MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3701–3800 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3620 995.9792 1989.9438
3621 996.4575 1990.9004
3623 996.4650 1990.9154
3624 996.9633 1991.9120
3625 665.3292 1992.9658
3626 997.5007 1992.9868
3627 997.9602 1993.9058
3628 665.6489 1993.9249
3629 998.4460 1994.8774
3630 998.4523 1994.8900
3631 665.9800 1994.9182
3633 998.4794 1994.9442
3634 998.5064 1994.9982
3635 999.4577 1996.9008
3636 667.3185 1998.9337
3637 1000.4770 1998.9394
3638 667.3373 1998.9901
3639 1000.5029 1998.9912
3640 1000.9777 1999.9408
3641 1001.4717 2000.9288
3642 1002.4901 2002.9656
3643 1002.5429 2003.0712
3645 1002.5482 2003.0818
3646 669.2984 2004.8734
3647 669.3033 2004.8881
3648 1003.4514 2004.8882
3649 1003.4583 2004.9020
3650 669.3116 2004.9130
3651 1003.4672 2004.9198
3652 669.3214 2004.9424
3653 669.3416 2005.0030
3654 669.6408 2005.9006
3655 1003.9611 2005.9076
3656 1004.4723 2006.9300
3657 1004.4818 2006.9490
3658 670.0020 2006.9842
3659 1004.9600 2007.9054
3660 670.3094 2007.9064
3661 670.3102 2007.9088
3662 670.3273 2007.9601
3663 670.3369 2007.9889
3664 1005.0114 2008.0082
3666 1005.4996 2008.9846
3667 670.6693 2008.9861
3668 1005.5043 2008.9940
3669 1005.5161 2009.0176
3670 1005.5188 2009.0230
3671 670.7026 2009.0860
3672 1005.7110 2009.4074
3673 1006.5002 2010.9858
3674 1008.4919 2014.9692
3675 1009.4581 2016.9016
3676 1009.9665 2017.9184
3677 1010.4864 2018.9582
3678 1011.4477 2020.8808
3680 1011.4701 2020.9256
3681 1011.4705 2020.9264
3682 674.6497 2020.9273
3683 674.9629 2021.8669
3684 1011.9502 2021.8858
3685 674.9714 2021.8924
3687 675.0734 2022.1984
3688 675.0735 2022.1987
3689 1012.9705 2023.9264
3690 1013.2811 2024.5476
3691 1013.4926 2024.9706
3692 1013.4943 2024.9740
3693 675.9994 2024.9764
3694 676.0057 2024.9953
3695 1014.0060 2025.9974
3696 1014.5021 2026.9896
3697 1016.4887 2030.9628
3698 1016.5263 2031.0380
3699 678.3214 2031.9424
3700 679.3207 2034.9403
3701 1018.4786 2034.9426
3702 679.9596 2036.8570
3703 680.3024 2037.8854
3704 1020.4839 2038.9532
3705 1020.5176 2039.0206
3706 680.9958 2039.9656
3707 1021.0171 2040.0196
3708 1021.4506 2040.8866
3709 1021.4899 2040.9652
3710 1021.4906 2040.9666
3711 1022.4838 2042.9530
3714 682.3629 2044.0669
3715 683.0203 2046.0391
3716 683.3167 2046.9283
3717 1024.4730 2046.9314
3718 1024.4805 2046.9464
3719 1024.4812 2046.9478
3720 683.6518 2047.9336
3721 1024.9807 2047.9468
3722 1025.4778 2048.9410
3723 1026.9730 2051.9314
3724 684.9851 2051.9335
3725 1027.4822 2052.9498
3726 1028.5076 2055.0006
3727 1028.5114 2055.0082
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