MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3401–3500 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3288 855.4175 1708.8204
3289 570.7191 1709.1355
3290 855.8615 1709.7084
3292 855.8748 1709.7350
3293 856.9048 1711.7950
3294 856.9050 1711.7954
3295 572.2418 1713.7036
3296 857.8673 1713.7200
3297 858.3975 1714.7804
3298 858.4078 1714.8010
3299 858.4164 1714.8182
3300 858.4211 1714.8276
3301 573.9493 1718.8261
3302 860.4207 1718.8268
3303 860.8954 1719.7762
3305 861.8990 1721.7834
3306 862.3754 1722.7362
3307 862.4064 1722.7982
3308 863.3679 1724.7212
3309 863.4120 1724.8094
3310 863.4125 1724.8104
3311 863.8612 1725.7078
3312 863.8640 1725.7134
3313 863.9286 1725.8426
3314 576.2966 1725.8680
3315 864.4332 1726.8518
3316 865.1841 1728.3536
3317 577.5657 1729.6753
3318 866.3511 1730.6876
3319 866.4255 1730.8364
3320 866.8559 1731.6972
3321 578.5660 1732.6762
3322 867.8934 1733.7722
3323 867.8970 1733.7794
3324 867.9039 1733.7932
3325 871.3687 1740.7228
3326 871.3821 1740.7496
3327 871.4164 1740.8182
3328 871.4256 1740.8366
3329 871.8613 1741.7080
3330 871.8682 1741.7218
3331 871.8684 1741.7222
3332 581.5819 1741.7239
3333 871.9074 1741.8002
3334 872.4269 1742.8392
3335 582.2408 1743.7006
3336 872.8576 1743.7006
3337 872.8688 1743.7230
3338 873.4211 1744.8276
3340 873.9386 1745.8626
3341 874.3374 1746.6602
3342 583.2278 1746.6616
3343 874.3390 1746.6634
3344 583.3065 1746.8977
3345 583.3069 1746.8989
3346 583.5613 1747.6621
3347 874.9210 1747.8274
3348 875.3915 1748.7684
3349 875.3961 1748.7776
3350 584.2697 1749.7873
3351 876.3972 1750.7798
3352 876.4008 1750.7870
3353 877.9542 1753.8938
3354 879.4532 1756.8918
3355 879.4675 1756.9204
3356 879.8641 1757.7136
3357 879.8809 1757.7472
3359 587.2441 1758.7105
3360 880.3653 1758.7160
3361 880.4229 1758.8312
3362 880.8578 1759.7010
3365 587.9588 1760.8546
3366 881.9238 1761.8330
3367 882.3215 1762.6284
3368 588.5515 1762.6327
3369 882.3959 1762.7772
3370 882.4238 1762.8330
3371 883.4179 1764.8212
3372 883.8959 1765.7772
3373 884.3724 1766.7302
3374 884.8680 1767.7214
3375 886.4282 1770.8418
3377 887.4310 1772.8474
3379 888.4192 1774.8238
3380 888.8619 1775.7092
3381 592.9563 1775.8471
3382 593.2845 1776.8317
3383 889.4417 1776.8688
3384 890.4147 1778.8148
3385 891.4362 1780.8578
3386 595.2736 1782.7990
3387 595.5649 1783.6729
3388 892.9346 1783.8546
3389 892.9363 1783.8580
3390 896.4108 1790.8070
3391 896.4153 1790.8160
3392 896.4165 1790.8184
3393 897.9229 1793.8312
3395 599.8945 1796.6617
3396 899.8361 1797.6576
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