MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3901–4000 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3835 1057.4976 2112.9806
3836 1057.5171 2113.0196
3837 1057.5175 2113.0204
3838 705.3566 2113.0480
3839 1058.0236 2114.0326
3840 705.6882 2114.0428
3841 705.6917 2114.0533
3842 705.7249 2114.1529
3843 1058.5037 2114.9928
3844 1058.5070 2114.9994
3846 1058.5872 2115.1598
3847 706.9987 2117.9743
3848 1060.1935 2118.3724
3849 1060.4950 2118.9754
3850 1060.4958 2118.9770
3851 707.3344 2118.9814
3852 1061.4857 2120.9568
3853 1061.4880 2120.9614
3855 1061.5134 2121.0122
3856 1062.5320 2123.0494
3857 708.9984 2123.9734
3858 1063.0374 2124.0602
3859 1063.4829 2124.9512
3860 1064.4838 2126.9530
3861 1065.4969 2128.9792
3862 1065.5023 2128.9900
3863 1065.5118 2129.0090
3864 1066.0172 2130.0198
3865 1066.4701 2130.9256
3866 1066.5065 2130.9984
3867 1066.5190 2131.0234
3868 711.6455 2131.9147
3869 1067.5045 2132.9944
3870 712.0059 2132.9959
3871 712.0212 2133.0418
3874 1068.4922 2134.9698
3875 712.6655 2134.9747
3876 712.6666 2134.9780
3877 1068.5015 2134.9884
3878 1068.5105 2135.0064
3880 712.6985 2135.0737
3881 1068.9869 2135.9592
3882 713.0103 2136.0091
3883 1069.0449 2136.0752
3884 713.0329 2136.0769
3885 713.3284 2136.9634
3886 1069.4899 2136.9652
3887 1069.5066 2136.9986
3888 1069.5146 2137.0146
3889 713.3515 2137.0327
3890 1071.4886 2140.9626
3891 1072.5116 2143.0086
3892 716.6384 2146.8934
3893 1075.4867 2148.9588
3894 1075.5015 2148.9884
3895 717.3373 2148.9901
3898 1076.4866 2150.9586
3899 1076.5120 2151.0094
3900 1076.5225 2151.0304
3901 718.0322 2151.0748
3902 1076.5468 2151.0790
3903 1077.4835 2152.9524
3904 718.6597 2152.9573
3905 719.9823 2156.9251
3906 1079.4824 2156.9502
3907 720.3193 2157.9361
3908 1080.5078 2159.0010
3909 1081.0254 2160.0362
3910 1081.4944 2160.9742
3912 1082.5021 2162.9896
3914 722.3632 2164.0678
3916 723.3315 2166.9727
3917 723.6544 2167.9414
3918 1085.4825 2168.9504
3919 1085.5527 2169.0908
3920 1085.5645 2169.1144
3921 1086.0347 2170.0548
3922 724.3774 2170.1104
3923 1086.9625 2171.9104
3925 725.3141 2172.9205
3926 725.6851 2174.0335
3927 1088.4915 2174.9684
3928 1088.5300 2175.0454
3929 1089.0663 2176.1180
3930 726.3843 2176.1311
3931 726.3973 2176.1701
3932 1090.5276 2179.0406
3933 1091.5547 2181.0948
3934 1091.9967 2181.9788
3935 1093.5466 2185.0786
3936 1094.5054 2186.9962
3938 1096.5706 2191.1266
3939 731.6688 2191.9846
3940 549.0037 2191.9857
3941 1097.0625 2192.1104
3942 1098.0002 2193.9858
3943 1098.0100 2194.0054
3944 732.3432 2194.0078
3945 732.3433 2194.0081
3946 1098.5082 2195.0018
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