MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2801–2900 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2477 1146.5486 1145.5413
2479 1147.5476 1146.5403
2481 1147.5555 1146.5482
2482 1148.5612 1147.5539
2483 1149.5250 1148.5177
2484 1149.5327 1148.5254
2485 1149.5347 1148.5274
2486 1149.5375 1148.5302
2488 1149.5453 1148.5380
2489 1149.5571 1148.5498
2490 575.2830 1148.5514
2491 575.2848 1148.5550
2493 1149.5690 1148.5617
2494 1150.5363 1149.5290
2495 1150.5443 1149.5370
2496 575.7771 1149.5396
2497 1150.5479 1149.5406
2499 1150.5549 1149.5476
2500 575.8713 1149.7280
2501 1151.5413 1150.5340
2502 1151.5439 1150.5366
2503 576.2775 1150.5404
2505 1151.5532 1150.5459
2506 576.3330 1150.6514
2508 1152.5491 1151.5418
2509 1153.5314 1152.5241
2510 1153.5481 1152.5408
2511 577.7504 1153.4862
2512 1154.5262 1153.5189
2513 1154.5349 1153.5276
2514 1154.5360 1153.5287
2515 1154.5382 1153.5309
2516 1154.5458 1153.5385
2518 577.7869 1153.5592
2519 385.5410 1153.6012
2520 577.8082 1153.6018
2521 1155.5459 1154.5386
2522 578.7668 1155.5190
2523 1156.5359 1155.5286
2524 578.7726 1155.5306
2525 1156.5426 1155.5353
2526 1156.5532 1155.5459
2527 1156.5565 1155.5492
2528 1157.5294 1156.5221
2529 1157.5424 1156.5351
2530 1158.5116 1157.5043
2531 579.7600 1157.5054
2532 1158.5552 1157.5479
2533 1158.5602 1157.5529
2534 1159.5396 1158.5323
2535 1159.5565 1158.5492
2536 1160.5272 1159.5199
2537 1160.5457 1159.5384
2538 1160.5543 1159.5470
2541 1161.5347 1160.5274
2542 1161.5424 1160.5351
2543 1161.5521 1160.5448
2544 1161.5551 1160.5478
2545 1162.5426 1161.5353
2546 1162.5449 1161.5376
2547 1162.5544 1161.5471
2548 581.8030 1161.5914
2549 581.8165 1161.6184
2550 581.8174 1161.6202
2551 1163.5415 1162.5342
2552 582.2990 1162.5834
2553 1164.5488 1163.5415
2554 1164.5571 1163.5498
2555 1164.5571 1163.5498
2556 388.8703 1163.5891
2557 582.8090 1163.6034
2559 582.8118 1163.6090
2560 1165.5436 1164.5363
2563 1166.5360 1165.5287
2564 1166.5448 1165.5375
2565 583.7773 1165.5400
2566 1166.5576 1165.5503
2567 1167.5361 1166.5288
2568 1167.5492 1166.5419
2569 1167.5581 1166.5508
2570 584.3123 1166.6100
2571 1168.5524 1167.5451
2572 1168.5554 1167.5481
2573 1169.5283 1168.5210
2574 1169.5358 1168.5285
2575 1169.5535 1168.5462
2576 1169.5590 1168.5517
2577 1169.5670 1168.5597
2578 1170.5513 1169.5440
2579 1170.5552 1169.5479
2580 585.7822 1169.5498
2581 1170.5696 1169.5623
2582 1170.5698 1169.5625
2585 586.2841 1170.5536
2586 1172.5386 1171.5313
2587 1172.5536 1171.5463
2588 1172.5549 1171.5476
2589 1173.5417 1172.5344
2590 1173.5417 1172.5344
2591 1173.5465 1172.5392
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