MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 901–1000 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
215 325.1832 324.1759
216 325.1832 324.1759
217 325.1835 324.1762
218 325.1837 324.1764
219 325.1841 324.1768
220 325.1849 324.1776
221 325.1850 324.1777
222 325.1852 324.1779
223 325.1856 324.1783
224 325.2013 324.1940
225 325.2018 324.1945
226 325.2025 324.1952
227 325.2032 324.1959
228 325.2218 324.2145
229 325.2220 324.2147
230 325.2221 324.2148
231 326.1478 325.1405
232 327.1332 326.1259
233 327.1987 326.1914
234 327.2013 326.1940
235 327.2015 326.1942
236 327.2015 326.1942
237 327.2017 326.1944
238 327.2019 326.1946
239 327.2177 326.2104
240 328.2241 327.2168
241 329.1782 328.1709
242 329.2163 328.2090
243 331.1893 330.1820
244 331.1904 330.1831
245 331.1905 330.1832
246 331.1905 330.1832
247 331.1907 330.1834
248 331.1907 330.1834
249 331.1908 330.1835
250 331.1908 330.1835
251 331.1910 330.1837
252 331.2112 330.2039
253 331.2113 330.2040
254 331.2115 330.2042
255 333.0687 332.0614
256 333.2056 332.1983
257 333.2065 332.1992
258 333.2065 332.1992
259 333.2066 332.1993
260 333.2066 332.1993
261 333.2070 332.1997
262 333.2070 332.1997
263 333.2072 332.1999
264 333.2072 332.1999
265 333.2073 332.2000
266 333.2076 332.2003
267 333.2077 332.2004
268 333.2242 332.2169
269 333.2272 332.2199
270 333.2273 332.2200
271 333.2273 332.2200
272 333.2274 332.2201
273 333.2274 332.2201
274 335.1859 334.1786
275 335.1863 334.1790
276 335.1871 334.1798
277 335.1874 334.1801
278 335.2216 334.2143
279 335.2222 334.2149
280 335.2223 334.2150
281 335.2224 334.2151
282 335.2224 334.2151
283 335.2225 334.2152
284 335.2226 334.2153
285 335.2229 334.2156
286 335.2231 334.2158
287 335.2232 334.2159
288 335.2234 334.2161
289 335.2236 334.2163
290 335.2406 334.2333
291 335.2426 334.2353
292 335.2428 334.2355
293 336.2258 335.2185
294 337.1829 336.1756
295 337.1836 336.1763
296 337.1836 336.1763
297 337.1837 336.1764
298 337.1838 336.1765
299 337.1839 336.1766
300 337.1839 336.1766
301 337.1844 336.1771
302 337.1848 336.1775
303 337.1851 336.1778
304 337.1859 336.1786
305 337.2007 336.1934
306 337.2013 336.1940
307 337.2014 336.1941
308 337.2016 336.1943
309 337.2016 336.1943
310 337.2019 336.1946
311 337.2020 336.1947
312 337.2022 336.1949
313 337.2574 336.2501
314 337.2578 336.2505
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