MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3301–3400 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3168 811.8464 1621.6782
3169 811.8489 1621.6832
3170 811.9074 1621.8002
3171 812.3699 1622.7252
3172 812.3705 1622.7264
3173 812.9125 1623.8104
3174 542.9323 1625.7751
3176 814.3953 1626.7760
3177 814.3964 1626.7782
3178 814.4094 1626.8042
3179 814.8843 1627.7540
3181 814.9552 1627.8958
3183 544.2268 1629.6586
3185 816.4086 1630.8026
3186 816.8871 1631.7596
3187 816.9010 1631.7874
3189 816.9197 1631.8248
3190 817.2903 1632.5660
3191 817.4062 1632.7978
3194 818.3861 1634.7576
3196 818.9436 1635.8726
3197 818.9452 1635.8758
3198 819.3586 1636.7026
3199 820.3674 1638.7202
3200 547.5961 1639.7665
3201 547.5990 1639.7752
3202 821.3908 1640.7670
3203 821.9059 1641.7972
3204 822.3697 1642.7248
3205 548.8905 1643.6497
3206 822.8383 1643.6620
3207 822.8967 1643.7788
3208 823.3605 1644.7064
3209 549.2473 1644.7201
3210 549.5557 1645.6453
3211 824.4244 1646.8342
3213 824.9078 1647.8010
3214 825.8865 1649.7584
3215 825.9333 1649.8520
3216 826.4388 1650.8630
3217 827.4053 1652.7960
3218 827.4070 1652.7994
3219 827.4142 1652.8138
3220 552.6056 1654.7950
3222 552.9215 1655.7427
3223 829.9046 1657.7946
3224 554.2158 1659.6256
3225 554.2168 1659.6286
3226 830.8220 1659.6294
3227 831.3859 1660.7572
3228 554.6103 1660.8091
3230 832.4014 1662.7882
3233 834.3994 1666.7842
3234 834.8602 1667.7058
3235 834.9057 1667.7968
3236 834.9979 1667.9812
3237 834.9979 1667.9812
3238 557.0014 1667.9824
3239 557.3353 1668.9841
3240 836.9426 1671.8706
3241 836.9446 1671.8746
3242 837.9026 1673.7906
3243 559.2823 1674.8251
3244 838.4236 1674.8326
3245 838.8054 1675.5962
3246 559.5403 1675.5991
3247 839.3876 1676.7606
3248 839.3885 1676.7624
3249 840.3538 1678.6930
3250 840.4190 1678.8234
3251 840.8804 1679.7462
3252 841.9339 1681.8532
3253 842.4304 1682.8462
3255 842.9118 1683.8090
3256 843.3862 1684.7578
3257 843.3951 1684.7756
3258 843.3956 1684.7766
3259 843.3970 1684.7794
3260 843.4034 1684.7922
3261 843.4049 1684.7952
3263 845.4247 1688.8348
3264 845.9072 1689.7998
3267 846.4487 1690.8828
3268 846.8834 1691.7522
3269 847.3840 1692.7534
3271 847.8703 1693.7260
3272 847.8912 1693.7678
3273 847.9574 1693.9002
3274 849.8756 1697.7366
3275 850.4234 1698.8322
3276 567.5803 1699.7191
3277 852.3908 1702.7670
3278 568.6171 1702.8295
3279 568.6171 1702.8295
3282 853.4219 1704.8292
3283 853.4276 1704.8406
3284 853.9237 1705.8328
3285 854.3829 1706.7512
3286 854.8810 1707.7474
3287 855.3630 1708.7114
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