MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1801–1900 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1191 693.3712 692.3639
1192 693.4041 692.3968
1196 697.4353 696.4280
1197 697.4356 696.4283
1199 698.4125 697.4052
1200 699.3317 698.3244
1201 699.8777 698.8704
1202 700.3241 699.3168
1203 700.3271 699.3198
1204 700.3950 699.3877
1205 700.3986 699.3913
1209 701.4686 700.4613
1210 351.2390 700.4634
1211 702.3253 701.3180
1212 704.8421 703.8348
1214 707.3665 706.3592
1215 709.3521 708.3448
1216 355.2039 708.3932
1217 712.3008 711.2935
1218 712.3273 711.3200
1219 712.8377 711.8304
1220 713.3860 712.3787
1222 714.3957 713.3884
1223 357.7170 713.4194
1224 714.4288 713.4215
1225 715.3276 714.3203
1226 715.3586 714.3513
1228 717.3406 716.3333
1229 717.4284 716.4211
1230 717.4293 716.4220
1231 720.3697 719.3624
1233 721.3169 720.3096
1234 721.3869 720.3796
1235 361.6967 721.3788
1236 722.3948 721.3875
1238 362.1696 722.3246
1240 724.4061 723.3988
1241 362.7235 723.4324
1243 725.3956 724.3883
1244 726.3429 725.3356
1248 728.4453 727.4380
1249 728.8374 727.8301
1256 731.3741 730.3668
1259 367.1909 732.3672
1260 367.1913 732.3680
1261 733.3934 732.3861
1262 735.3304 734.3231
1264 736.3646 735.3573
1265 736.3653 735.3580
1266 736.3732 735.3659
1267 737.4663 736.4590
1268 737.8520 736.8447
1270 371.1805 740.3464
1271 371.1807 740.3468
1272 741.3547 740.3474
1273 741.3549 740.3476
1275 742.3836 741.3763
1276 743.3547 742.3474
1277 744.3530 743.3457
1278 746.4538 745.4465
1279 374.1714 746.3282
1280 747.3915 746.3842
1281 748.3193 747.3120
1284 752.3478 751.3405
1285 753.3617 752.3544
1287 377.7149 753.4152
1288 378.1830 754.3514
1289 755.3596 754.3523
1291 756.3533 755.3460
1292 756.3541 755.3468
1293 378.7045 755.3944
1294 757.4169 756.4096
1296 379.2175 756.4204
1297 379.6939 757.3732
1298 758.4402 757.4329
1299 758.4409 757.4336
1300 763.3653 762.3580
1301 764.4294 763.4221
1302 764.4310 763.4237
1303 764.4311 763.4238
1304 382.7312 763.4478
1305 765.3848 764.3775
1306 766.3660 765.3587
1308 767.0436 766.0363
1312 768.3528 767.3455
1313 768.3901 767.3828
1314 768.4280 767.4207
1315 768.4722 767.4649
1317 770.3696 769.3623
1318 770.4766 769.4693
1319 771.3889 770.3816
1323 772.3481 771.3408
1324 772.3498 771.3425
1326 775.8779 774.8706
1328 778.3746 777.3673
1329 778.4121 777.4048
1330 779.4165 778.4092
1331 390.2122 778.4098
1332 391.2153 780.4160
1333 781.5279 780.5206
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