MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3101–3200 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2885 715.3433 1428.6720
2886 715.8691 1429.7236
2887 716.3859 1430.7572
2888 716.3893 1430.7640
2889 716.8205 1431.6264
2890 716.8414 1431.6682
2891 717.3140 1432.6134
2895 718.3815 1434.7484
2896 719.3362 1436.6578
2897 719.3392 1436.6638
2900 720.8697 1439.7248
2901 721.3602 1440.7058
2902 721.8529 1441.6912
2903 722.3442 1442.6738
2904 722.3552 1442.6958
2905 482.2198 1443.6376
2906 722.8804 1443.7462
2909 482.9137 1445.7193
2911 723.8718 1445.7290
2912 723.8724 1445.7302
2913 723.8730 1445.7314
2914 723.8739 1445.7332
2915 723.8740 1445.7334
2916 723.8745 1445.7344
2917 723.8753 1445.7360
2918 723.8754 1445.7362
2919 723.8768 1445.7390
2921 724.3757 1446.7368
2922 724.3761 1446.7376
2926 725.3505 1448.6864
2927 725.3712 1448.7278
2928 725.8281 1449.6416
2929 726.3616 1450.7086
2930 726.3675 1450.7204
2931 726.3748 1450.7350
2932 728.3564 1454.6982
2933 728.3685 1454.7224
2935 728.4349 1454.8552
2936 728.4355 1454.8564
2937 728.8637 1455.7128
2938 486.8908 1457.6506
2941 730.8763 1459.7380
2942 730.8764 1459.7382
2943 731.8557 1461.6968
2944 731.8586 1461.7026
2945 731.8665 1461.7184
2946 732.3631 1462.7116
2947 732.8654 1463.7162
2949 733.8436 1465.6726
2950 735.3620 1468.7094
2951 736.3513 1470.6880
2953 738.9008 1475.7870
2954 739.3694 1476.7242
2955 739.3856 1476.7566
2956 739.7596 1477.5046
2957 739.8544 1477.6942
2958 739.8621 1477.7096
2959 739.8643 1477.7140
2961 741.3815 1480.7484
2962 741.8421 1481.6696
2963 741.8501 1481.6856
2964 495.5624 1483.6654
2965 742.8464 1483.6782
2966 742.8674 1483.7202
2967 495.5818 1483.7236
2968 742.8788 1483.7430
2969 495.9089 1484.7049
2971 743.3759 1484.7372
2972 743.8786 1485.7426
2973 745.3635 1488.7124
2974 745.8521 1489.6896
2976 497.5761 1489.7065
2977 745.8616 1489.7086
2978 745.8618 1489.7090
2979 746.3968 1490.7790
2980 746.8723 1491.7300
2981 747.8441 1493.6736
2982 748.3580 1494.7014
2983 748.8485 1495.6824
2984 748.8500 1495.6854
2986 749.8779 1497.7412
2990 500.9120 1499.7142
2992 500.9130 1499.7172
2994 751.8735 1501.7324
2995 752.3806 1502.7466
2996 752.8615 1503.7084
3001 753.8591 1505.7036
3002 754.3152 1506.6158
3003 754.3655 1506.7164
3007 755.3047 1508.5948
3008 755.3051 1508.5956
3009 755.3266 1508.6386
3010 756.8785 1511.7424
3011 756.8802 1511.7458
3012 757.3750 1512.7354
3013 757.3810 1512.7474
3014 505.5625 1513.6657
3017 758.4905 1514.9664
3018 758.8698 1515.7250
3019 759.3882 1516.7618
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