MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1–100 (out of 4700)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1080 316.6852 631.3558 631.3541 2.80 0 22 0.15 +1Score > 27 indicates identity VLSEGK
2707 617.3135 1232.6124 1232.6070 4.39 1 21 0.086 +1Score > 23 indicates identity MNKIQQINDK + 2 Deamidated (NQ)
1064 618.3315 617.3242 617.3207 5.74 0 19 0.08 +1Score > 23 indicates identity
Score > 21 indicates homology		 MGGLPK + Oxidation (M)
1183 344.7165 687.4184 687.4167 2.56 0 19 0.095 +1Score > 22 indicates identity TEIVVK
1367 399.7076 797.4006 797.3959 5.89 0 18 0.078 +1Score > 19 indicates identity FDEFIK
1351 393.7414 785.4682 785.4647 4.55 0 17 0.13 +1Score > 21 indicates identity LNGLELK
1070 313.1877 624.3608 624.3595 2.16 0 17 0.07 +1Score > 18 indicates identity HQLVK + Deamidated (NQ)
1423 414.2219 826.4292 826.4409 -14.1 1 17 0.1 +1Score > 19 indicates identity NEGPVRR
1255 366.1890 730.3634 730.3722 -12.0 1 16 0.13 +1Score > 20 indicates identity DGINRR + Deamidated (NQ)
2303 1103.5101 1102.5028 1102.5142 -10.3 0 16 0.11 +1Score > 19 indicates identity EGGETQEKPK + Deamidated (NQ)
1469 423.2231 844.4316 844.4403 -10.2 1 15 0.2 +1Score > 22 indicates identity
Score > 21 indicates homology		 EGGREGLK
1149 673.3173 672.3100 672.3231 -19.5 0 15 0.071 +1Score > 16 indicates identity DWAPGK
1565 443.7686 885.5226 885.5171 6.26 0 15 0.25 +1Score > 21 indicates identity VGEIINLK + Deamidated (NQ)
1207 701.3593 700.3520 700.3504 2.36 0 15 0.13 +1Score > 18 indicates identity INPSNR + Deamidated (NQ)
1453 421.2448 840.4750 840.4705 5.42 0 15 0.11 +1Score > 17 indicates identity INIPAGQK + Deamidated (NQ)
1747 474.2411 946.4676 946.4832 -16.4 0 15 0.078 +1Score > 23 indicates identity
Score > 16 indicates homology		 AGSIQTQSR
1468 845.4375 844.4302 844.4403 -11.9 1 14 0.3 +1Score > 22 indicates identity KQQGGGIR + 2 Deamidated (NQ)
2372 1122.5090 1121.5017 1121.5209 -17.1 0 14 0.1 +1Score > 21 indicates identity
Score > 17 indicates homology		 AGGEGMCIAIK + Oxidation (M)
3713 1023.0363 2044.0580 2044.0768 -9.17 1 14 0.07 +1Score > 21 indicates identity
Score > 15 indicates homology		 IENFPVKIEQIIPPNYK + 3 Deamidated (NQ)
1058 615.3113 614.3040 614.3024 2.70 0 13 0.11 +1Score > 16 indicates identity QGNAPK + Deamidated (NQ)
1858 984.4685 983.4612 983.4784 -17.5 0 13 0.25 +1Score > 20 indicates identity NPSQRPQR + 2 Deamidated (NQ)
2739 636.3207 1270.6268 1270.6404 -10.7 1 13 0.26 +1Score > 22 indicates identity
Score > 20 indicates homology		 KNPEEQIAVNK + 2 Deamidated (NQ)
1494 428.2513 854.4880 854.4974 -10.9 1 13 0.27 +1Score > 20 indicates identity QPINKQK
2757 646.3370 1290.6594 1290.6489 8.16 1 13 0.5 +1Score > 23 indicates identity NAVGILGNMEKK + 2 Deamidated (NQ); Oxidation (M)
2808 671.8506 1341.6866 1341.6928 -4.59 1 13 0.24 +1Score > 22 indicates identity
Score > 19 indicates homology		 EAPNYHQIIKK + 2 Deamidated (NQ)
1464 843.4942 842.4869 842.4861 0.92 0 13 0.38 +1Score > 21 indicates identity NIDIVLR + Deamidated (NQ)
1063 309.6666 617.3186 617.3207 -3.29 0 13 0.47 +1Score > 23 indicates identity
Score > 22 indicates homology		 MGGLPK + Oxidation (M)
1375 400.7361 799.4576 799.4552 3.08 1 13 0.23 +1Score > 20 indicates identity
Score > 19 indicates homology		 ATDPKLR
2492 575.2873 1148.5600 1148.5713 -9.80 0 12 0.26 +1Score > 22 indicates identity
Score > 19 indicates homology		 NLPSLEANYK + Deamidated (NQ)
2035 516.2645 1030.5144 1030.5182 -3.66 0 12 0.24 +1Score > 24 indicates identity
Score > 19 indicates homology		 LQGNLDELK + 2 Deamidated (NQ)
1065 619.3495 618.3422 618.3337 13.8 0 12 0.26 +1Score > 23 indicates identity
Score > 19 indicates homology		 SNGTLK
3686 1012.0351 2022.0556 2022.0568 -0.57 1 12 0.39 +1Score > 21 indicates identity NVVNLLNTQMKNISGSFK + Oxidation (M)
2562 583.2982 1164.5818 1164.5775 3.75 0 12 0.62 +1Score > 23 indicates identity IPGLNPGDEPR + Deamidated (NQ)
1101 644.3385 643.3312 643.3289 3.56 0 12 0.18 +1Score > 17 indicates identity LGSPDR
1874 494.7823 987.5500 987.5601 -10.1 1 12 0.52 +1Score > 22 indicates identity
Score > 22 indicates homology		 DGVLDIKTK
1796 482.2451 962.4756 962.4743 1.43 0 12 0.6 +1Score > 23 indicates identity
Score > 22 indicates homology		 VLDCTNLK + Deamidated (NQ)
1917 500.2591 998.5036 998.5145 -10.8 1 12 0.36 +1Score > 20 indicates identity EKSLHTER
2079 1042.5183 1041.5110 1041.5277 -16.0 0 12 0.38 +1Score > 20 indicates identity
Score > 20 indicates homology		 MEIHSNLAK
2693 611.8120 1221.6094 1221.5877 17.8 0 12 0.16 +1Score > 23 indicates identity
Score > 16 indicates homology		 AELFITNQER + 2 Deamidated (NQ)
2105 1048.5547 1047.5474 1047.5461 1.22 1 12 0.096 +1Score > 23 indicates identity
Score > 14 indicates homology		 ARGVEVDFR
1772 477.7503 953.4860 953.4679 19.1 1 11 0.27 +1Score > 20 indicates identity
Score > 18 indicates homology		 EPNHSRSK
1099 322.6723 643.3300 643.3289 1.73 0 11 0.23 +1Score > 18 indicates identity LGSPDR
2925 724.3885 1446.7624 1446.7388 16.3 0 11 0.63 +1Score > 22 indicates identity NQVLIIMGETGSGK + Deamidated (NQ)
2718 626.8150 1251.6154 1251.6064 7.27 1 11 0.98 +1Score > 24 indicates identity MSLKQMQSQR + Oxidation (M)
2151 1059.5092 1058.5019 1058.5066 -4.42 0 11 0.48 +1Score > 21 indicates identity LSNEMHALK + Deamidated (NQ); Oxidation (M)
3184 544.6077 1630.8013 1630.7910 6.28 1 11 0.84 +1Score > 23 indicates identity NSTNASVAGNISNPKR + 2 Deamidated (NQ)
2109 525.2697 1048.5248 1048.5223 2.47 1 11 0.26 +1Score > 22 indicates identity
Score > 18 indicates homology		 MLRQIQDK + 2 Deamidated (NQ); Oxidation (M)
2924 724.3857 1446.7568 1446.7579 -0.74 0 11 0.71 +1Score > 22 indicates identity LGATIDVEHSHLR
1030 601.3644 600.3571 600.3595 -3.93 0 11 0.52 +1Score > 21 indicates identity NILNK
1307 766.3826 765.3753 765.3769 -2.09 1 11 0.34 +1Score > 21 indicates identity
Score > 19 indicates homology		 FNKSNR + Deamidated (NQ)
2923 724.3848 1446.7550 1446.7579 -1.98 0 11 0.74 +1Score > 22 indicates identity LGATIDVEHSHLR
1098 644.3371 643.3298 643.3289 1.38 0 11 0.27 +1Score > 18 indicates identity LGSPDR
2694 611.8126 1221.6106 1221.5877 18.8 0 11 0.87 +1Score > 23 indicates identity AELFITNQER + 2 Deamidated (NQ)
1079 630.3282 629.3209 629.3133 12.1 0 11 0.17 +1Score > 16 indicates identity TAQPGR + Deamidated (NQ)
1744 947.4514 946.4441 946.4430 1.23 0 11 0.77 +1Score > 22 indicates identity STPANTPMK + Deamidated (NQ)
2626 1183.5630 1182.5557 1182.5703 -12.3 1 10 0.18 +1Score > 22 indicates identity
Score > 16 indicates homology		 LGSDKFTMQR + Deamidated (NQ)
1169 342.1806 682.3466 682.3439 4.09 0 10 0.3 +1Score > 18 indicates identity FNFQK
1376 801.4812 800.4739 800.4644 12.0 0 10 0.73 +1Score > 22 indicates identity VLIDNVK + Deamidated (NQ)
2871 705.8550 1409.6954 1409.7006 -3.68 1 10 0.4 +1Score > 21 indicates identity
Score > 19 indicates homology		 NTNISIMRLGMK + Deamidated (NQ); 2 Oxidation (M)
1611 906.4334 905.4261 905.4277 -1.69 0 10 0.21 +1Score > 23 indicates identity
Score > 16 indicates homology		 AVPSSSACK
3079 778.8474 1555.6802 1555.6638 10.6 0 10 0.61 +1Score > 21 indicates identity QIQQQQQQQQHK + 7 Deamidated (NQ)
1457 842.4390 841.4317 841.4406 -10.5 1 10 0.31 +1Score > 18 indicates identity QNPAERK
1726 471.2314 940.4482 940.4654 -18.2 0 10 0.51 +1Score > 20 indicates identity FLLDNYR + Deamidated (NQ)
966 531.2781 530.2708 530.2813 -19.7 0 10 0.53 +1Score > 23 indicates identity
Score > 20 indicates homology		 GIGER
2469 573.2867 1144.5588 1144.5724 -11.8 0 10 0.24 +1Score > 22 indicates identity
Score > 16 indicates homology		 ENGLLNEISR + Deamidated (NQ)
3065 773.9080 1545.8014 1545.8012 0.18 1 10 0.92 +1Score > 22 indicates identity VASHKNSHNPSLVR + Deamidated (NQ)
1374 400.7195 799.4244 799.4301 -7.03 0 10 0.33 +1Score > 18 indicates identity LLGGGGGGGR
936 501.2677 500.2604 500.2594 1.96 0 10 0.1 +1Score > 13 indicates identity EAGPK
3419 911.8907 1821.7668 1821.8030 -19.8 1 10 0.29 +1Score > 17 indicates identity AVQHKVYNNHNSHDR + 4 Deamidated (NQ)
2920 724.3574 1446.7002 1446.6959 2.98 0 10 0.99 +1Score > 22 indicates identity LNGGLGTSMGCVGPK
3054 769.8602 1537.7058 1537.7341 -18.4 1 10 0.22 +1Score > 21 indicates identity
Score > 16 indicates homology		 TNSRMLLMNGNVR + Deamidated (NQ); 2 Oxidation (M)
2539 1160.5620 1159.5547 1159.5770 -19.2 0 9 0.8 +1Score > 21 indicates identity MVPTMFPPPK + Oxidation (M)
2141 528.7718 1055.5290 1055.5360 -6.54 1 9 0.57 +1Score > 20 indicates identity
Score > 19 indicates homology		 KESQPPNTR
1102 644.3387 643.3314 643.3289 3.87 0 9 0.37 +1Score > 17 indicates identity LGSPDR
1208 351.1836 700.3526 700.3392 19.2 0 9 0.44 +1Score > 18 indicates identity LDDGGPK
2747 639.3314 1276.6482 1276.6636 -12.0 1 9 1 +1Score > 23 indicates identity
Score > 22 indicates homology		 SPTNNNPRIHK
1310 767.4069 766.3996 766.3973 3.00 0 9 0.42 +1Score > 20 indicates identity
Score > 18 indicates homology		 KPEQHK + Deamidated (NQ)
2725 630.3047 1258.5948 1258.6194 -19.5 0 9 0.49 +1Score > 21 indicates identity
Score > 18 indicates homology		 DADLGWGGLINK + Deamidated (NQ)
1096 644.3268 643.3195 643.3289 -14.6 0 9 0.29 +1Score > 16 indicates identity SGVEPR
1254 731.3600 730.3527 730.3497 4.14 1 9 0.52 +1Score > 19 indicates identity KNDEPK + Deamidated (NQ)
2711 620.7944 1239.5742 1239.5983 -19.4 0 9 0.31 +1Score > 20 indicates identity
Score > 16 indicates homology		 TESNEEIPPPK
1269 370.7012 739.3878 739.3864 1.90 0 9 0.5 +1Score > 18 indicates identity VDHELK
1959 505.2711 1008.5276 1008.5314 -3.71 1 9 0.93 +1Score > 21 indicates identity MVDKIFEK
1213 705.3203 704.3130 704.3089 5.81 0 9 0.47 -1Score > 18 indicates identity GNSGVDR + Deamidated (NQ)
3600 992.0056 1981.9966 1981.9817 7.54 0 9 1 +1Score > 22 indicates identity
Score > 21 indicates homology		 TALQQQIHAAQQATNTTR + 2 Deamidated (NQ)
1059 616.2944 615.2871 615.2864 1.21 0 8 0.25 +1Score > 15 indicates identity QGNAPK + 2 Deamidated (NQ)
2713 414.5518 1240.6336 1240.6339 -0.30 0 8 0.19 +1Score > 23 indicates identity
Score > 14 indicates homology		 YVLYGVSTPNK + Deamidated (NQ)
3086 781.9057 1561.7968 1561.8239 -17.3 0 8 0.17 +1Score > 23 indicates identity
Score > 13 indicates homology		 ATLSTLFQELNVPK + 2 Deamidated (NQ)
2147 529.7938 1057.5730 1057.5767 -3.47 1 8 0.25 +1Score > 22 indicates identity
Score > 15 indicates homology		 KAELIENNK
2850 695.8749 1389.7352 1389.7252 7.24 1 8 0.51 +1Score > 22 indicates identity
Score > 18 indicates homology		 IRELLNFNDEK
2952 738.3674 1474.7202 1474.7456 -17.2 1 8 1 +1Score > 22 indicates identity
Score > 21 indicates homology		 GIQEIKDHPYFK + Deamidated (NQ)
1124 329.1825 656.3504 656.3605 -15.4 0 8 0.36 +1Score > 16 indicates identity LNAPSR
1320 386.2039 770.3932 770.4035 -13.3 1 8 0.52 +1Score > 18 indicates identity QGPQGKR + Deamidated (NQ)
1824 487.2614 972.5082 972.5240 -16.2 1 8 0.85 +1Score > 23 indicates identity
Score > 20 indicates homology		 KNIDENIK
933 499.2883 498.2810 498.2802 1.69 0 8 0.16 +1Score > 13 indicates identity APPSK
2062 519.7476 1037.4806 1037.4778 2.78 0 8 0.19 +1Score > 21 indicates identity
Score > 13 indicates homology		 IGETDYNAR
1537 878.4486 877.4413 877.4327 9.80 1 8 0.51 +1Score > 24 indicates identity
Score > 18 indicates homology		 KSLEMDR
1794 963.4709 962.4636 962.4556 8.29 1 8 0.3 +1Score > 23 indicates identity
Score > 15 indicates homology		 VTDEDKEK
1573 890.4432 889.4359 889.4327 3.60 0 8 0.43 +1Score > 23 indicates identity
Score > 17 indicates homology		 QMENVLR + Deamidated (NQ)
1809 967.4905 966.4832 966.5022 -19.6 0 8 0.18 +1Score > 22 indicates identity
Score > 13 indicates homology		 QLDTYISK
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