MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4101–4200 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4062 1157.5681 2313.1216
4063 772.3607 2314.0603
4064 1159.5424 2317.0702
4065 774.0638 2319.1696
4067 775.3810 2323.1212
4068 1162.5879 2323.1612
4069 775.4030 2323.1872
4070 1163.0682 2324.1218
4072 1163.0703 2324.1260
4073 776.3727 2326.0963
4074 776.4073 2326.2001
4075 1165.5544 2329.0942
4076 777.3741 2329.1005
4077 1165.5576 2329.1006
4079 777.7123 2330.1151
4080 779.3726 2335.0960
4081 1168.5577 2335.1008
4082 779.6995 2336.0767
4083 1169.5485 2337.0824
4084 780.4711 2338.3915
4085 1170.5984 2339.1822
4086 781.3718 2341.0936
4087 781.6985 2342.0737
4088 783.0345 2346.0817
4089 587.5278 2346.0821
4090 783.3726 2347.0960
4092 784.4595 2350.3567
4093 784.6938 2351.0596
4094 1176.5485 2351.0824
4095 784.7023 2351.0851
4096 1176.5615 2351.1084
4097 785.0714 2352.1924
4098 785.7082 2354.1028
4099 789.0610 2364.1612
4100 789.3832 2365.1278
4101 1183.5767 2365.1388
4102 789.3945 2365.1617
4105 790.0271 2367.0595
4106 1184.5469 2367.0792
4107 790.0345 2367.0817
4108 790.7899 2369.3479
4109 593.7970 2371.1589
4110 791.3945 2371.1617
4111 791.3984 2371.1734
4112 1191.5791 2381.1436
4114 795.0318 2382.0736
4115 1192.0734 2382.1322
4116 795.0529 2382.1369
4117 795.6993 2384.0761
4118 796.6895 2387.0467
4121 599.3075 2393.2009
4123 800.0302 2397.0688
4124 800.7252 2399.1538
4125 803.7087 2408.1043
4126 805.1280 2412.3622
4127 805.1310 2412.3712
4128 805.3582 2413.0528
4130 807.3846 2419.1320
4132 809.3698 2425.0876
4133 809.3991 2425.1755
4135 811.6980 2432.0722
4137 814.0692 2439.1858
4138 814.7357 2441.1853
4139 814.7811 2441.3215
4140 817.1359 2448.3859
4141 819.4177 2455.2313
4142 819.4195 2455.2367
4143 820.0605 2457.1597
4144 820.1393 2457.3961
4145 821.1118 2460.3136
4146 822.1097 2463.3073
4148 823.1249 2466.3529
4149 823.1254 2466.3544
4150 823.3972 2467.1698
4151 824.7487 2471.2243
4152 824.7502 2471.2288
4153 826.3896 2476.1470
4155 827.1293 2478.3661
4156 827.1379 2478.3919
4157 828.4072 2482.1998
4158 829.0574 2484.1504
4160 830.0799 2487.2179
4161 830.4229 2488.2469
4162 830.4516 2488.3330
4163 834.3876 2500.1410
4164 834.7927 2501.3563
4165 835.4332 2503.2778
4166 835.7065 2504.0977
4167 837.1121 2508.3145
4168 837.3959 2509.1659
4169 837.3993 2509.1761
4170 837.4465 2509.3177
4171 838.4253 2512.2541
4173 630.0898 2516.3301
4174 839.7871 2516.3395
4175 842.4023 2524.1851
4176 843.0586 2526.1540
4177 843.3675 2527.0807
4178 843.4204 2527.2394
4179 843.7570 2528.2492
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