MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2001–2100 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1493 855.4263 854.4190
1495 428.2573 854.5000
1496 856.4001 855.3928
1497 857.4023 856.3950
1499 858.4116 857.4043
1500 859.3775 858.3702
1503 861.4131 860.4058
1505 861.4233 860.4160
1506 862.4031 861.3958
1507 862.4150 861.4077
1508 862.4438 861.4365
1509 862.6912 861.6839
1510 862.9031 861.8958
1511 432.1905 862.3664
1512 863.4185 862.4112
1513 863.4562 862.4489
1514 432.2412 862.4678
1516 866.4095 865.4022
1517 434.1959 866.3772
1518 867.3850 866.3777
1519 867.3854 866.3781
1520 868.4059 867.3986
1521 435.2274 868.4402
1522 869.4733 868.4660
1523 869.4792 868.4719
1525 436.2090 870.4034
1526 871.4133 870.4060
1527 872.4382 871.4309
1529 874.4303 873.4230
1530 876.3996 875.3923
1532 438.7399 875.4652
1533 877.4250 876.4177
1534 877.4260 876.4187
1535 878.4220 877.4147
1536 878.4315 877.4242
1538 439.7289 877.4432
1539 879.4162 878.4089
1540 440.2339 878.4532
1545 440.2369 878.4592
1547 440.2574 878.5002
1548 880.4176 879.4103
1549 880.4236 879.4163
1550 440.7495 879.4844
1551 441.7022 881.3898
1552 882.3995 881.3922
1553 882.4216 881.4143
1554 882.4267 881.4194
1555 882.4698 881.4625
1557 883.3823 882.3750
1558 883.4208 882.4135
1560 884.3967 883.3894
1562 443.2243 884.4340
1563 886.4332 885.4259
1564 886.4348 885.4275
1566 444.6814 887.3482
1567 888.5163 887.5090
1569 889.4365 888.4292
1571 890.4333 889.4260
1574 446.1753 890.3360
1575 891.4442 890.4369
1578 447.2657 892.5168
1579 894.4233 893.4160
1580 894.4550 893.4477
1581 895.3986 894.3913
1582 448.2035 894.3924
1583 895.4198 894.4125
1584 895.4343 894.4270
1586 448.7464 895.4782
1587 897.4330 896.4257
1589 449.6996 897.3846
1590 899.3758 898.3685
1591 899.4313 898.4240
1592 450.2350 898.4554
1593 450.2527 898.4908
1594 450.7253 899.4360
1595 901.4152 900.4079
1596 901.4319 900.4246
1597 902.4331 901.4258
1598 903.4317 902.4244
1599 903.4513 902.4440
1602 904.4395 903.4322
1604 453.1697 904.3248
1605 905.4767 904.4694
1606 453.2420 904.4694
1607 905.4769 904.4696
1609 906.4230 905.4157
1610 453.7198 905.4250
1613 907.4240 906.4167
1614 907.4388 906.4315
1615 907.4463 906.4390
1617 909.4393 908.4320
1618 909.4426 908.4353
1619 909.4438 908.4365
1620 910.4697 909.4624
1621 455.7417 909.4688
1622 911.3922 910.3849
1623 456.2002 910.3858
1624 911.4357 910.4284
1627 911.4594 910.4521
1628 912.4332 911.4259
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