MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2601–2700 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2240 1084.5254 1083.5181
2242 1085.5098 1084.5025
2243 1085.5247 1084.5174
2244 1085.5355 1084.5282
2245 1085.5557 1084.5484
2246 1086.5085 1085.5012
2247 1086.5187 1085.5114
2248 1088.5115 1087.5042
2252 1089.5082 1088.5009
2253 1089.5150 1088.5077
2254 1089.5208 1088.5135
2255 545.2755 1088.5364
2257 364.1653 1089.4741
2258 1090.5046 1089.4973
2259 1090.5195 1089.5122
2260 1090.5237 1089.5164
2261 1090.5238 1089.5165
2262 1090.5354 1089.5281
2263 1090.5385 1089.5312
2264 545.8021 1089.5896
2265 1091.5056 1090.4983
2266 1091.5325 1090.5252
2267 1091.5338 1090.5265
2268 1091.5391 1090.5318
2269 546.2806 1090.5466
2270 1092.5007 1091.4934
2272 1092.5396 1091.5323
2275 1093.5123 1092.5050
2276 1093.5129 1092.5056
2277 1093.5194 1092.5121
2278 1093.5242 1092.5169
2279 1093.5380 1092.5307
2280 1094.5178 1093.5105
2281 1094.5293 1093.5220
2282 1094.5337 1093.5264
2283 1095.5209 1094.5136
2284 548.7509 1095.4872
2285 1096.5271 1095.5198
2286 1096.5415 1095.5342
2287 1097.5065 1096.4992
2288 1097.5245 1096.5172
2289 1097.5320 1096.5247
2290 1098.5038 1097.4965
2291 1098.5103 1097.5030
2292 549.7638 1097.5130
2293 1099.5284 1098.5211
2295 1100.5105 1099.5032
2296 1100.5155 1099.5082
2297 550.7842 1099.5538
2298 1101.5386 1100.5313
2299 1102.5078 1101.5005
2300 1102.5195 1101.5122
2302 1103.5072 1102.4999
2304 1103.5132 1102.5059
2305 1103.5192 1102.5119
2306 1103.5250 1102.5177
2307 1103.5300 1102.5227
2310 368.5361 1102.5865
2311 1104.5154 1103.5081
2312 1104.5209 1103.5136
2313 1104.5376 1103.5303
2314 1105.5204 1104.5131
2316 1105.5289 1104.5216
2317 1105.5317 1104.5244
2318 553.2885 1104.5624
2321 553.3051 1104.5956
2323 1106.5243 1105.5170
2325 553.7977 1105.5808
2326 1107.5148 1106.5075
2327 1107.5270 1106.5197
2328 1107.5903 1106.5830
2329 1108.5153 1107.5080
2330 1108.5190 1107.5117
2331 1108.5271 1107.5198
2332 1108.5292 1107.5219
2333 1108.5294 1107.5221
2334 1109.5270 1108.5197
2335 1109.5398 1108.5325
2336 1110.5288 1109.5215
2337 1110.5314 1109.5241
2338 1110.5491 1109.5418
2339 1111.5137 1110.5064
2340 1111.5248 1110.5175
2341 1111.5284 1110.5211
2342 1111.5348 1110.5275
2343 556.3142 1110.6138
2344 1112.5258 1111.5185
2345 1113.5111 1112.5038
2346 1113.5186 1112.5113
2347 1113.5284 1112.5211
2348 1113.5303 1112.5230
2349 557.2816 1112.5486
2350 557.3087 1112.6028
2351 557.3091 1112.6036
2352 1114.5128 1113.5055
2353 557.7619 1113.5092
2354 1114.5200 1113.5127
2355 1114.5320 1113.5247
2356 558.2622 1114.5098
2357 1115.5172 1114.5099
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