MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 1 |
| MS data file | : | PRT1245_Other_P1_B9.mgf |
| Database | : | S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues) |
| Timestamp | : | 24 Jun 2025 at 21:21:15 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,728 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 3004 |
754.9098 | 1507.8050 | 1507.8107 | -3.72 | 1 | 8 | 0.95 | 1Score > 20 indicates identity |
LSVANVNEHQRIK + Deamidated (NQ) |
| 1379 |
401.7256 | 801.4366 | 801.4344 | 2.75 | 1 | 8 | 0.31 | 1Score > 24 indicates identity Score > 15 indicates homology |
GQGLNKGK + Deamidated (NQ) |
| 1643 |
919.4385 | 918.4312 | 918.4294 | 1.99 | 1 | 8 | 0.3 | 1Score > 22 indicates identity Score > 15 indicates homology |
EADEKEAK |
| 2817 |
451.8985 | 1352.6737 | 1352.6725 | 0.90 | 0 | 8 | 1 | 1Score > 22 indicates identity Score > 20 indicates homology |
VANGNNVVQFYK + Deamidated (NQ) |
| 2759 |
648.8340 | 1295.6534 | 1295.6721 | -14.4 | 0 | 8 | 0.8 | 1Score > 22 indicates identity Score > 19 indicates homology |
SNDDVLPPLAVR + Deamidated (NQ) |
| 2751 |
643.3063 | 1284.5980 | 1284.5808 | 13.4 | 0 | 8 | 0.32 | 1Score > 21 indicates identity Score > 15 indicates homology |
MEALYQTAWR + Deamidated (NQ); Oxidation (M) |
| 1061 |
616.3698 | 615.3625 | 615.3592 | 5.47 | 0 | 8 | 1 | 1Score > 25 indicates identity Score > 20 indicates homology |
AVIEGK |
| 2644 |
594.8165 | 1187.6184 | 1187.6299 | -9.62 | 0 | 8 | 1 | 1Score > 22 indicates identity Score > 20 indicates homology |
WQNTISNVVK |
| 1084 |
319.1877 | 636.3608 | 636.3707 | -15.5 | 0 | 8 | 0.25 | 1Score > 20 indicates identity Score > 14 indicates homology |
QHKPK |
| 1258 |
367.1857 | 732.3568 | 732.3442 | 17.2 | 1 | 8 | 0.59 | 1Score > 22 indicates identity Score > 18 indicates homology |
LGWDKN + Deamidated (NQ) |
| 2893 |
717.3481 | 1432.6816 | 1432.6809 | 0.51 | 1 | 8 | 0.41 | 1Score > 22 indicates identity Score > 16 indicates homology |
AVMFDRAFDSFK |
| 4104 |
790.0192 | 2367.0358 | 2367.0761 | -17.0 | 1 | 8 | 0.89 | 1Score > 20 indicates identity |
MSDHSPISNKENHLLPSDSSR + Deamidated (NQ); Oxidation (M) |
| 2849 |
695.8738 | 1389.7330 | 1389.7252 | 5.66 | 1 | 8 | 1 | 1Score > 21 indicates identity Score > 20 indicates homology |
IRELLNFNDEK |
| 2422 |
567.2902 | 1132.5658 | 1132.5798 | -12.3 | 1 | 8 | 0.61 | 1Score > 23 indicates identity Score > 18 indicates homology |
LDPKTAETMK |
| 1910 |
997.4600 | 996.4527 | 996.4552 | -2.53 | 0 | 7 | 0.67 | 1Score > 20 indicates identity Score > 18 indicates homology |
VSSWENFK + Deamidated (NQ) |
| 1960 |
505.2730 | 1008.5314 | 1008.5314 | 0.060 | 1 | 7 | 1 | 1Score > 21 indicates identity Score > 20 indicates homology |
MVDKIFEK |
| 2504 |
1151.5509 | 1150.5436 | 1150.5288 | 12.9 | 1 | 7 | 1 | 1Score > 21 indicates identity Score > 20 indicates homology |
KNSNMDIGQK + Deamidated (NQ); Oxidation (M) |
| 2599 |
588.2999 | 1174.5852 | 1174.5805 | 4.06 | 0 | 7 | 0.87 | 1Score > 23 indicates identity Score > 19 indicates homology |
MQAVFTNVHK + Deamidated (NQ) |
| 1718 |
470.2388 | 938.4630 | 938.4570 | 6.48 | 0 | 7 | 0.46 | 1Score > 20 indicates identity Score > 17 indicates homology |
QENLHNGK |
| 1542 |
879.4610 | 878.4537 | 878.4538 | -0.082 | 0 | 7 | 0.73 | 1Score > 22 indicates identity Score > 18 indicates homology |
YFPDLPK |
| 3087 |
781.9077 | 1561.8008 | 1561.8239 | -14.8 | 0 | 7 | 1 | 1Score > 23 indicates identity Score > 20 indicates homology |
ATLSTLFQELNVPK + 2 Deamidated (NQ) |
| 1145 |
336.2136 | 670.4126 | 670.4013 | 16.9 | 0 | 7 | 0.77 | 1Score > 19 indicates identity |
AIKPNK + Deamidated (NQ) |
| 2706 |
617.2935 | 1232.5724 | 1232.5893 | -13.7 | 0 | 7 | 1 | 1Score > 21 indicates identity Score > 20 indicates homology |
NQPGLSMMLNK + Deamidated (NQ) |
| 2778 |
658.8492 | 1315.6838 | 1315.6619 | 16.7 | 1 | 7 | 1 | 1Score > 22 indicates identity Score > 20 indicates homology |
LLGDNSPSKEQK + Deamidated (NQ) |
| 1460 |
421.7382 | 841.4618 | 841.4657 | -4.63 | 1 | 7 | 0.79 | 1Score > 19 indicates identity |
KNPAVGEK |
| 1976 |
508.2662 | 1014.5178 | 1014.5094 | 8.33 | 0 | 7 | 0.43 | 1Score > 21 indicates identity Score > 16 indicates homology |
GLNNGENVAK |
| 1717 |
939.4657 | 938.4584 | 938.4570 | 1.55 | 0 | 7 | 0.72 | 1Score > 20 indicates identity Score > 18 indicates homology |
QENLHNGK |
| 1962 |
506.2238 | 1010.4330 | 1010.4305 | 2.54 | 0 | 7 | 0.78 | 1Score > 19 indicates identity |
EQQQHDPK + 2 Deamidated (NQ) |
| 2056 |
518.2841 | 1034.5536 | 1034.5396 | 13.5 | 0 | 7 | 1 | 1Score > 21 indicates identity Score > 20 indicates homology |
VYSINNTPK |
| 2910 |
723.8680 | 1445.7214 | 1445.7085 | 8.94 | 0 | 7 | 1 | 1Score > 22 indicates identity Score > 20 indicates homology |
SHGAVLPSYCSLR |
| 1283 |
751.3754 | 750.3681 | 750.3773 | -12.2 | 0 | 7 | 0.79 | 1Score > 22 indicates identity Score > 19 indicates homology |
QNHPQK |
| 1890 |
992.5450 | 991.5377 | 991.5233 | 14.6 | 1 | 7 | 1 | 1Score > 22 indicates identity Score > 20 indicates homology |
MALSRSVGR + Oxidation (M) |
| 3606 |
992.9860 | 1983.9574 | 1983.9796 | -11.2 | 1 | 7 | 0.45 | 1Score > 22 indicates identity Score > 16 indicates homology |
DVRDILHCTNSGLNLTR + Deamidated (NQ) |
| 1060 |
308.6877 | 615.3608 | 615.3592 | 2.74 | 0 | 7 | 0.39 | 1Score > 25 indicates identity Score > 15 indicates homology |
AVIEGK |
| 2774 |
656.8367 | 1311.6588 | 1311.6744 | -11.9 | 1 | 7 | 1 | 1Score > 22 indicates identity Score > 19 indicates homology |
DVKSQISMIFK + Deamidated (NQ); Oxidation (M) |
| 2748 |
640.8197 | 1279.6248 | 1279.6329 | -6.32 | 1 | 7 | 1 | 1Score > 23 indicates identity Score > 19 indicates homology |
KTSQLMEEIGK + Deamidated (NQ); Oxidation (M) |
| 1127 |
661.3867 | 660.3794 | 660.3694 | 15.2 | 0 | 7 | 1 | 1Score > 22 indicates identity Score > 19 indicates homology |
TVLNTL + Deamidated (NQ) |
| 2301 |
552.2564 | 1102.4982 | 1102.5142 | -14.5 | 0 | 7 | 0.89 | 1Score > 19 indicates identity |
EGGETQEKPK + Deamidated (NQ) |
| 986 |
555.3143 | 554.3070 | 554.3176 | -19.2 | 0 | 7 | 0.34 | 1Score > 15 indicates identity |
ITGHK |
| 1616 |
454.7312 | 907.4478 | 907.4399 | 8.73 | 1 | 7 | 1 | 1Score > 23 indicates identity Score > 19 indicates homology |
EDFKGANK |
| 2908 |
723.8660 | 1445.7174 | 1445.7085 | 6.17 | 0 | 7 | 1 | 1Score > 22 indicates identity Score > 19 indicates homology |
SHGAVLPSYCSLR |
| 1365 |
797.3805 | 796.3732 | 796.3715 | 2.14 | 1 | 7 | 0.69 | 1Score > 20 indicates identity Score > 18 indicates homology |
RDTYDK |
| 1738 |
472.7708 | 943.5270 | 943.5226 | 4.73 | 0 | 7 | 0.66 | 1Score > 23 indicates identity Score > 18 indicates homology |
ILELNDVK + Deamidated (NQ) |
| 1016 |
586.3212 | 585.3139 | 585.3122 | 2.90 | 0 | 7 | 0.86 | 1Score > 19 indicates identity |
AGVDPK |
| 1470 |
423.2317 | 844.4488 | 844.4654 | -19.6 | 0 | 7 | 0.6 | 1Score > 24 indicates identity Score > 17 indicates homology |
NLVSGNIK + Deamidated (NQ) |
| 1263 |
368.6856 | 735.3566 | 735.3511 | 7.52 | 0 | 7 | 1 | 1Score > 21 indicates identity Score > 19 indicates homology |
VSSSNSR |
| 1242 |
363.2014 | 724.3882 | 724.3908 | -3.53 | 0 | 7 | 0.87 | 1Score > 19 indicates identity |
IGNFFK |
| 1864 |
493.2527 | 984.4908 | 984.4950 | -4.21 | 1 | 7 | 0.43 | 1Score > 20 indicates identity Score > 16 indicates homology |
YTKAMVEK + Oxidation (M) |
| 3897 |
717.6834 | 2150.0284 | 2150.0612 | -15.3 | 1 | 7 | 1 | 1Score > 22 indicates identity Score > 19 indicates homology |
RSPTAECTALGAAIAANMAFK |
| 2625 |
592.2848 | 1182.5550 | 1182.5624 | -6.24 | 1 | 7 | 0.29 | 1Score > 22 indicates identity Score > 14 indicates homology |
MTELKMNVGK + Deamidated (NQ); 2 Oxidation (M) |
| 2782 |
660.3371 | 1318.6596 | 1318.6703 | -8.10 | 1 | 7 | 0.29 | 1Score > 23 indicates identity Score > 14 indicates homology |
RSNMFVPLPNK + Deamidated (NQ); Oxidation (M) |
| 1964 |
506.2611 | 1010.5076 | 1010.5033 | 4.32 | 0 | 7 | 0.49 | 1Score > 20 indicates identity Score > 16 indicates homology |
TAGSVASGFSK |
| 3081 |
781.8981 | 1561.7816 | 1561.8100 | -18.1 | 1 | 7 | 1 | 1Score > 23 indicates identity Score > 19 indicates homology |
DINDININFAAKSK |
| 1980 |
1016.5290 | 1015.5217 | 1015.5298 | -7.94 | 0 | 7 | 1 | 1Score > 22 indicates identity Score > 19 indicates homology |
ELSLNLGNR + Deamidated (NQ) |
| 2991 |
750.8647 | 1499.7148 | 1499.7157 | -0.58 | 0 | 7 | 1 | 1Score > 22 indicates identity Score > 19 indicates homology |
YHSHNDTIFIPR + Deamidated (NQ) |
| 2050 |
518.2390 | 1034.4634 | 1034.4628 | 0.58 | 1 | 7 | 0.69 | 1Score > 20 indicates identity Score > 17 indicates homology |
ENGGSNKSDK |
| 2271 |
546.7725 | 1091.5304 | 1091.5169 | 12.4 | 0 | 7 | 0.36 | 1Score > 23 indicates identity Score > 15 indicates homology |
GLDMPVQSTK + Deamidated (NQ); Oxidation (M) |
| 2465 |
572.7885 | 1143.5624 | 1143.5673 | -4.22 | 0 | 7 | 1 | 1Score > 21 indicates identity Score > 19 indicates homology |
NSVLFGQHDK |
| 2000 |
512.2477 | 1022.4808 | 1022.4669 | 13.7 | 0 | 7 | 1 | 1Score > 21 indicates identity Score > 19 indicates homology |
LGGENNYEK |
| 1153 |
338.1770 | 674.3394 | 674.3388 | 1.02 | 0 | 7 | 0.8 | 1Score > 22 indicates identity Score > 18 indicates homology |
NWIDK |
| 2294 |
550.2746 | 1098.5346 | 1098.5557 | -19.1 | 0 | 6 | 0.25 | 1Score > 20 indicates identity Score > 13 indicates homology |
AELENLDAPK |
| 2358 |
558.3092 | 1114.6038 | 1114.6135 | -8.64 | 0 | 6 | 0.63 | 1Score > 22 indicates identity Score > 17 indicates homology |
SQILQYVHK |
| 1857 |
492.7377 | 983.4608 | 983.4784 | -17.9 | 0 | 6 | 1 | 1Score > 20 indicates identity Score > 19 indicates homology |
NPSQRPQR + 2 Deamidated (NQ) |
| 3154 |
804.3743 | 1606.7340 | 1606.7555 | -13.4 | 1 | 6 | 1 | 1Score > 21 indicates identity Score > 19 indicates homology |
NLEMSQRAEMINR + Oxidation (M) |
| 1753 |
475.7389 | 949.4632 | 949.4651 | -1.95 | 0 | 6 | 1 | 1Score > 22 indicates identity Score > 19 indicates homology |
VASSAVMNR + Oxidation (M) |
| 1819 |
486.7506 | 971.4866 | 971.4923 | -5.84 | 1 | 6 | 0.98 | 1Score > 19 indicates identity |
KQNNPELK + 2 Deamidated (NQ) |
| 1515 |
433.2074 | 864.4002 | 864.4130 | -14.7 | 1 | 6 | 1 | 1Score > 20 indicates identity Score > 19 indicates homology |
FLDREW |
| 2111 |
525.2751 | 1048.5356 | 1048.5553 | -18.7 | 0 | 6 | 1 | 1Score > 23 indicates identity Score > 19 indicates homology |
VSQFAIEVR + Deamidated (NQ) |
| 2760 |
648.8343 | 1295.6540 | 1295.6762 | -17.1 | 0 | 6 | 0.27 | 1Score > 22 indicates identity Score > 13 indicates homology |
TSFFQALGVPTK + Deamidated (NQ) |
| 1253 |
731.3581 | 730.3508 | 730.3609 | -13.8 | 0 | 6 | 0.99 | 1Score > 19 indicates identity |
ENIAER |
| 959 |
529.2992 | 528.2919 | 528.3020 | -19.0 | 0 | 6 | 0.93 | 1Score > 18 indicates identity |
AVPSR |
| 1146 |
336.2138 | 670.4130 | 670.4014 | 17.4 | 0 | 6 | 0.98 | 1Score > 19 indicates identity |
AIAPTAK |
| 1232 |
720.3817 | 719.3744 | 719.3748 | -0.55 | 1 | 6 | 1 | 1Score > 22 indicates identity Score > 19 indicates homology |
RELMR + Oxidation (M) |
| 1066 |
311.1536 | 620.2926 | 620.2918 | 1.33 | 0 | 6 | 0.58 | 1Score > 20 indicates identity Score > 16 indicates homology |
FDPSR |
| 1100 |
322.6728 | 643.3310 | 643.3289 | 3.29 | 0 | 6 | 0.71 | 1Score > 17 indicates identity |
VAPDSR |
| 2684 |
609.7891 | 1217.5636 | 1217.5676 | -3.27 | 0 | 6 | 1 | 1Score > 21 indicates identity Score > 19 indicates homology |
NAAGNFGPELAR + 2 Deamidated (NQ) |
| 2153 |
1060.5088 | 1059.5015 | 1059.4832 | 17.3 | 1 | 6 | 1 | 1Score > 22 indicates identity Score > 19 indicates homology |
NQSDPQDKK + Deamidated (NQ) |
| 3152 |
803.4147 | 1604.8148 | 1604.8409 | -16.3 | 0 | 6 | 1 | 1Score > 22 indicates identity Score > 19 indicates homology |
IVTNLNYNQALIAR + 3 Deamidated (NQ) |
| 1246 |
363.7120 | 725.4094 | 725.4224 | -17.9 | 1 | 6 | 0.62 | 1Score > 17 indicates identity |
AKWPPK |
| 2781 |
660.3300 | 1318.6454 | 1318.6703 | -18.9 | 1 | 6 | 1 | 1Score > 23 indicates identity Score > 19 indicates homology |
RSNMFVPLPNK + Deamidated (NQ); Oxidation (M) |
| 2315 |
553.2643 | 1104.5140 | 1104.4982 | 14.4 | 1 | 6 | 0.65 | 1Score > 22 indicates identity Score > 17 indicates homology |
CGRDELAER |
| 1686 |
465.7314 | 929.4482 | 929.4389 | 10.1 | 1 | 6 | 1 | 1Score > 19 indicates identity |
QHMQKNK + Deamidated (NQ); Oxidation (M) |
| 2872 |
705.8613 | 1409.7080 | 1409.7303 | -15.8 | 1 | 6 | 1 | 1Score > 21 indicates identity Score > 19 indicates homology |
FAQVIGATKGNFR + 2 Deamidated (NQ) |
| 3731 |
686.6615 | 2056.9627 | 2056.9371 | 12.4 | 1 | 6 | 1 | 1Score > 22 indicates identity Score > 19 indicates homology |
SMTPFSAGNTSSQNKLENK + Deamidated (NQ); Oxidation (M) |
| 1487 |
427.2305 | 852.4464 | 852.4527 | -7.39 | 0 | 6 | 1.2 | 1Score > 19 indicates identity |
FSIIMAR + Oxidation (M) |
| 952 |
515.3212 | 514.3139 | 514.3227 | -17.1 | 0 | 6 | 0.55 | 1Score > 22 indicates identity Score > 16 indicates homology |
AVAVR |
| 4060 |
772.0469 | 2313.1189 | 2313.0947 | 10.5 | 1 | 6 | 1 | 1Score > 23 indicates identity Score > 18 indicates homology |
LGSQVVLYGNDFDEAKAECAK |
| 1206 |
350.7166 | 699.4186 | 699.4279 | -13.2 | 0 | 6 | 1.1 | 1Score > 19 indicates identity |
NNVLLK |
| 1600 |
903.5060 | 902.4987 | 902.5073 | -9.48 | 1 | 6 | 0.36 | 1Score > 22 indicates identity Score > 14 indicates homology |
GELETKVK |
| 2203 |
537.8024 | 1073.5902 | 1073.5717 | 17.3 | 0 | 6 | 0.45 | 1Score > 22 indicates identity Score > 15 indicates homology |
TTPSVNTINK |
| 1541 |
879.4609 | 878.4536 | 878.4538 | -0.20 | 0 | 6 | 1 | 1Score > 22 indicates identity Score > 18 indicates homology |
YFPDLPK |
| 1570 |
890.4330 | 889.4257 | 889.4253 | 0.45 | 0 | 6 | 0.91 | 1Score > 22 indicates identity Score > 18 indicates homology |
ASNLSQNR + Deamidated (NQ) |
| 988 |
557.2960 | 556.2887 | 556.2969 | -14.7 | 0 | 6 | 0.62 | 1Score > 16 indicates identity |
GPNLR + Deamidated (NQ) |
| 1929 |
501.2885 | 1000.5624 | 1000.5553 | 7.16 | 0 | 6 | 0.82 | 1Score > 22 indicates identity Score > 18 indicates homology |
NLVSVINNK + Deamidated (NQ) |
| 1227 |
358.7043 | 715.3940 | 715.3864 | 10.7 | 0 | 6 | 1 | 1Score > 23 indicates identity Score > 18 indicates homology |
VEQLAR + Deamidated (NQ) |
| 1369 |
800.3804 | 799.3731 | 799.3864 | -16.6 | 0 | 6 | 0.69 | 1Score > 17 indicates identity |
YNYAIR + Deamidated (NQ) |
| 1311 |
384.2089 | 766.4032 | 766.3973 | 7.73 | 0 | 6 | 0.93 | 1Score > 18 indicates identity |
KPEQHK + Deamidated (NQ) |
| 1185 |
691.3084 | 690.3011 | 690.3007 | 0.67 | 0 | 6 | 0.67 | 1Score > 16 indicates identity |
MGNGSPK + Deamidated (NQ) |
| 2249 |
544.7735 | 1087.5324 | 1087.5145 | 16.5 | 0 | 6 | 0.89 | 1Score > 22 indicates identity Score > 18 indicates homology |
NEIIQNAQR + 3 Deamidated (NQ) |
| 1177 |
685.3902 | 684.3829 | 684.3806 | 3.37 | 0 | 6 | 0.9 | 1Score > 18 indicates identity |
AALPADK |
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