MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1401–1500 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
715 409.2438 408.2365
716 410.2439 409.2366
717 410.2450 409.2377
718 410.2450 409.2377
719 410.2452 409.2379
720 411.2202 410.2129
721 412.2607 411.2534
722 413.2708 412.2635
723 413.2710 412.2637
724 413.2712 412.2639
725 413.2716 412.2643
726 413.2717 412.2644
727 413.2718 412.2645
728 413.2734 412.2661
729 414.1993 413.1920
730 414.2354 413.2281
731 414.2356 413.2283
732 414.2361 413.2288
733 414.2747 413.2674
734 415.2139 414.2066
735 415.2387 414.2314
736 415.2943 414.2870
737 415.2948 414.2875
738 417.2357 416.2284
739 417.2549 416.2476
740 418.2744 417.2671
741 418.2748 417.2675
742 418.2749 417.2676
743 418.2756 417.2683
744 421.2047 420.1974
745 421.2059 420.1986
746 421.2060 420.1987
747 421.2060 420.1987
748 421.2061 420.1988
749 421.2064 420.1991
750 421.2064 420.1991
751 421.2068 420.1995
752 421.2071 420.1998
753 421.2074 420.2001
754 421.2076 420.2003
755 421.2077 420.2004
756 421.2077 420.2004
757 423.1996 422.1923
758 423.2006 422.1933
759 423.2007 422.1934
760 423.2013 422.1940
761 423.2202 422.2129
762 423.2215 422.2142
763 423.2216 422.2143
764 423.2216 422.2143
765 423.2219 422.2146
766 423.2220 422.2147
767 423.2223 422.2150
768 423.2230 422.2157
769 423.2230 422.2157
770 423.2230 422.2157
771 423.2231 422.2158
772 423.2234 422.2161
773 423.2236 422.2163
774 423.2237 422.2164
775 423.2917 422.2844
776 425.2034 424.1961
777 425.2357 424.2284
778 425.2370 424.2297
779 425.2371 424.2298
780 425.2373 424.2300
781 425.2375 424.2302
782 425.2375 424.2302
783 425.2379 424.2306
784 425.2780 424.2707
785 425.2912 424.2839
786 425.2912 424.2839
787 425.2920 424.2847
788 425.2921 424.2848
789 425.2926 424.2853
790 426.2413 425.2340
791 426.2413 425.2340
792 427.2536 426.2463
793 427.2536 426.2463
794 427.2939 426.2866
795 428.2549 427.2476
796 428.2556 427.2483
797 428.2557 427.2484
798 428.2558 427.2485
799 428.2560 427.2487
800 428.2566 427.2493
801 430.2303 429.2230
802 430.2303 429.2230
803 430.2303 429.2230
804 430.3012 429.2939
805 430.3021 429.2948
806 430.3024 429.2951
807 430.3029 429.2956
808 431.2191 430.2118
809 431.2880 430.2807
810 435.2640 434.2567
811 437.1967 436.1894
812 437.2018 436.1945
813 438.1991 437.1918
814 439.2148 438.2075
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