MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1301–1400 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
615 383.2280 382.2207
616 383.2281 382.2208
617 383.2283 382.2210
618 383.2287 382.2214
619 383.2288 382.2215
620 383.2297 382.2224
621 383.2298 382.2225
622 383.2303 382.2230
623 385.2026 384.1953
624 385.2044 384.1971
625 385.2047 384.1974
626 385.2437 384.2364
627 385.2452 384.2379
628 385.2456 384.2383
629 387.2383 386.2310
630 387.2589 386.2516
631 387.2620 386.2547
632 389.2142 388.2069
633 389.2400 388.2327
634 389.2414 388.2341
635 391.2307 390.2234
636 391.2866 390.2793
637 391.2877 390.2804
638 391.2880 390.2807
639 391.2881 390.2808
640 391.2881 390.2808
641 391.2883 390.2810
642 391.2887 390.2814
643 391.2887 390.2814
644 391.2893 390.2820
645 392.2579 391.2506
646 392.2594 391.2521
647 393.2455 392.2382
648 393.2472 392.2399
649 393.2472 392.2399
650 393.2474 392.2401
651 393.2479 392.2406
652 393.2997 392.2924
653 393.3011 392.2938
654 393.3013 392.2940
655 393.3014 392.2941
656 393.3015 392.2942
657 393.3015 392.2942
658 393.3016 392.2943
659 393.3020 392.2947
660 393.3023 392.2950
661 393.3025 392.2952
662 393.3025 392.2952
663 393.3025 392.2952
664 393.3026 392.2953
665 393.3029 392.2956
666 393.3029 392.2956
667 393.3029 392.2956
668 395.2249 394.2176
669 395.2264 394.2191
670 395.2264 394.2191
671 395.2612 394.2539
672 395.2623 394.2550
673 395.2623 394.2550
674 395.2627 394.2554
675 397.2053 396.1980
676 397.2058 396.1985
677 397.2063 396.1990
678 397.2065 396.1992
679 397.2068 396.1995
680 397.2069 396.1996
681 397.2072 396.1999
682 398.2791 397.2718
683 399.2204 398.2131
684 399.2225 398.2152
685 399.2232 398.2159
686 399.2233 398.2160
687 399.2236 398.2163
688 399.2257 398.2184
689 399.2631 398.2558
690 401.2072 400.1999
691 401.2365 400.2292
692 401.2781 400.2708
693 401.3131 400.3058
694 402.2467 401.2394
695 405.2103 404.2030
696 405.2109 404.2036
697 405.2110 404.2037
698 405.2112 404.2039
699 407.2249 406.2176
700 407.2263 406.2190
701 407.2264 406.2191
702 407.2265 406.2192
703 407.2267 406.2194
704 407.2267 406.2194
705 407.2270 406.2197
706 407.2273 406.2200
707 407.2274 406.2201
708 407.2274 406.2201
709 409.2422 408.2349
710 409.2422 408.2349
711 409.2423 408.2350
712 409.2426 408.2353
713 409.2428 408.2355
714 409.2433 408.2360
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