MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3501–3600 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3397 600.6050 1798.7932
3398 900.4356 1798.8566
3399 900.8793 1799.7440
3400 601.2481 1800.7225
3401 902.4348 1802.8550
3402 602.5620 1804.6642
3403 903.3919 1804.7692
3404 903.3919 1804.7692
3405 903.4354 1804.8562
3406 602.9334 1805.7784
3407 904.4219 1806.8292
3409 904.9102 1807.8058
3410 605.2985 1812.8737
3412 907.9439 1813.8732
3413 908.8756 1815.7366
3414 908.9545 1815.8944
3415 909.3679 1816.7212
3416 910.4455 1818.8764
3417 910.9363 1819.8580
3418 607.9299 1820.7679
3420 911.8914 1821.7682
3421 911.9205 1821.8264
3422 912.0136 1822.0126
3423 912.9095 1823.8044
3424 912.9741 1823.9336
3425 914.4376 1826.8606
3426 915.4408 1828.8670
3427 917.4367 1832.8588
3428 917.4399 1832.8652
3429 611.9625 1832.8657
3430 917.4404 1832.8662
3431 918.4264 1834.8382
3432 918.4341 1834.8536
3433 918.9261 1835.8376
3435 919.4470 1836.8794
3436 919.8881 1837.7616
3437 920.3956 1838.7766
3438 920.4525 1838.8904
3439 920.9615 1839.9084
3440 614.3101 1839.9085
3441 921.4570 1840.8994
3442 921.9371 1841.8596
3443 615.2476 1842.7210
3444 922.8830 1843.7514
3445 616.2498 1845.7276
3446 923.9453 1845.8760
3447 924.4558 1846.8970
3448 924.4583 1846.9020
3449 925.4338 1848.8530
3450 617.2923 1848.8551
3451 925.4373 1848.8600
3452 925.9343 1849.8540
3453 618.2831 1851.8275
3454 926.9248 1851.8350
3455 926.9314 1851.8482
3456 927.4454 1852.8762
3457 927.4559 1852.8972
3458 927.4597 1852.9048
3459 927.9490 1853.8834
3460 928.9466 1855.8786
3461 929.4554 1856.8962
3462 929.4561 1856.8976
3463 620.5789 1858.7149
3464 930.4455 1858.8764
3465 930.9576 1859.9006
3466 931.4774 1860.9402
3467 931.9400 1861.8654
3470 933.4274 1864.8402
3471 623.0381 1866.0925
3472 934.5531 1867.0916
3473 623.3723 1867.0951
3474 935.4486 1868.8826
3477 624.9847 1871.9323
3478 626.3044 1875.8914
3479 938.9540 1875.8934
3480 939.4446 1876.8746
3481 940.4235 1878.8324
3483 940.9227 1879.8308
3484 941.4583 1880.9020
3485 941.4738 1880.9330
3486 941.4839 1880.9532
3487 630.3150 1887.9232
3488 945.4358 1888.8570
3490 945.9489 1889.8832
3492 631.6315 1891.8727
3493 473.9760 1891.8749
3494 947.4391 1892.8636
3496 947.9760 1893.9374
3497 948.4778 1894.9410
3498 948.4945 1894.9744
3499 632.9950 1895.9632
3500 633.2881 1896.8425
3501 949.4705 1896.9264
3502 949.9728 1897.9310
3503 951.4033 1900.7920
3506 635.6868 1904.0386
3507 477.0171 1904.0393
3508 953.4366 1904.8586
3509 953.4442 1904.8738
3511 954.4471 1906.8796
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