.6718
4434 1006.2353 3015.6841
4435 1006.4650 3016.3732
4436 755.1732 3016.6637
4437 1006.5673 3016.6801
4438 1008.2134 3021.6184
4439 1009.1669 3024.4789
4440 1010.4587 3028.3543
4441 1010.5529 3028.6369
4442 1010.5593 3028.6561
4443 1012.1750 3033.5032
4444 1015.8350 3044.4832
4445 1016.2133 3045.6181
4446 1017.8781 3050.6125
4447 1018.8470 3053.5192
4448 1020.2217 3057.6433
4449 1020.4891 3058.4455
4451 1025.8204 3074.4394
4452 1026.1228 3075.3466
4453 1026.5028 3076.4866
4454 1028.5181 3082.5325
4455 1028.8499 3083.5279
4456 1029.5139 3085.5199
4457 1029.5640 3085.6702
4459 776.1312 3100.4957
4460 1034.5120 3100.5142
4461 776.4268 3101.6781
4462 1035.8330 3104.4772
4464 1039.8444 3116.5114
4465 1040.5077 3118.5013
4467 1048.7942 3143.3608
4468 1053.5101 3157.5085
4469 1053.8459 3158.5159
4470 792.4218 3165.6581
4471 792.4244 3165.6685
4472 792.4245 3165.6689
4473 792.6722 3166.6597
4474 1063.1294 3186.3664
4475 1063.9167 3188.7283
4476 1064.2290 3189.6652
4477 1065.1752 3192.5038
4478 1065.5103 3193.5091
4479 1067.4603 3199.3591
4480 1068.1298 3201.3676
4481 1069.8274 3206.4604
4483 803.4183 3209.6441
4484 1071.5532 3211.6378
4485 1075.1567 3222.4483
4487 808.3826 3229.5013
4488 1077.8469 3230.5189
4489 1080.1888 3237.5446
4490 1080.2170 3237.6292
4491 1084.5266 3250.5580
4492 1085.9044 3254.6914
4493 1086.2252 3255.6538
4494 1090.1932 3267.5578
4495 1090.5829 3268.7269
4496 1092.5221 3274.5445
4497 1096.5223 3286.5451
4498 1097.4839 3289.4299
4499 1097.4968 3289.4686
4500 1097.5192 3289.5358
4501 1097.8123 3290.4151
4502 1097.8475 3290.5207
4503 1098.2096 3291.6070
4504 1098.5419 3292.6039
4505 1110.5386 3328.5940
4506 1111.4878 3331.4416
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