MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3801–3900 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3729 1029.4884 2056.9622
3732 1029.5065 2056.9984
3733 1030.4973 2058.9800
3734 1030.5244 2059.0342
3735 1030.5262 2059.0378
3736 687.3537 2059.0393
3737 687.3550 2059.0432
3738 1030.5306 2059.0466
3739 687.3615 2059.0627
3740 1030.5388 2059.0630
3742 688.0156 2061.0250
3743 688.3082 2061.9028
3744 688.3135 2061.9187
3745 688.3207 2061.9403
3746 1031.9811 2061.9476
3747 688.3758 2062.1056
3748 1032.4658 2062.9170
3749 688.6474 2062.9204
3750 688.9825 2063.9257
3751 1032.9736 2063.9326
3752 688.9874 2063.9404
3753 1032.9800 2063.9454
3754 1033.5090 2065.0034
3755 1033.5269 2065.0392
3756 1033.5320 2065.0494
3757 1034.5182 2067.0218
3758 1037.0016 2071.9886
3759 691.6708 2071.9906
3760 1037.4932 2072.9718
3762 1037.5482 2073.0818
3763 1037.5511 2073.0876
3764 1037.5541 2073.0936
3765 1038.0065 2073.9984
3766 692.3422 2074.0048
3767 1038.0433 2074.0720
3768 692.3657 2074.0753
3769 1038.0474 2074.0802
3771 692.3688 2074.0846
3772 692.3701 2074.0885
3773 692.3702 2074.0888
3774 1038.0537 2074.0928
3775 1038.0542 2074.0938
3776 692.3729 2074.0969
3777 1038.0563 2074.0980
3778 692.3756 2074.1050
3779 692.3870 2074.1392
3780 692.3876 2074.1410
3781 1038.4784 2074.9422
3782 1038.4908 2074.9670
3783 692.7198 2075.1376
3784 1038.9901 2075.9656
3785 1039.4823 2076.9500
3786 693.3273 2076.9601
3787 693.3381 2076.9925
3788 693.6334 2077.8784
3789 693.6406 2077.9000
3790 693.9801 2078.9185
3791 1040.4769 2078.9392
3792 694.3201 2079.9385
3793 1040.9768 2079.9390
3794 694.6757 2081.0053
3795 1042.0038 2081.9930
3796 1042.5100 2083.0054
3797 695.6726 2083.9960
3798 1043.4999 2084.9852
3799 1043.5040 2084.9934
3801 1045.4922 2088.9698
3802 1045.4977 2088.9808
3803 1045.9932 2089.9718
3804 697.6646 2089.9720
3805 1046.9863 2091.9580
3806 698.3270 2091.9592
3807 1047.4895 2092.9644
3808 698.9633 2093.8681
3809 699.3087 2094.9043
3810 1048.9742 2095.9338
3811 699.6528 2095.9366
3812 699.6699 2095.9879
3813 525.2551 2096.9913
3814 1050.0021 2097.9896
3815 700.3372 2097.9898
3816 1050.0343 2098.0540
3817 1050.4974 2098.9802
3818 1050.5050 2098.9954
3819 1050.5164 2099.0182
3820 1051.0159 2100.0172
3821 1051.5002 2100.9858
3822 701.3392 2100.9958
3823 1051.5063 2100.9980
3824 1052.4993 2102.9840
3825 702.3340 2103.9802
3826 1053.4873 2104.9600
3827 1053.4915 2104.9684
3828 702.9946 2105.9620
3829 1053.9899 2105.9652
3830 1055.4811 2108.9476
3831 703.9911 2108.9515
3832 1055.4844 2108.9542
3833 1056.9708 2111.9270
3834 705.0165 2112.0277
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