MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1701–1800 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1042 304.8367 607.6588
1043 304.8368 607.6590
1044 304.8372 607.6598
1045 610.2878 609.2805
1046 610.3589 609.3516
1047 612.3001 611.2928
1048 612.3128 611.3055
1049 612.3376 611.3303
1050 612.3475 611.3402
1051 306.6775 611.3404
1052 306.6777 611.3408
1053 613.3622 612.3549
1054 613.3632 612.3559
1055 614.2784 613.2711
1056 614.2803 613.2730
1057 614.3164 613.3091
1062 618.3222 617.3149
1068 624.3367 623.3294
1069 624.3376 623.3303
1071 626.3162 625.3089
1072 626.3171 625.3098
1073 627.3125 626.3052
1074 628.2956 627.2883
1075 314.6750 627.3354
1078 315.6647 629.3148
1081 634.2820 633.2747
1083 635.3079 634.3006
1086 638.3218 637.3145
1087 320.1772 638.3398
1088 320.1776 638.3406
1090 321.1849 640.3552
1093 642.3148 641.3075
1094 643.3552 642.3479
1095 644.2648 643.2575
1105 645.4068 644.3995
1106 645.4070 644.3997
1107 648.3735 647.3662
1108 649.3660 648.3587
1109 325.3501 648.6856
1110 325.3501 648.6856
1111 325.3502 648.6858
1112 325.3503 648.6860
1113 650.3859 649.3786
1114 650.3916 649.3843
1115 651.3418 650.3345
1116 326.1829 650.3512
1117 652.3325 651.3252
1118 653.3017 652.2944
1119 327.1909 652.3672
1120 654.3593 653.3520
1121 654.3600 653.3527
1125 329.1826 656.3506
1126 657.3861 656.3788
1128 661.3902 660.3829
1129 664.9785 663.9712
1130 665.3646 664.3573
1131 665.3749 664.3676
1132 333.1913 664.3680
1133 667.3070 666.2997
1134 667.3315 666.3242
1135 667.3547 666.3474
1136 334.1819 666.3492
1137 668.3108 667.3035
1138 669.3220 668.3147
1139 669.3233 668.3160
1141 669.3286 668.3213
1143 669.3584 668.3511
1144 670.3166 669.3093
1147 671.4226 670.4153
1148 671.4230 670.4157
1150 673.3178 672.3105
1151 337.1798 672.3450
1152 337.6620 673.3094
1154 339.6646 677.3146
1156 678.3599 677.3526
1157 679.3790 678.3717
1158 680.3666 679.3593
1159 680.4426 679.4353
1160 680.4431 679.4358
1162 681.3582 680.3509
1163 681.3693 680.3620
1164 341.1889 680.3632
1165 341.2008 680.3870
1167 681.8151 680.8078
1168 682.3306 681.3233
1170 683.3765 682.3692
1171 342.1960 682.3774
1172 684.4001 683.3928
1173 684.4174 683.4101
1174 685.3445 684.3372
1175 685.3771 684.3698
1176 343.1980 684.3814
1178 686.3736 685.3663
1180 687.4028 686.3955
1181 687.4038 686.3965
1186 691.4245 690.4172
1187 691.8569 690.8496
1188 692.4448 691.4375
1189 692.8275 691.8202
1190 692.8351 691.8278
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