MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1201–1300 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
515 369.2282 368.2209
516 369.2282 368.2209
517 369.2283 368.2210
518 369.2284 368.2211
519 369.2285 368.2212
520 369.2287 368.2214
521 369.2288 368.2215
522 369.2291 368.2218
523 369.2293 368.2220
524 369.2489 368.2416
525 369.2489 368.2416
526 369.2490 368.2417
527 369.2492 368.2419
528 369.2492 368.2419
529 369.2493 368.2420
530 370.2318 369.2245
531 370.2509 369.2436
532 370.2521 369.2448
533 371.2293 370.2220
534 371.2294 370.2221
535 371.3170 370.3097
536 371.3172 370.3099
537 371.3172 370.3099
538 371.3178 370.3105
539 371.3181 370.3108
540 371.3182 370.3109
541 371.3185 370.3112
542 371.3191 370.3118
543 371.3191 370.3118
544 371.3192 370.3119
545 371.3193 370.3120
546 371.3194 370.3121
547 371.3195 370.3122
548 371.3196 370.3123
549 371.3199 370.3126
550 371.3201 370.3128
551 371.3201 370.3128
552 371.3202 370.3129
553 371.3202 370.3129
554 371.3203 370.3130
555 371.3203 370.3130
556 371.3205 370.3132
557 371.3205 370.3132
558 371.3205 370.3132
559 371.3206 370.3133
560 371.3206 370.3133
561 373.2084 372.2011
562 373.2086 372.2013
563 373.2459 372.2386
564 373.2823 372.2750
565 374.1671 373.1598
566 374.2044 373.1971
567 375.2241 374.2168
568 376.2256 375.2183
569 378.2425 377.2352
570 379.1868 378.1795
571 379.1951 378.1878
572 379.1952 378.1879
573 379.1952 378.1879
574 379.1952 378.1879
575 379.1955 378.1882
576 379.1956 378.1883
577 379.1958 378.1885
578 379.1959 378.1886
579 379.2298 378.2225
580 381.1921 380.1848
581 381.1923 380.1850
582 381.1927 380.1854
583 381.1928 380.1855
584 381.1928 380.1855
585 381.1935 380.1862
586 381.2094 380.2021
587 381.2110 380.2037
588 381.2112 380.2039
589 381.2113 380.2040
590 381.2115 380.2042
591 381.2119 380.2046
592 381.2120 380.2047
593 381.2122 380.2049
594 381.2128 380.2055
595 381.2132 380.2059
596 381.2147 380.2074
597 381.2456 380.2383
598 383.1899 382.1826
599 383.1906 382.1833
600 383.2072 382.1999
601 383.2078 382.2005
602 383.2078 382.2005
603 383.2078 382.2005
604 383.2080 382.2007
605 383.2081 382.2008
606 383.2082 382.2009
607 383.2086 382.2013
608 383.2089 382.2016
609 383.2090 382.2017
610 383.2110 382.2037
611 383.2262 382.2189
612 383.2277 382.2204
613 383.2278 382.2205
614 383.2278 382.2205
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