MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3001–3100 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2730 632.8207 1263.6268
2731 634.2585 1266.5024
2732 635.3273 1268.6400
2734 635.8320 1269.6494
2735 635.8490 1269.6834
2736 636.2980 1270.5814
2741 636.3235 1270.6324
2743 638.3219 1274.6292
2744 638.7952 1275.5758
2745 638.8350 1275.6554
2746 638.8370 1275.6594
2749 428.5648 1282.6726
2750 642.8088 1283.6030
2752 643.3358 1284.6570
2753 644.2968 1286.5790
2755 645.3228 1288.6310
2758 647.3271 1292.6396
2761 649.8223 1297.6300
2764 651.3310 1300.6474
2765 651.8436 1301.6726
2766 652.3409 1302.6672
2767 652.7736 1303.5326
2768 654.3919 1306.7692
2771 655.8183 1309.6220
2772 656.3344 1310.6542
2773 656.8337 1311.6528
2776 438.8748 1313.6026
2779 659.3273 1316.6400
2783 660.8276 1319.6406
2784 661.3018 1320.5890
2785 661.3474 1320.6802
2786 661.3490 1320.6834
2787 662.8416 1323.6686
2789 663.3040 1324.5934
2790 663.7979 1325.5812
2792 664.8334 1327.6522
2793 666.3542 1330.6938
2794 666.8323 1331.6500
2795 666.8327 1331.6508
2796 666.8491 1331.6836
2797 667.3439 1332.6732
2798 667.3469 1332.6792
2799 667.3776 1332.7406
2802 669.3263 1336.6380
2803 669.3483 1336.6820
2805 669.4027 1336.7908
2806 671.3582 1340.7018
2807 671.3630 1340.7114
2810 672.8288 1343.6430
2812 674.3318 1346.6490
2814 675.3940 1348.7734
2815 676.8782 1351.7418
2818 677.3447 1352.6748
2819 677.3447 1352.6748
2820 678.3339 1354.6532
2821 682.3522 1362.6898
2822 683.8347 1365.6548
2823 684.3305 1366.6464
2828 687.8766 1373.7386
2830 688.8453 1375.6760
2831 688.8464 1375.6782
2832 689.3381 1376.6616
2833 689.8608 1377.7070
2834 690.3298 1378.6450
2835 460.5566 1378.6480
2836 460.5568 1378.6486
2837 690.3518 1378.6890
2838 690.8845 1379.7544
2839 691.8138 1381.6130
2840 692.3411 1382.6676
2841 692.8538 1383.6930
2843 693.3433 1384.6720
2844 693.3476 1384.6806
2845 693.8207 1385.6268
2848 464.2367 1389.6883
2851 464.5457 1390.6153
2852 696.3336 1390.6526
2853 697.8587 1393.7028
2854 465.9105 1394.7097
2857 700.3724 1398.7302
2858 700.8687 1399.7228
2859 700.8693 1399.7240
2860 701.2899 1400.5652
2861 701.3397 1400.6648
2862 701.7848 1401.5550
2864 702.8625 1403.7104
2865 703.3536 1404.6926
2868 703.8558 1405.6970
2869 704.3325 1406.6504
2870 704.8661 1407.7176
2873 708.3925 1414.7704
2874 708.9113 1415.8080
2875 709.3248 1416.6350
2876 710.8593 1419.7040
2877 710.8602 1419.7058
2879 711.3976 1420.7806
2880 713.3417 1424.6688
2881 713.3546 1424.6946
2882 714.3248 1426.6350
2884 714.8430 1427.6714
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