MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Quantitation overview (16 proteins)

Score Mass Matches Sequences emPAI
Score Mass Matches Sequences emPAI
1 Q9FD70_ENTFL 101 86614 2 (2) 2 (2) 0.08 Acetyl-CoA acetyltransferase/HMG-CoA reductase OS=Enterococcus faecalis GN=mvaE PE=3 SV=1
2 PGK_YEAST 98 44768 2 (2) 1 (1) 0.07 Phosphoglycerate kinase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PGK1 PE=1 SV=2
3 ENO1_YEAST 45 46844 5 (3) 3 (1) 0.15 Enolase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ENO1 PE=1 SV=3
4 PUS9_YEAST 36 53765 2 (1) 2 (1) 0.06 tRNA pseudouridine(32) synthase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PUS9 PE=1 SV=1
5 BRR6_YEAST 30 23021 1 (1) 1 (1) 0.15 Nucleus export protein BRR6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=BRR6 PE=1 SV=1
6 COG8_YEAST 27 70128 1 (1) 1 (1) 0.05 Conserved oligomeric Golgi complex subunit 8 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=COG8 PE=1 SV=1
7 APE2_YEAST 24 108086 1 (1) 1 (1) 0.03 Aminopeptidase 2, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=APE2 PE=1 SV=4
8 RPF1_YEAST 23 35157 2 (1) 2 (1) 0.09 Ribosome production factor 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RPF1 PE=1 SV=1
9 B211p2_002|NRPSb 22 633771 2 (1) 2 (1) 0.01 OS=Anaeromyces robustus OX=1754192 PE=2 SV=1
10 AVO2_YEAST 22 47452 1 (1) 1 (1) 0.07 Target of rapamycin complex 2 subunit AVO2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=AVO2 PE=1 SV=1
11 JEN1_YEAST 21 69958 2 (1) 1 (1) 0.05 Carboxylic acid transporter protein homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=JEN1 PE=1 SV=1
12 MAD3_YEAST 21 59712 2 (1) 1 (1) 0.06 Spindle assembly checkpoint component MAD3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MAD3 PE=1 SV=1
13 PP2B1_YEAST 19 63532 1 (1) 1 (1) 0.05 Serine/threonine-protein phosphatase 2B catalytic subunit A1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CNA1 PE=1 SV=2
14 CUE2_YEAST 19 51013 3 (1) 2 (1) 0.06 Ubiquitin-binding protein CUE2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CUE2 PE=1 SV=1
15 TPT1_YEAST 17 26351 2 (1) 1 (1) 0.13 tRNA 2'-phosphotransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TPT1 PE=1 SV=1
16 VIP1_YEAST 15 130301 3 (1) 2 (1) 0.03 Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=VIP1 PE=1 SV=1

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