MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1901–2000 (out of 4700)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1334 782.4053 781.3980
1335 392.1860 782.3574
1336 392.2225 782.4304
1337 392.2228 782.4310
1340 784.3754 783.3681
1341 392.7237 783.4328
1342 392.7252 783.4358
1343 784.5046 783.4973
1344 392.7560 783.4974
1345 784.5054 783.4981
1346 392.7566 783.4986
1347 785.3780 784.3707
1348 785.3867 784.3794
1349 393.2443 784.4740
1350 786.4719 785.4646
1354 395.2232 788.4318
1356 793.3689 792.3616
1357 397.2719 792.5292
1362 398.2101 794.4056
1364 398.7198 795.4250
1366 797.8899 796.8826
1368 799.4156 798.4083
1370 800.3824 799.3751
1380 803.3652 802.3579
1381 805.3796 804.3723
1382 805.3925 804.3852
1383 403.2192 804.4238
1385 806.3718 805.3645
1386 807.4343 806.4270
1387 810.3636 809.3563
1390 810.4556 809.4483
1391 811.4005 810.3932
1392 811.4078 810.4005
1395 812.3810 811.3737
1396 812.5352 811.5279
1397 813.4423 812.4350
1398 814.3960 813.3887
1399 814.4424 813.4351
1401 409.6671 817.3196
1402 818.4078 817.4005
1403 818.4088 817.4015
1404 410.7104 819.4062
1407 411.2179 820.4212
1408 411.2361 820.4576
1409 412.6727 823.3308
1410 824.3939 823.3866
1411 824.4985 823.4912
1412 824.9223 823.9150
1413 825.3730 824.3657
1414 825.3749 824.3676
1416 825.4327 824.4254
1417 825.8771 824.8698
1418 826.4007 825.3934
1421 827.4081 826.4008
1422 414.2185 826.4224
1424 828.3745 827.3672
1425 828.3749 827.3676
1426 828.4449 827.4376
1427 831.3849 830.3776
1429 416.6619 831.3092
1430 832.3992 831.3919
1431 832.4009 831.3936
1432 833.3923 832.3850
1433 417.2001 832.3856
1437 833.8991 832.8918
1438 834.4029 833.3956
1439 835.3965 834.3892
1442 418.7229 835.4312
1443 837.3891 836.3818
1444 839.3845 838.3772
1446 839.3930 838.3857
1447 840.4079 839.4006
1448 841.3698 840.3625
1449 841.3699 840.3626
1450 421.1893 840.3640
1452 841.3913 840.3840
1455 842.4006 841.3933
1456 842.4054 841.3981
1458 421.7256 841.4366
1459 842.4665 841.4592
1462 842.8867 841.8794
1463 422.2131 842.4116
1465 844.3876 843.3803
1466 844.4294 843.4221
1473 847.9114 846.9041
1475 848.3976 847.3903
1477 848.4152 847.4079
1478 849.4062 848.3989
1479 425.2915 848.5684
1480 850.3781 849.3708
1481 850.3856 849.3783
1482 850.4114 849.4041
1483 425.7618 849.5090
1484 851.3903 850.3830
1485 426.2360 850.4574
1486 853.4254 852.4181
1488 427.2494 852.4842
1490 854.4645 853.4572
1491 855.3870 854.3797
1492 855.4137 854.4064
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