MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4001–4100 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3948 1099.0046 2195.9946
3949 733.3350 2196.9832
3950 1099.5052 2196.9958
3951 1099.5276 2197.0406
3952 1100.5171 2199.0196
3953 734.7386 2201.1940
3954 1102.0482 2202.0818
3955 735.3716 2203.0930
3956 1102.9902 2203.9658
3957 1103.5419 2205.0692
3958 1105.0361 2208.0576
3959 737.0269 2208.0589
3960 1105.5072 2208.9998
3961 737.3872 2209.1398
3962 1106.5021 2210.9896
3963 1107.4906 2212.9666
3964 738.6677 2212.9813
3965 1107.5327 2213.0508
3966 739.6545 2215.9417
3967 1108.9797 2215.9448
3969 1109.4869 2216.9592
3970 740.3580 2218.0522
3971 1110.0336 2218.0526
3972 1110.0415 2218.0684
3973 740.9841 2219.9305
3974 742.3402 2223.9988
3975 742.3484 2224.0234
3976 1113.5157 2225.0168
3977 1113.5298 2225.0450
3978 743.3550 2227.0432
3979 1114.5325 2227.0504
3980 1115.5321 2229.0496
3981 1116.4817 2230.9488
3982 1117.5269 2233.0392
3983 1120.5099 2239.0052
3984 1122.5168 2243.0190
3985 748.9946 2243.9620
3987 1124.1190 2246.2234
3988 750.0863 2247.2371
3989 750.3723 2248.0951
3991 751.0366 2250.0880
3992 751.0380 2250.0922
3993 1126.0616 2250.1086
3994 751.0508 2250.1306
3995 1126.5435 2251.0724
3996 751.3781 2251.1125
3997 1126.5643 2251.1140
3998 751.3799 2251.1179
3999 751.3805 2251.1197
4000 751.4459 2251.3159
4001 751.7802 2252.3188
4002 1128.0225 2254.0304
4003 1132.5226 2263.0306
4004 755.3515 2263.0327
4005 756.3658 2266.0756
4006 756.3777 2266.1113
4007 1134.5604 2267.1062
4008 757.3441 2269.0105
4009 1135.5140 2269.0134
4010 758.3452 2272.0138
4011 1138.5446 2275.0746
4012 760.7161 2279.1265
4013 1145.5668 2289.1190
4014 1146.5616 2291.1086
4015 764.7148 2291.1226
4016 1146.5754 2291.1362
4017 1146.5822 2291.1498
4018 764.7245 2291.1517
4019 765.4166 2293.2280
4020 765.4174 2293.2304
4021 765.4174 2293.2304
4022 765.4185 2293.2337
4023 765.4208 2293.2406
4024 765.4213 2293.2421
4025 765.4222 2293.2448
4026 765.4223 2293.2451
4027 765.4228 2293.2466
4028 765.4232 2293.2478
4029 765.4318 2293.2736
4030 765.7531 2294.2375
4031 766.3641 2296.0705
4033 766.7093 2297.1061
4034 766.7518 2297.2336
4035 1150.0239 2298.0332
4036 767.0186 2298.0340
4037 1150.0612 2298.1078
4038 767.4033 2299.1881
4040 1151.5675 2301.1204
4047 1152.5833 2303.1520
4048 769.0563 2304.1471
4049 1153.5679 2305.1212
4051 1154.5709 2307.1272
4052 770.0509 2307.1309
4053 770.3816 2308.1230
4054 1155.0708 2308.1270
4055 1155.0719 2308.1292
4056 770.3852 2308.1338
4057 770.3862 2308.1368
4059 1157.5654 2313.1162
4061 772.0477 2313.1213
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