MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2301–2400 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1876 990.4604 989.4531
1877 990.4651 989.4578
1878 495.7411 989.4676
1879 496.2125 990.4104
1880 991.4581 990.4508
1881 991.4607 990.4534
1882 991.4814 990.4741
1883 496.2662 990.5178
1884 991.5255 990.5182
1885 992.4700 991.4627
1886 992.4753 991.4680
1887 496.7433 991.4720
1888 496.7457 991.4768
1889 496.7630 991.5114
1892 496.7788 991.5430
1893 993.4659 992.4586
1894 993.4678 992.4605
1895 993.4701 992.4628
1896 497.7329 993.4512
1897 994.4607 993.4534
1898 994.4696 993.4623
1899 994.4863 993.4790
1900 995.4648 994.4575
1901 995.4776 994.4703
1903 996.4674 995.4601
1904 996.4744 995.4671
1905 996.4775 995.4702
1907 996.4875 995.4802
1908 499.2335 996.4524
1911 499.2603 996.5060
1913 998.4567 997.4494
1914 998.4668 997.4595
1916 999.4788 998.4715
1918 500.2973 998.5800
1919 1000.1644 999.1571
1920 1000.4714 999.4641
1921 1000.4726 999.4653
1922 1000.4754 999.4681
1925 1001.4716 1000.4643
1926 1001.4786 1000.4713
1927 1001.4823 1000.4750
1928 1001.4848 1000.4775
1930 1002.4879 1001.4806
1931 1002.5151 1001.5078
1932 501.7626 1001.5106
1933 501.7631 1001.5116
1934 1002.5276 1001.5203
1935 1003.4695 1002.4622
1936 1004.4662 1003.4589
1937 1004.4748 1003.4675
1938 1004.4818 1003.4745
1940 1006.4888 1005.4815
1942 1007.4675 1006.4602
1943 504.2490 1006.4834
1944 1007.5195 1006.5122
1946 504.2651 1006.5156
1947 1007.5231 1006.5158
1948 504.7269 1007.4392
1949 1008.4860 1007.4787
1950 504.7469 1007.4792
1951 1008.5106 1007.5033
1952 1009.1691 1008.1618
1953 1009.4530 1008.4457
1954 1009.4738 1008.4665
1955 1009.4754 1008.4681
1956 1009.4789 1008.4716
1957 1009.4915 1008.4842
1958 1009.5026 1008.4953
1961 1010.5576 1009.5503
1963 1011.4857 1010.4784
1965 1011.5731 1010.5658
1966 1012.4709 1011.4636
1967 1013.4767 1012.4694
1968 1013.5022 1012.4949
1969 1014.4680 1013.4607
1970 1014.4893 1013.4820
1971 1014.5063 1013.4990
1972 1015.4758 1014.4685
1973 1015.4877 1014.4804
1974 1015.4891 1014.4818
1975 508.2579 1014.5012
1977 1016.4788 1015.4715
1978 1016.4793 1015.4720
1979 1016.4830 1015.4757
1982 1018.4835 1017.4762
1983 1018.4941 1017.4868
1984 510.2244 1018.4342
1985 1019.4749 1018.4676
1986 1019.4778 1018.4705
1987 1019.4833 1018.4760
1988 1019.4886 1018.4813
1989 1019.5010 1018.4937
1990 510.2739 1018.5332
1991 1020.4802 1019.4729
1992 1020.4805 1019.4732
1993 1020.4835 1019.4762
1994 510.7575 1019.5004
1995 511.2119 1020.4092
1996 511.2531 1020.4916
1997 1022.4799 1021.4726
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