MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2401–2500 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2001 1023.4893 1022.4820
2002 1023.4899 1022.4826
2004 1023.4981 1022.4908
2005 512.2849 1022.5552
2006 1024.4821 1023.4748
2007 512.7448 1023.4750
2008 1024.4824 1023.4751
2009 1024.4940 1023.4867
2010 1024.5016 1023.4943
2012 1025.4845 1024.4772
2013 1025.4861 1024.4788
2014 1026.4648 1025.4575
2015 1026.4708 1025.4635
2016 1026.4812 1025.4739
2017 1026.4912 1025.4839
2019 1027.4850 1026.4777
2020 1027.4929 1026.4856
2021 1027.4990 1026.4917
2023 514.2758 1026.5370
2024 1029.4808 1028.4735
2025 1029.4918 1028.4845
2026 1029.5062 1028.4989
2027 1030.4883 1029.4810
2028 1030.4933 1029.4860
2029 1030.4940 1029.4867
2030 1030.5007 1029.4934
2031 1031.4806 1030.4733
2032 1031.4857 1030.4784
2033 1031.4904 1030.4831
2036 516.2873 1030.5600
2037 1032.4856 1031.4783
2038 1032.5035 1031.4962
2039 516.7661 1031.5176
2040 516.7839 1031.5532
2041 1033.4827 1032.4754
2042 1033.4954 1032.4881
2043 1033.5007 1032.4934
2044 517.2715 1032.5284
2045 1034.5028 1033.4955
2047 345.8069 1034.3989
2048 518.2068 1034.3990
2049 1035.4663 1034.4590
2051 1035.4790 1034.4717
2052 1035.4846 1034.4773
2053 1035.4974 1034.4901
2054 518.2610 1034.5074
2055 1035.5166 1034.5093
2057 518.2849 1034.5552
2058 1036.4977 1035.4904
2059 1037.5006 1036.4933
2060 1037.5059 1036.4986
2061 1037.5103 1036.5030
2063 1038.5012 1037.4939
2064 1038.5026 1037.4953
2065 1038.5027 1037.4954
2066 1039.4801 1038.4728
2067 1039.4961 1038.4888
2068 1039.4965 1038.4892
2069 1039.5054 1038.4981
2070 1040.4872 1039.4799
2071 1040.4943 1039.4870
2072 1040.5028 1039.4955
2073 1040.5072 1039.4999
2074 1041.4957 1040.4884
2075 1041.4966 1040.4893
2076 1041.4988 1040.4915
2077 1042.4817 1041.4744
2078 1042.5181 1041.5108
2080 521.7637 1041.5128
2081 1043.4944 1042.4871
2084 1044.4852 1043.4779
2085 1044.4962 1043.4889
2086 1044.5038 1043.4965
2088 1046.4836 1045.4763
2089 523.7473 1045.4800
2090 1046.4948 1045.4875
2091 1047.4962 1046.4889
2092 1047.5024 1046.4951
2093 1047.5061 1046.4988
2094 1047.5161 1046.5088
2095 1048.4556 1047.4483
2096 1048.4718 1047.4645
2097 1048.4850 1047.4777
2098 1048.4877 1047.4804
2099 524.7551 1047.4956
2100 1048.5063 1047.4990
2101 1048.5111 1047.5038
2103 524.7780 1047.5414
2104 1048.5491 1047.5418
2108 1049.4968 1048.4895
2110 1049.5337 1048.5264
2112 1049.5693 1048.5620
2113 1049.5698 1048.5625
2114 525.2886 1048.5626
2115 525.2886 1048.5626
2118 1050.4874 1049.4801
2119 1050.5071 1049.4998
2120 1050.5685 1049.5612
2121 1051.4819 1050.4746
2122 526.2457 1050.4768
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