MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3201–3300 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3020 759.4059 1516.7972
3022 759.8803 1517.7460
3023 507.2028 1518.5866
3024 760.3015 1518.5884
3025 760.3655 1518.7164
3026 760.8589 1519.7032
3027 761.3381 1520.6616
3029 761.8474 1521.6802
3031 762.3552 1522.6958
3033 762.3904 1522.7662
3034 763.3610 1524.7074
3035 509.2599 1524.7579
3037 382.9208 1527.6541
3038 765.3767 1528.7388
3041 765.9099 1529.8052
3042 765.9107 1529.8068
3044 766.8832 1531.7518
3045 767.3604 1532.7062
3046 767.3889 1532.7632
3047 767.4034 1532.7922
3048 767.4039 1532.7932
3049 767.8981 1533.7816
3050 767.8987 1533.7828
3051 512.5355 1534.5847
3052 768.8701 1535.7256
3053 513.2598 1536.7576
3055 513.5789 1537.7149
3057 770.3529 1538.6912
3058 770.3607 1538.7068
3059 514.2325 1539.6757
3060 514.5897 1540.7473
3061 515.5483 1543.6231
3062 773.8157 1545.6168
3064 773.9059 1545.7972
3067 774.3727 1546.7308
3069 774.8551 1547.6956
3070 775.3665 1548.7184
3074 776.8964 1551.7782
3075 777.8582 1553.7018
3077 778.3840 1554.7534
3078 778.8363 1555.6580
3088 782.3712 1562.7278
3089 782.4117 1562.8088
3090 782.8635 1563.7124
3091 782.8705 1563.7264
3092 783.3715 1564.7284
3093 785.8831 1569.7516
3095 786.3694 1570.7242
3096 786.9100 1571.8054
3097 787.4213 1572.8280
3099 787.8971 1573.7796
3100 787.9014 1573.7882
3101 787.9193 1573.8240
3103 789.8968 1577.7790
3105 526.9346 1577.7820
3106 790.8893 1579.7640
3107 790.8895 1579.7644
3108 792.3755 1582.7364
3109 792.4193 1582.8240
3110 792.8667 1583.7188
3112 793.8611 1585.7076
3113 397.4422 1585.7397
3114 529.5872 1585.7398
3115 397.4427 1585.7417
3116 529.5884 1585.7434
3118 794.4265 1586.8384
3121 794.9198 1587.8250
3122 530.8828 1589.6266
3123 795.8213 1589.6280
3124 795.8869 1589.7592
3125 795.9112 1589.8078
3126 795.9122 1589.8098
3127 795.9138 1589.8130
3128 796.3734 1590.7322
3130 797.3882 1592.7618
3131 797.3892 1592.7638
3132 798.3684 1594.7222
3133 798.3763 1594.7380
3134 798.3779 1594.7412
3135 798.8749 1595.7352
3136 798.8862 1595.7578
3137 800.8676 1599.7206
3140 534.2510 1599.7312
3141 534.2512 1599.7318
3142 534.9474 1601.8204
3145 802.4111 1602.8076
3146 535.2834 1602.8284
3147 802.8453 1603.6760
3148 802.8827 1603.7508
3149 802.9145 1603.8144
3150 803.3699 1604.7252
3155 805.8699 1609.7252
3156 807.3627 1612.7108
3158 539.5732 1615.6978
3160 808.9032 1615.7918
3161 810.3986 1618.7826
3162 810.3996 1618.7846
3163 540.6073 1618.8001
3166 810.4164 1618.8182
3167 810.9085 1619.8024
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