MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1501–1600 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
815 439.2164 438.2091
816 439.2165 438.2092
817 439.2166 438.2093
818 439.2168 438.2095
819 439.2170 438.2097
820 439.2172 438.2099
821 439.2173 438.2100
822 439.2176 438.2103
823 439.2177 438.2104
824 439.2184 438.2111
825 439.2186 438.2113
826 439.2187 438.2114
827 439.2950 438.2877
828 441.2100 440.2027
829 441.2112 440.2039
830 441.2301 440.2228
831 441.2321 440.2248
832 441.2347 440.2274
833 441.2350 440.2277
834 442.2424 441.2351
835 442.2427 441.2354
836 442.2673 441.2600
837 442.7338 441.7265
838 443.2457 442.2384
839 443.2470 442.2397
840 443.2476 442.2403
841 443.2478 442.2405
842 443.2482 442.2409
843 443.2484 442.2411
844 443.2487 442.2414
845 443.2489 442.2416
846 443.2494 442.2421
847 443.2879 442.2806
848 443.3284 442.3211
849 444.2813 443.2740
850 446.2617 445.2544
851 447.2776 446.2703
852 447.2832 446.2759
853 449.1516 448.1443
854 451.2509 450.2436
855 455.2124 454.2051
856 455.2134 454.2061
857 455.3287 454.3214
858 455.3296 454.3223
859 456.2495 455.2422
860 457.2258 456.2185
861 457.2270 456.2197
862 457.2274 456.2201
863 457.2274 456.2201
864 457.2277 456.2204
865 457.2278 456.2205
866 457.2279 456.2206
867 457.2282 456.2209
868 457.2289 456.2216
869 457.2291 456.2218
870 457.2295 456.2222
871 457.3453 456.3380
872 458.2112 457.2039
873 458.2376 457.2303
874 458.2621 457.2548
875 458.2993 457.2920
876 459.1034 458.0961
877 459.2202 458.2129
878 459.2417 458.2344
879 459.2833 458.2760
880 459.2848 458.2775
881 461.2570 460.2497
882 461.2584 460.2511
883 461.2588 460.2515
884 463.2587 462.2514
885 464.7417 463.7344
886 466.2432 465.2359
887 467.2148 466.2075
888 467.2518 466.2445
889 469.2426 468.2353
890 472.2412 471.2339
891 472.2417 471.2344
893 472.2787 471.2714
894 472.2909 471.2836
895 473.3005 472.2932
896 473.3032 472.2959
897 473.3038 472.2965
898 474.2299 473.2226
899 474.2315 473.2242
900 474.2566 473.2493
901 474.7939 473.7866
902 475.2981 474.2908
903 477.1136 476.1063
904 484.3145 483.3072
905 486.2579 485.2506
906 486.2753 485.2680
908 487.3179 486.3106
909 488.1945 487.1872
910 488.2361 487.2288
911 488.2362 487.2289
912 488.3107 487.3034
913 493.0201 492.0128
914 493.0218 492.0145
915 493.0219 492.0146
916 493.0219 492.0146
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