MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2201–2300 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1746 947.4649 946.4576
1748 948.4927 947.4854
1749 949.4495 948.4422
1750 949.4523 948.4450
1751 949.4593 948.4520
1752 475.2973 948.5800
1754 475.7401 949.4656
1756 476.2166 950.4186
1758 951.4498 950.4425
1759 952.4531 951.4458
1760 952.4545 951.4472
1761 952.4579 951.4506
1763 477.2205 952.4264
1764 953.4397 952.4324
1765 953.4520 952.4447
1766 953.4527 952.4454
1767 953.4532 952.4459
1768 954.4492 953.4419
1769 954.4501 953.4428
1770 954.4576 953.4503
1771 954.4582 953.4509
1773 955.4543 954.4470
1774 955.4559 954.4486
1776 478.8069 955.5992
1777 957.4597 956.4524
1778 957.4891 956.4818
1779 958.4529 957.4456
1780 958.4562 957.4489
1781 959.4326 958.4253
1782 959.4641 958.4568
1783 960.4584 959.4511
1784 960.4626 959.4553
1785 960.5276 959.5203
1786 480.7679 959.5212
1787 961.4515 960.4442
1788 961.4537 960.4464
1789 961.4620 960.4547
1791 321.2429 960.7069
1792 962.4563 961.4490
1793 963.4645 962.4572
1797 482.2493 962.4840
1798 964.7661 963.7588
1799 965.4586 964.4513
1801 483.2580 964.5014
1802 966.4604 965.4531
1803 966.4658 965.4585
1804 966.4659 965.4586
1805 483.7366 965.4586
1807 484.2138 966.4130
1808 484.2476 966.4806
1810 968.4678 967.4605
1811 968.4679 967.4606
1812 484.7448 967.4750
1813 485.7460 969.4774
1815 971.4681 970.4608
1818 972.4656 971.4583
1820 973.4705 972.4632
1821 973.4765 972.4692
1823 973.4962 972.4889
1825 974.4632 973.4559
1826 975.4460 974.4387
1827 975.4769 974.4696
1829 976.4499 975.4426
1830 488.7659 975.5172
1831 977.5656 976.5583
1832 489.2870 976.5594
1833 978.4735 977.4662
1834 489.7675 977.5204
1835 979.4491 978.4418
1836 490.2289 978.4432
1837 979.4585 978.4512
1838 979.4699 978.4626
1839 490.3076 978.6006
1841 980.4572 979.4499
1842 980.4619 979.4546
1843 980.4645 979.4572
1844 980.4649 979.4576
1845 980.4674 979.4601
1846 980.4690 979.4617
1847 980.4747 979.4674
1848 980.4819 979.4746
1849 491.2373 980.4600
1850 981.4739 980.4666
1852 982.4838 981.4765
1854 983.4704 982.4631
1855 983.4885 982.4812
1856 984.4627 983.4554
1859 984.4687 983.4614
1860 984.4697 983.4624
1862 985.4630 984.4557
1863 985.4890 984.4817
1866 986.4590 985.4517
1867 986.4808 985.4735
1868 988.4576 987.4503
1869 988.4644 987.4571
1870 988.4684 987.4611
1871 988.4775 987.4702
1872 988.4781 987.4708
1873 988.4799 987.4726
1875 495.2758 988.5370
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