MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2501–2600 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2123 1052.4987 1051.4914
2124 1052.5032 1051.4959
2125 1052.5132 1051.5059
2126 1053.4972 1052.4899
2127 1053.4974 1052.4901
2128 1053.5078 1052.5005
2129 1053.5245 1052.5172
2130 527.2905 1052.5664
2131 527.2914 1052.5682
2134 1054.4933 1053.4860
2135 1054.4999 1053.4926
2136 1054.5114 1053.5041
2137 1055.5049 1054.4976
2138 528.2947 1054.5748
2139 1056.5038 1055.4965
2140 1056.5215 1055.5142
2142 1057.5009 1056.4936
2143 1057.5116 1056.5043
2144 529.7507 1057.4868
2146 1058.5055 1057.4982
2148 1059.4935 1058.4862
2149 1059.4985 1058.4912
2150 1059.5051 1058.4978
2154 1060.5095 1059.5022
2155 530.7962 1059.5778
2156 1061.4934 1060.4861
2157 1061.5056 1060.4983
2158 1062.4730 1061.4657
2159 1062.4972 1061.4899
2160 1062.5065 1061.4992
2161 1062.5106 1061.5033
2162 1062.5118 1061.5045
2163 1062.5140 1061.5067
2164 1063.4745 1062.4672
2165 532.2690 1062.5234
2166 532.3005 1062.5864
2167 1064.4875 1063.4802
2168 1064.5011 1063.4938
2169 1064.5054 1063.4981
2170 532.7744 1063.5342
2171 1064.5436 1063.5363
2172 1065.5066 1064.4993
2174 1066.5010 1065.4937
2175 1066.5059 1065.4986
2176 1066.5137 1065.5064
2177 533.7624 1065.5102
2178 533.7639 1065.5132
2179 1067.5027 1066.4954
2180 1067.5200 1066.5127
2181 534.2695 1066.5244
2182 1068.4995 1067.4922
2183 1068.5106 1067.5033
2184 1068.5126 1067.5053
2185 1068.5166 1067.5093
2186 1069.0151 1068.0078
2187 1069.4758 1068.4685
2188 1069.4949 1068.4876
2189 1069.5068 1068.4995
2191 1069.5229 1068.5156
2192 1071.5071 1070.4998
2193 1071.5245 1070.5172
2194 1072.5256 1071.5183
2195 536.7683 1071.5220
2196 1073.4937 1072.4864
2197 537.2530 1072.4914
2198 1073.5088 1072.5015
2199 1073.5131 1072.5058
2200 1073.5226 1072.5153
2201 537.7482 1073.4818
2202 1074.5161 1073.5088
2204 1075.5140 1074.5067
2205 1075.5188 1074.5115
2207 1076.5143 1075.5070
2208 1076.5157 1075.5084
2209 538.7756 1075.5366
2210 359.5631 1075.6675
2211 1077.1761 1076.1688
2212 1077.5004 1076.4931
2213 1077.5160 1076.5087
2214 1078.4995 1077.4922
2217 539.8428 1077.6710
2218 1079.5204 1078.5131
2219 540.2657 1078.5168
2220 1079.5372 1078.5299
2221 1080.5177 1079.5104
2222 1080.5287 1079.5214
2224 1080.5432 1079.5359
2225 1080.5615 1079.5542
2227 541.2592 1080.5038
2228 1081.5118 1080.5045
2229 1081.5239 1080.5166
2230 1082.5018 1081.4945
2231 1082.5138 1081.5065
2232 1082.5144 1081.5071
2233 1083.5045 1082.4972
2234 1083.5062 1082.4989
2235 1083.5161 1082.5088
2236 1083.5168 1082.5095
2238 542.7590 1083.5034
2239 1084.5221 1083.5148
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