MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 301–400 (out of 4700)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2274 1093.5078 1092.5005 1092.4948 5.23 0 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
QPPETNHNR + Deamidated (NQ)
2791 664.8306 1327.6466 1327.6408 4.41 0 4 0.58 +1Score > 22 indicates identity
Score > 14 indicates homology
NSATILNYFER + Deamidated (NQ)
960 529.2996 528.2923 528.2908 2.97 0 4 1.7 +1Score > 18 indicates identity VADPK
1032 602.3430 601.3357 601.3435 -12.9 0 4 0.75 +1Score > 23 indicates identity
Score > 15 indicates homology
ILNDK
1891 992.5466 991.5393 991.5233 16.2 1 4 1 +1Score > 22 indicates identity
Score > 16 indicates homology
MALSRSVGR + Oxidation (M)
2106 524.7817 1047.5488 1047.5461 2.58 1 4 1 +1Score > 22 indicates identity
Score > 16 indicates homology
ARGVEVDFR
1472 846.4427 845.4354 845.4429 -8.85 1 4 0.68 +1Score > 24 indicates identity
Score > 15 indicates homology
KDPIMAR + Oxidation (M)
3475 624.6149 1870.8229 1870.8405 -9.41 1 4 1.9 +1Score > 19 indicates identity NENKVNNATNATHNNSK + 2 Deamidated (NQ)
1568 889.3995 888.3922 888.3937 -1.67 0 4 0.92 +1Score > 16 indicates identity NQDEVER
907 487.2524 486.2451 486.2438 2.71 0 4 2.1 +1Score > 19 indicates identity APDGK
1524 870.4105 869.4032 869.4130 -11.3 0 4 1.3 +1Score > 17 indicates identity SLNASYSK + Deamidated (NQ)
1912 997.5243 996.5170 996.4988 18.3 1 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
QAAPRNPNK + 2 Deamidated (NQ)
3526 962.4270 1922.8394 1922.8204 9.93 0 4 1.4 +1Score > 18 indicates identity VADENWETCQQETLAK + 2 Deamidated (NQ)
938 502.3053 501.2980 501.2911 13.8 0 4 1 +1Score > 23 indicates identity
Score > 16 indicates homology
QGGIK
1489 427.7112 853.4078 853.4004 8.76 0 4 1.7 +1Score > 18 indicates identity FNQAVMK + Deamidated (NQ); Oxidation (M)
2737 636.2985 1270.5824 1270.5830 -0.42 1 4 0.59 +1Score > 20 indicates identity
Score > 14 indicates homology
KFQDSGFQGEK + Deamidated (NQ)
1474 848.3811 847.3738 847.3671 7.89 0 4 2 +1Score > 19 indicates identity LGNANSDR + 2 Deamidated (NQ)
1295 757.4202 756.4129 756.4017 14.8 0 4 2.2 +1Score > 19 indicates identity ENGIPVK + Deamidated (NQ)
1713 938.4231 937.4158 937.4215 -6.06 0 4 2.2 +1Score > 19 indicates identity WAVNTLMS + Deamidated (NQ); Oxidation (M)
2437 1137.5310 1136.5237 1136.5132 9.29 1 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
AAERDNMLTT + Oxidation (M)
2733 635.8063 1269.5980 1269.5911 5.50 1 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
STAKIDFNNMK + 2 Deamidated (NQ)
1476 848.4073 847.4000 847.4148 -17.4 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
NSNSTIGR
3139 400.9397 1599.7297 1599.7352 -3.41 0 3 0.76 +1Score > 20 indicates identity
Score > 15 indicates homology
VDNYTDAIVCFQR
1123 657.3577 656.3504 656.3493 1.68 0 3 1.1 +1Score > 16 indicates identity LGENPK
1076 628.3434 627.3361 627.3452 -14.5 1 3 0.64 +1Score > 20 indicates identity
Score > 14 indicates homology
RAEPR
1561 442.7288 883.4430 883.4440 -1.03 0 3 2.2 +1Score > 19 indicates identity LEQFGYK
2480 1147.5488 1146.5415 1146.5377 3.32 1 3 0.86 +1Score > 20 indicates identity
Score > 15 indicates homology
SKNSNVNNNR + Deamidated (NQ)
1504 431.2150 860.4154 860.4140 1.65 1 3 0.61 +1Score > 22 indicates identity
Score > 14 indicates homology
KEWNQR + Deamidated (NQ)
1322 386.2258 770.4370 770.4286 10.9 1 3 1.7 +1Score > 18 indicates identity NKQGVPK + Deamidated (NQ)
3495 947.4439 1892.8732 1892.8682 2.68 1 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
AMKEMQPQPGLVDIMR + 2 Deamidated (NQ); 3 Oxidation (M)
3584 656.9969 1967.9689 1967.9762 -3.70 1 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
GVDLEKLLEMSTEDFVK + Oxidation (M)
2866 703.8500 1405.6854 1405.6983 -9.16 1 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
IANNGTRLASMNK + Deamidated (NQ); Oxidation (M)
2447 380.5409 1138.6009 1138.5903 9.24 1 3 2.2 +1Score > 19 indicates identity MITSSKSLEK + Oxidation (M)
2899 720.8639 1439.7132 1439.6892 16.7 0 3 0.51 +1Score > 22 indicates identity
Score > 13 indicates homology
NNELHILDEQSK + Deamidated (NQ)
2934 728.3945 1454.7744 1454.7868 -8.48 0 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
VPENEAILLDTLK + Deamidated (NQ)
2215 1078.5040 1077.4967 1077.5104 -12.7 1 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
DFSFRHNR
2439 1137.5386 1136.5313 1136.5132 16.0 1 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
AAERDNMLTT + Oxidation (M)
3510 636.2982 1905.8728 1905.8738 -0.52 1 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
MSENNGNPLDLSSLRNK + 2 Deamidated (NQ); Oxidation (M)
2695 611.8293 1221.6440 1221.6209 18.9 1 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
MSKVMKPSNGK + Oxidation (M)
2975 745.8600 1489.7054 1489.6783 18.2 0 3 0.59 +1Score > 22 indicates identity
Score > 13 indicates homology
AVEALNDSELNGEK + 2 Deamidated (NQ)
1471 846.4072 845.3999 845.3991 0.95 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
NNSQQQK
2846 695.3430 1388.6714 1388.6646 4.96 1 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
KNIPEVDEWMK + Deamidated (NQ)
4248 912.4641 2734.3705 2734.3695 0.37 1 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
LSVSVEQVSIAKPMSNDGVSSAVERK + 2 Deamidated (NQ); Oxidation (M)
1906 498.7434 995.4722 995.4811 -8.90 0 3 0.8 +1Score > 21 indicates identity
Score > 15 indicates homology
ELSYDELK
1498 429.2083 856.4020 856.3967 6.25 0 3 1.6 +1Score > 18 indicates identity FVSDDFK
1097 644.3317 643.3244 643.3289 -6.99 0 3 1 +1Score > 16 indicates identity TQAPAR + Deamidated (NQ)
1239 723.3326 722.3253 722.3269 -2.15 0 3 2.2 +1Score > 19 indicates identity MSDNLK + Oxidation (M)
1091 641.3633 640.3560 640.3544 2.51 0 3 0.99 +1Score > 16 indicates identity NPSVPK
3016 757.9149 1513.8152 1513.8075 5.13 1 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
KPTMIKLQFHNR + 2 Deamidated (NQ)
4384 982.4807 2944.4203 2944.4600 -13.5 1 3 0.77 +1Score > 22 indicates identity
Score > 15 indicates homology
SLGLDVPRPMQVDFSNTLQKNADALGR + 3 Deamidated (NQ)
1435 833.4102 832.4029 832.4151 -14.6 1 3 1 +1Score > 23 indicates identity
Score > 16 indicates homology
NRTGTQR + Deamidated (NQ)
2540 580.7850 1159.5554 1159.5770 -18.6 0 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
MVPTMFPPPK + Oxidation (M)
4482 1070.8824 3209.6254 3209.6642 -12.1 1 3 2.4 +1Score > 19 indicates identity EASEILAGLQGILPMDISVHQVDGGLKVYR + 2 Deamidated (NQ)
1394 406.2257 810.4368 810.4236 16.4 0 3 2.4 +1Score > 19 indicates identity LQNDPPK
2216 1078.5118 1077.5045 1077.5104 -5.47 1 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
DFSFRHNR
2829 688.3416 1374.6686 1374.6779 -6.74 0 3 1 +1Score > 23 indicates identity
Score > 16 indicates homology
VQAVNSGYIASHK + 2 Deamidated (NQ)
2680 607.8278 1213.6410 1213.6415 -0.36 1 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
SNQADSLPVRK
1865 493.7317 985.4488 985.4651 -16.5 0 3 1.5 +1Score > 17 indicates identity EDALMVHR + Oxidation (M)
3266 564.3004 1689.8794 1689.8542 14.9 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
KIICPSVESISNGMR
2777 658.3102 1314.6058 1314.6164 -8.02 0 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
AQPSSGTTNAQPR + Deamidated (NQ)
2011 1024.5107 1023.5034 1023.5138 -10.1 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
GGAGNIFFNK
1252 730.3386 729.3313 729.3406 -12.7 0 3 1 +1Score > 15 indicates identity QSDQPR
2652 596.8052 1191.5958 1191.5805 12.9 1 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
MKELELQER + Deamidated (NQ); Oxidation (M)
4154 620.0781 2476.2833 2476.2818 0.60 1 3 0.57 +1Score > 21 indicates identity
Score > 13 indicates homology
VCIEEMITSGIILFHARSTGVK + Oxidation (M)
3505 952.9805 1903.9464 1903.9713 -13.1 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
DLGLPQSAFSIQDLLMR + Deamidated (NQ)
1104 323.1718 644.3290 644.3242 7.57 0 3 2.3 +1Score > 19 indicates identity NGEGIR
3482 940.4330 1878.8514 1878.8451 3.37 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
VRMQGEEQENIMIER + 2 Deamidated (NQ); Oxidation (M)
1467 845.4021 844.3948 844.4039 -10.7 0 3 0.57 +1Score > 20 indicates identity
Score > 13 indicates homology
EINEQGR
3129 797.3816 1592.7486 1592.7352 8.46 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
SGMIENGLQLSDNAK + Deamidated (NQ); Oxidation (M)
2894 717.3577 1432.7008 1432.7020 -0.82 1 3 0.93 +1Score > 22 indicates identity
Score > 15 indicates homology
MSFNAFASSLSKK + Oxidation (M)
1817 486.7362 971.4578 971.4672 -9.65 0 3 1 +1Score > 20 indicates identity
Score > 15 indicates homology
TGDGSAHISK
3469 932.9374 1863.8602 1863.8958 -19.0 0 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
ENALAIGELMMGTEEIK + Oxidation (M)
1142 669.3336 668.3263 668.3394 -19.6 0 3 2.3 +1Score > 19 indicates identity NWPPR
3468 932.4607 1862.9068 1862.9230 -8.67 1 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
VESPGMTGQIKSLNMAGK + Oxidation (M)
2763 434.2117 1299.6133 1299.6315 -14.0 0 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
SAYLAMCLSIR + Oxidation (M)
1021 589.2886 588.2813 588.2867 -9.16 0 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
GLNER + Deamidated (NQ)
2642 594.8128 1187.6110 1187.6299 -15.8 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
WQNTISNVVK
3056 769.8671 1537.7196 1537.7233 -2.37 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
RPDNQNPEGENLR
3873 712.3547 2134.0423 2134.0695 -12.7 1 3 1 +1Score > 23 indicates identity
Score > 15 indicates homology
LGQGTFGEVYKGIHLETQR + 2 Deamidated (NQ)
3068 774.4073 1546.8000 1546.7739 16.9 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
DAAPAKPPNQGLNPR + 2 Deamidated (NQ)
1861 984.5373 983.5300 983.5222 7.95 1 3 2.2 +1Score > 19 indicates identity ADHMAKLAK
4556 888.4355 3549.7129 3549.7144 -0.43 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
GVQSPPKPPPSNEISGTIENDVESIKQAADNMAK + 2 Deamidated (NQ)
2444 570.2690 1138.5234 1138.5142 8.12 0 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
SYSVNSEQPK + Deamidated (NQ)
2769 655.3257 1308.6368 1308.6323 3.47 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
ANLHGYGNPNPR
2107 524.7825 1047.5504 1047.5461 4.11 1 3 1 +1Score > 23 indicates identity
Score > 15 indicates homology
ARGVEVDFR
2842 462.5399 1384.5979 1384.5711 19.4 1 3 2 +1Score > 18 indicates identity MSNRDNESMLR + Deamidated (NQ); 2 Oxidation (M)
3489 630.6350 1888.8832 1888.8928 -5.11 1 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
GWNVRFNGAQLNANGNR + 2 Deamidated (NQ)
3491 946.4550 1890.8954 1890.8582 19.7 0 3 0.65 +1Score > 22 indicates identity
Score > 13 indicates homology
TVNELENTQDLLNQEK + 4 Deamidated (NQ)
3913 722.3436 2164.0090 2163.9920 7.84 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
GQLDLINDGEGSLSNFADNGK + Deamidated (NQ)
4103 790.0152 2367.0238 2366.9777 19.5 1 3 2.4 +1Score > 19 indicates identity MSETRMAQNMDTTDEQYLR + 3 Oxidation (M)
3032 762.3604 1522.7062 1522.7150 -5.78 1 3 0.74 +1Score > 21 indicates identity
Score > 14 indicates homology
ALREENEYEELK + Deamidated (NQ)
3265 564.2998 1689.8776 1689.8542 13.8 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
KIICPSVESISNGMR
3363 880.9325 1759.8504 1759.8264 13.6 0 2 1 +1Score > 22 indicates identity
Score > 15 indicates homology
SHSEEVIVPEFNSSAK + Deamidated (NQ)
2663 601.2907 1200.5668 1200.5887 -18.2 1 2 1 +1Score > 20 indicates identity
Score > 15 indicates homology
GRGGNTWINPK + 2 Deamidated (NQ)
1528 873.4205 872.4132 872.4174 -4.81 1 2 1.6 +1Score > 17 indicates identity KGHQMGAK + Deamidated (NQ); Oxidation (M)
1790 961.4717 960.4644 960.4763 -12.4 1 2 1 +1Score > 23 indicates identity
Score > 15 indicates homology
EANEKELK + Deamidated (NQ)
4131 809.0316 2424.0730 2424.0858 -5.29 1 2 1 +1Score > 20 indicates identity
Score > 15 indicates homology
MMTPPLNIAPNSNNSKSSIMNK + 4 Deamidated (NQ); 2 Oxidation (M)
4136 813.7839 2438.3299 2438.3382 -3.42 0 2 1 +1Score > 15 indicates identity GSGTLVVILAILMLGVAYYLLNE + Deamidated (NQ); Oxidation (M)
4599 1030.7863 4119.1161 4119.0748 10.0 1 2 2.7 +1Score > 19 indicates identity EKLDMYEQAISIELSSLIELIEQEEPSPEVWLTIK + Deamidated (NQ); Oxidation (M)
3800 696.3416 2086.0030 2085.9895 6.47 0 2 1 +1Score > 22 indicates identity
Score > 15 indicates homology
ETVFFANGNILYPEDISR + 2 Deamidated (NQ)
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