MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 1 |
| MS data file | : | PRT1245_Other_P1_B9.mgf |
| Database | : | S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues) |
| Timestamp | : | 24 Jun 2025 at 21:21:15 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,728 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q9FD70_ENTFL | 101 | Acetyl-CoA acetyltransferase/HMG-CoA reductase OS=Enterococcus faecalis GN=mvaE PE=3 SV=1 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PGK_YEAST | 98 | Phosphoglycerate kinase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PGK1 PE=1 SV=2 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ENO1_YEAST | 45 | Enolase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ENO1 PE=1 SV=3 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PUS9_YEAST | 36 | tRNA pseudouridine(32) synthase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PUS9 PE=1 SV=1 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | BRR6_YEAST | 30 | Nucleus export protein BRR6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=BRR6 PE=1 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | COG8_YEAST | 27 | Conserved oligomeric Golgi complex subunit 8 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=COG8 PE=1 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | APE2_YEAST | 24 | Aminopeptidase 2, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=APE2 PE=1 SV=4 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPF1_YEAST | 23 | Ribosome production factor 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RPF1 PE=1 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 8.1 | RPF1_YEAST |
23 | 35157 | 2 (1) | 2 (1) | 0.09 | |
| Ribosome production factor 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RPF1 PE=1 SV=1 | |||||||
2 peptide matches (2 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1257 |
366.2094 | 730.4042 | 730.3974 | 9.44 | 0 | 23 | 0.051 | 1Score > 23 indicates identity |
U | R.LGQTVGR.L + Deamidated (NQ) | |
D:\Xcalibur\Data\Yan\PRT1245 redo\PRT1245_Other_P1_B9.rawScore > 22 indicates identity Score > 14 indicates homology |
|||||||||||
| 9.68 | 0 | 4 | 0.52 | 1 | VENGIMQSISEK | ||||||
| 3.78 | 0 | 1 | 1.1 | 2 | DNINDQIFSLR | ||||||
| 2800 |
667.8411 | 1333.6676 | 1333.6547 | 9.69 | 0 | 0 | 1.2 | 3Score > 22 indicates identity Score > 14 indicates homology |
U | -.MALGNEINITNK.L + Deamidated (NQ); Oxidation (M) | |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | B211p2_002|NRPSb | 22 | OS=Anaeromyces robustus OX=1754192 PE=2 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AVO2_YEAST | 22 | Target of rapamycin complex 2 subunit AVO2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=AVO2 PE=1 SV=1 |
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