MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4301–4400 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4290 931.4536 2791.3390
4291 932.7814 2795.3224
4292 932.7831 2795.3275
4293 933.1498 2796.4276
4294 701.3411 2801.3353
4296 935.1255 2802.3547
4297 935.1284 2802.3634
4298 935.1304 2802.3694
4299 935.1305 2802.3697
4300 935.1335 2802.3787
4301 935.4125 2803.2157
4302 935.4583 2803.3531
4303 935.4676 2803.3810
4304 701.8621 2803.4193
4305 702.8937 2807.5457
4306 702.8964 2807.5565
4308 938.4434 2812.3084
4309 938.4509 2812.3309
4310 938.4610 2812.3612
4311 938.4706 2812.3900
4312 705.3323 2817.3001
4313 940.4386 2818.2940
4314 940.4554 2818.3444
4315 942.1321 2823.3745
4316 942.1347 2823.3823
4317 942.4726 2824.3960
4318 943.1349 2826.3829
4319 943.5221 2827.5445
4320 943.7799 2828.3179
4321 943.7876 2828.3410
4322 945.4260 2833.2562
4323 945.7725 2834.2957
4324 945.8597 2834.5573
4325 946.1902 2835.5488
4326 946.4524 2836.3354
4327 947.1418 2838.4036
4328 947.4780 2839.4122
4329 949.1182 2844.3328
4331 950.7637 2849.2693
4332 951.1392 2850.3958
4333 951.4674 2851.3804
4334 951.7891 2852.3455
4337 953.1010 2856.2812
4338 953.4766 2857.4080
4339 953.4866 2857.4380
4340 716.3378 2861.3221
4341 955.4796 2863.4170
4342 956.0867 2865.2383
4344 957.4590 2869.3552
4345 718.3833 2869.5041
4346 718.3857 2869.5137
4347 718.6321 2870.4993
4348 959.8394 2876.4964
4350 962.1568 2883.4486
4351 962.4521 2884.3345
4352 962.7943 2885.3611
4353 962.8159 2885.4259
4354 962.8646 2885.5720
4355 963.1386 2886.3940
4356 963.2007 2886.5803
4357 964.4497 2890.3273
4358 965.5189 2893.5349
4359 967.4772 2899.4098
4360 968.4734 2902.3984
4361 969.7691 2906.2855
4362 971.7746 2912.3020
4363 972.0967 2913.2683
4364 972.1102 2913.3088
4365 972.4242 2914.2508
4366 972.4255 2914.2547
4367 972.4371 2914.2895
4368 972.4374 2914.2904
4369 972.8507 2915.5303
4370 973.4536 2917.3390
4371 974.1947 2919.5623
4372 975.1639 2922.4699
4373 975.4695 2923.3867
4374 975.4706 2923.3900
4375 975.8292 2924.4658
4376 977.7675 2930.2807
4377 977.7682 2930.2828
4378 980.4837 2938.4293
4379 980.8004 2939.3794
4380 980.8157 2939.4253
4381 981.1298 2940.3676
4382 981.1661 2940.4765
4383 982.4784 2944.4134
4385 982.4821 2944.4245
4386 982.8149 2945.4229
4387 983.4565 2947.3477
4388 984.4984 2950.4734
4389 985.4867 2953.4383
4390 986.1285 2955.3637
4391 986.4695 2956.3867
4392 986.4811 2956.4215
4393 986.4895 2956.4467
4394 986.4927 2956.4563
4395 740.3709 2957.4545
4396 987.4673 2959.3801
4397 988.4499 2962.3279
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