MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2901–3000 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2592 1173.5543 1172.5470
2593 1174.5364 1173.5291
2594 1174.5447 1173.5374
2595 1174.5609 1173.5536
2596 1174.5636 1173.5563
2597 1175.5527 1174.5454
2598 588.2881 1174.5616
2600 1176.5342 1175.5269
2601 1176.5544 1175.5471
2602 1176.5565 1175.5492
2603 588.7821 1175.5496
2604 1177.5460 1176.5387
2605 1177.5603 1176.5530
2606 1178.5295 1177.5222
2607 1178.5577 1177.5504
2608 1178.5631 1177.5558
2609 1178.5728 1177.5655
2611 1179.5497 1178.5424
2613 1179.5629 1178.5556
2615 1180.5519 1179.5446
2616 1180.5590 1179.5517
2617 1180.5620 1179.5547
2618 591.2766 1180.5386
2619 1181.5524 1180.5451
2620 1181.5691 1180.5618
2621 1182.5520 1181.5447
2622 591.8132 1181.6118
2623 591.8135 1181.6124
2624 591.8140 1181.6134
2627 1183.5674 1182.5601
2628 1183.5691 1182.5618
2629 592.2943 1182.5740
2630 395.1988 1182.5746
2632 1184.5563 1183.5490
2633 1184.5610 1183.5537
2634 592.7877 1183.5608
2635 1184.5696 1183.5623
2636 592.7905 1183.5664
2637 1184.5759 1183.5686
2638 1186.5481 1185.5408
2639 1186.5652 1185.5579
2640 1187.5482 1186.5409
2641 1188.5482 1187.5409
2643 1188.6240 1187.6167
2645 1189.5574 1188.5501
2646 1189.5734 1188.5661
2647 1190.5614 1189.5541
2648 1190.5646 1189.5573
2649 1190.5712 1189.5639
2650 1191.5555 1190.5482
2651 1191.5708 1190.5635
2653 1193.5535 1192.5462
2654 597.7927 1193.5708
2655 1195.5519 1194.5446
2658 1196.5631 1195.5558
2659 1197.5470 1196.5397
2661 600.7839 1199.5532
2662 600.7841 1199.5536
2664 601.2974 1200.5802
2668 602.8092 1203.6038
2669 603.2967 1204.5788
2670 603.7925 1205.5704
2671 604.3054 1206.5962
2673 604.8187 1207.6228
2674 604.8214 1207.6282
2676 606.7889 1211.5632
2677 607.3464 1212.6782
2678 607.7877 1213.5608
2679 607.7884 1213.5622
2681 608.7825 1215.5504
2682 609.2966 1216.5786
2683 609.7754 1217.5362
2685 609.8104 1217.6062
2686 610.3062 1218.5978
2687 610.3245 1218.6344
2688 610.3254 1218.6362
2689 610.8204 1219.6262
2692 611.8093 1221.6040
2696 611.8306 1221.6466
2697 611.8361 1221.6576
2698 611.8370 1221.6594
2699 612.8601 1223.7056
2701 409.5253 1225.5541
2702 613.7845 1225.5544
2703 614.8163 1227.6180
2704 615.7849 1229.5552
2705 615.7855 1229.5564
2708 618.7789 1235.5432
2709 618.8157 1235.6168
2710 620.3253 1238.6360
2712 620.8154 1239.6162
2715 621.3745 1240.7344
2716 623.3379 1244.6612
2717 624.7820 1247.5494
2721 629.8011 1257.5876
2723 629.8479 1257.6812
2726 630.7907 1259.5668
2727 631.3196 1260.6246
2728 631.3330 1260.6514
2729 631.3349 1260.6552
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