MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4201–4300 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4180 844.7719 2531.2939
4182 847.4053 2539.1941
4183 847.7327 2540.1763
4184 849.0865 2544.2377
4185 849.0878 2544.2416
4186 853.4176 2557.2310
4187 854.7139 2561.1199
4188 855.3799 2563.1179
4189 855.7066 2564.0980
4190 857.4324 2569.2754
4191 858.0622 2571.1648
4192 860.0757 2577.2053
4193 860.4085 2578.2037
4194 862.7222 2585.1448
4195 862.7626 2585.2660
4196 863.3933 2587.1581
4197 863.4023 2587.1851
4198 864.4946 2590.4620
4199 865.1445 2592.4117
4200 865.4075 2593.2007
4201 866.4051 2596.1935
4202 866.4186 2596.2340
4203 866.7346 2597.1820
4204 867.0907 2598.2503
4205 867.4121 2599.2145
4206 867.4247 2599.2523
4207 867.4254 2599.2544
4208 868.3912 2602.1518
4209 868.4038 2602.1896
4210 869.0886 2604.2440
4211 871.4089 2611.2049
4212 872.0688 2613.1846
4213 872.4039 2614.1899
4214 872.4200 2614.2382
4215 873.0973 2616.2701
4217 876.4364 2626.2874
4218 876.7327 2627.1763
4219 877.1375 2628.3907
4220 878.1125 2631.3157
4222 883.8059 2648.3959
4223 663.6130 2650.4229
4224 885.4553 2653.3441
4225 885.4648 2653.3726
4227 889.4159 2665.2259
4228 891.4266 2671.2580
4230 896.4493 2686.3261
4231 896.4680 2686.3822
4232 898.4481 2692.3225
4233 898.4494 2692.3264
4234 898.4583 2692.3531
4236 898.8456 2693.5150
4237 900.1097 2697.3073
4238 900.1198 2697.3376
4240 901.4380 2701.2922
4241 676.6329 2702.5025
4242 905.1202 2712.3388
4243 908.8456 2723.5150
4244 910.1353 2727.3841
4245 910.4652 2728.3738
4246 912.4315 2734.2727
4247 912.4633 2734.3681
4249 912.4676 2734.3810
4250 912.7610 2735.2612
4251 912.7921 2735.3545
4252 913.7877 2738.3413
4253 915.1449 2742.4129
4254 915.1472 2742.4198
4255 917.8427 2750.5063
4256 688.6339 2750.5065
4257 917.8432 2750.5078
4258 917.8433 2750.5081
4259 917.8442 2750.5108
4260 688.6353 2750.5121
4261 918.1707 2751.4903
4262 918.1752 2751.5038
4264 920.1080 2757.3022
4265 690.3364 2757.3165
4266 920.7648 2759.2726
4267 922.1347 2763.3823
4268 922.1367 2763.3883
4269 922.1371 2763.3895
4270 923.0657 2766.1753
4271 924.8392 2771.4958
4272 925.1658 2772.4756
4273 925.1768 2772.5086
4274 694.3761 2773.4753
4275 925.8398 2774.4976
4276 925.8417 2774.5033
4277 926.1761 2775.5065
4278 926.4370 2776.2892
4279 928.1196 2781.3370
4280 929.0776 2784.2110
4282 929.4683 2785.3831
4283 929.5079 2785.5019
4284 930.1255 2787.3547
4285 558.6989 2788.4581
4286 558.6993 2788.4601
4287 698.3715 2789.4569
4288 698.3729 2789.4625
4289 931.1840 2790.5302
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