Mascot Search Results

User            : Monitor Test DB 0
Email           : 
Search title    : MS/MS Test Search
MS data file    : test_search.mgf
Database        : S-cerevisiae-S288C 20191206 (6886 sequences; 3133934 residues)
Timestamp       : 24 Jun 2025 at 21:20:02 GMT



Protein hits    : HSP60_YEAST Heat shock protein 60, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HSP60 PE=1 SV=1

Mascot Score Histogram

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 23 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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1.    HSP60_YEAST    Mass: 60999    Score: 45     Matches: 1(1)  Sequences: 1(1)
 Heat shock protein 60, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HSP60 PE=1 SV=1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   ppm  Miss Score Expect Rank Unique  Peptide
11   417.1822   832.3498   832.3828   -39.57 0  45  0.00026 1  U    K.APGFGDNR.K

Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   ppm  Miss Score Expect Rank Unique  Peptide
21   480.7447   959.4748   959.5586   -87.31 1  23  0.092 1       VTNMVKLR
16   456.7806   911.5467   911.5440   2.90 0  21  0.075 1       TAPAGAVVVK
34   714.8884   1427.7623   1427.8170   -38.31 1  14  0.66 1       RMLPSVILDSGIK
4   662.2756   661.2683   661.3282   -90.65 0  14  0.35 1       EAIDSK
14   442.2283   882.4421   882.5109   -78.03 1  12  0.81 1       IMNHKIK
26   608.3099   1214.6052   1214.6507   -37.43 0  12  0.94 1       ANGVDLVTALDK
27   617.2857   1232.5569   1232.6435   -70.26 0  12  0.86 1       VQSLALDCLSK
15   451.2499   900.4853   900.5644   -87.85 1  11  1.6 1       LSKEALIK
33   714.3649   1426.7153   1426.8217   -74.60 1  11  1 1       LSALCKTPPSLIK
6   673.3495   672.3422   672.3919   -73.84 0  8  3.5 1       AVITNR
31   672.8375   1343.6605   1343.6908   -22.52 0  7  2.5 1       TVEPVYQMHIK
35   714.8938   1427.7730   1427.8170   -30.78 1  7  2.9 1       RMLPSVILDSGIK
25   603.7720   1205.5294   1205.6114   -68.05 0  7  2.3 1       QLGMPLDFSAK
12   422.7433   843.4720   843.5429   -84.07 0  7  4 1       VALTIISK
9   747.3962   746.3889   746.4221   -44.50 1  6  7.1 1       SVVMRR
13   430.7328   859.4510   859.4651   -16.35 0  5  7.3 1       LLAETDAK
36   722.8849   1443.7552   1443.8119   -39.27 1  4  4.4 1       RMLPSVILDSGIK + Oxidation (M)
37   722.8934   1443.7722   1443.8119   -27.52 1  3  5.2 1       RMLPSVILDSGIK + Oxidation (M)
24   595.7855   1189.5565   1189.6706   -95.95 1  3  6.5 1       ELNEKIGFLK
44   949.5507   1897.0869   1897.0349   27.4 1  1  6.8 1       LLSWDSVFFIKNITSK
48   1019.5106   2037.0067   2036.9817   12.3 0  0  8.8 1       QQLEALSSVDFSHHFHR
1   498.2729   497.2656         
2   500.2560   499.2487         
3   575.5584   574.5511         
5   662.4172   661.4099         
7   711.3647   710.3574         
8   714.3725   713.3652         
10   747.4125   746.4052         
17   930.6831   929.6758         
18   930.7030   929.6957         
19   932.3644   931.3571         
20   933.4990   932.4917         
22   1101.5366   1100.5293         
23   1101.6217   1100.6144         
28   642.3526   1282.6906         
29   642.3536   1282.6926         
30   663.8379   1325.6612         
32   711.3707   1420.7269         
38   749.3840   1496.7534         
39   752.8643   1503.7141         
40   760.8461   1519.6777         
41   886.4059   1770.7972         
42   932.4608   1862.9071         
43   933.0038   1863.9930         
45   640.3281   1917.9625         
46   960.0327   1918.0509         
47   665.0096   1992.0069         
49   1020.9879   2039.9613         
50   1048.5615   2095.1085         
51   1057.0537   2112.0929         
52   1065.0399   2128.0653         
53   1065.0623   2128.1100         
54   1073.0477   2144.0809         
55   1099.0947   2196.1749         
56   1119.0452   2236.0758         
57   747.0361   2238.0864         
58   789.1062   2364.2968         
59   1183.1570   2364.2994         
60   789.1094   2364.3063         
61   828.1238   2481.3495         
62   828.1322   2481.3748         
63   832.7986   2495.3739         
64   854.0588   2559.1545         
65   1038.5031   3112.4873         
66   1113.8947   3338.6621         
67   1116.1775   3345.5106         

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin/P
Fixed modifications    : Carbamidomethyl (C)
Variable modifications : Oxidation (M)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 100 ppm
Fragment Mass Tolerance: ± 0.1 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Number of queries      : 67

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 22 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 2
Score greater than 21 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 3
Score greater than 13 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 4
Score greater than 18 indicates homology
Score greater than 22 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
 13.8      0.35    -90.65        K.EAIDSK.S
  5.1       2.6    -90.65        K.EADLSK.V
  4.5         3    -75.70        R.QNWSK.T
  2.0       5.3    -90.70        K.TDPTTK.E
  1.7       5.7    -90.65        R.IDSEAK.S
  1.2       6.3    -90.65        K.SLDEAK.E
  1.0       6.6    -35.65        K.SSDPEK.L
  1.0       6.6    -35.65        R.SSPEDK.R
  1.0       6.6    -35.65        R.SPSDEK.K
  0.6       7.3    -75.70        R.AYSHGK.V
Top scoring peptide matches to query 5
Score greater than 25 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 6
Score greater than 16 indicates homology
Score greater than 26 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
  7.6       3.5    -73.84        R.AVITNR.P
  3.1       9.9    -73.84        K.GILSQR.R
Top scoring peptide matches to query 7
Score greater than 23 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 8
Score greater than 23 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 9
Score greater than 18 indicates homology
Score greater than 27 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
  5.5       7.1    -44.50        R.SVVMRR.S
  4.9       8.2      34.3        R.VESPGMK.E
  4.4       9.2      14.2        K.SNNTRR.M
  3.6        11      29.2        R.ETGERR.L
  3.0        13      72.6        R.WEEGAR.T
  1.8        17    -78.17        K.MIRIAK.K
  1.7        17      4.26        K.IDGMRR.L
  1.6        17      34.3        K.PQDMLK.G
  0.9        21    -14.39        K.PTSLMAK.G
  0.4        23     -4.47        K.SVEERK.L
Top scoring peptide matches to query 10
Score greater than 27 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 11
Score greater than 22 indicates identity
Score    Expect      ppm   Hit  Protein      Peptide
 45.4   0.00026    -39.57    1   HSP60_YEAST K.APGFGDNR.K
  8.8       1.2    -96.19                    K.APVMIMR.D
  6.6         2    -43.60                    R.KMNDGPR.L
  4.8         3    -96.19                    K.APVMIMR.D
Top scoring peptide matches to query 12
Score greater than 19 indicates homology
Score greater than 25 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
  6.8         4    -84.07        K.VALTIISK.Q
  6.5       4.2    -11.10        R.REIDALK.K
  5.7       5.1    -11.14        K.GEVLGITR.F
  3.9       7.7    -11.12        K.QKNVVEK.D
  3.0       9.6    -11.14        VTDALAVR
  2.7        10      18.7        R.REQNVAK.E
  2.4        11    -24.43        R.TRLVEAR.V
  2.2        12      2.19        R.EIPSATVK.T
  1.9        12    -24.45        R.TVRNQVK.I
  1.8        13    -24.43        K.VQNRSLK.I
Top scoring peptide matches to query 13
Score greater than 16 indicates homology
Score greater than 26 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
  4.8       7.3    -16.35        K.LLAETDAK.Y
  3.1        11      32.8        R.PDWPFAK.T
  2.4        13      26.0        R.LEIGDDAK.L
  2.2        13    -17.91        R.EWTIRR.G
  1.1        17    -38.04        R.ICLDVLK.T
  1.1        17    -21.83        K.CLGRINK.W
  0.9        18      71.2        K.FFQMGSK.I
  0.5        20    -29.41        R.GATEKLNK.E
  0.4        20    -51.11        R.CVVKLNK.A
Top scoring peptide matches to query 14
Score greater than 19 indicates homology
Score greater than 24 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
 12.5      0.81    -78.03        R.IMNHKIK.T
 12.5      0.81    -89.97        K.IYLAYIK.A
 12.5      0.81    -15.71        R.LSHDNGIK.L
  5.8       3.8    -56.95        R.HTASGAVIK.G
  4.6         5    -11.35        K.IFSMLEK.F
  4.5       5.1    -89.99        R.YFLSLIK.G
  4.5       5.1    -89.97        K.YLAYLIK.I
  4.4       5.3    -56.95        K.SATAVAHVK.A
  3.5       6.4    -20.28        K.AIWGPDPK.E
  1.2        11    -69.90        K.LMFFVVK.K
Top scoring peptide matches to query 15
Score greater than 23 indicates homology
Score greater than 26 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
 11.2       1.6    -87.85        M.LSKEALIK.I
  9.9       2.1    -87.88        R.LLSQTVLK.D
  7.7       3.5    -87.86        K.KATLDLLK.D
  7.6       3.6      20.8        R.VTVDGSAPR.E
  7.6       3.6      28.9        R.ISDLYYK.S
  5.8       5.4      33.3        M.TVNLDPDK.V
  4.0       8.1    -59.93        K.LAKNNTLK.F
  4.0       8.1    -64.42        R.LIGWATIK.K
  3.4       9.5    -47.47        K.LGLTEQLK.G
  3.4       9.5    -87.85        R.KAIELSLK.E
Top scoring peptide matches to query 16
Score greater than 22 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
 20.8     0.075      2.90        K.TAPAGAVVVK.R
 14.8      0.29     -9.40        K.RLTPQAVK.I
  4.9       2.9      42.8        R.VLGIDGGPGK.E
  4.3       3.3      26.1        R.SYFLRVK.L
Top scoring peptide matches to query 17
Score greater than 13 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 18
Score greater than 13 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 19
Score greater than 18 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 20
Score greater than 25 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 21
Score greater than 25 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
 23.0     0.092    -87.31        M.VTNMVKLR.N
 12.2       1.1    -29.96        R.EANKETLR.Q
 11.3       1.3      3.74        K.ISDFPEPR.R
  6.6         4      19.7        R.EASTQEPAK.L
  5.9       4.7    -67.90        R.VTKEINTR.S
  5.0       5.7    -79.60        R.SLQRTSIR.E
  4.3       6.7     -3.76        K.VDTRNEAR.T
  4.3       6.7    -67.88        K.SINTELKR.E
  3.9       7.5    -67.88        R.SLTENKIR.I
  3.6         8    -83.78        R.FAKPIAASR.N
Top scoring peptide matches to query 22
Score greater than 24 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 23
Score greater than 25 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 24
Score greater than 14 indicates homology
Score greater than 24 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
  3.0       6.5    -95.95        K.ELNEKIGFLK.K
  1.5       9.2    -44.25        R.EGLQVLNFDR.T
Top scoring peptide matches to query 25
Score greater than 20 indicates homology
Score greater than 23 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
  7.1       2.3    -68.05        R.QLGMPLDFSAK.A
Top scoring peptide matches to query 26
Score greater than 17 indicates homology
Score greater than 24 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
 12.2      0.94    -37.43        K.ANGVDLVTALDK.Q
  4.2         6    -55.92        R.QRGNSISIGVGK.C
  4.0       6.3    -28.15        K.LLNEEEDLIK.P
  1.6        11    -46.65        K.SEARGNLLVEK.K
  1.0        12    -37.41        K.ELAPASSLATQK.L
  1.0        13    -58.11        K.DLKIEEIIDK.T
  0.9        13    -61.98        K.NAIKMAVELAR.K
Top scoring peptide matches to query 27
Score greater than 20 indicates homology
Score greater than 23 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
 11.6      0.86    -70.26        K.VQSLALDCLSK.L
  6.4       2.9    -99.75        K.KGESIAVLSAMK.M
  4.5       4.5    -11.25        K.GVGEMTPDQVGK.L
  0.5        11    -65.34        R.RGNPPNSQVHK.K
Top scoring peptide matches to query 28
Score greater than 24 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 29
Score greater than 24 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 30
Score greater than 23 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 31
Score greater than 16 indicates homology
Score greater than 24 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
  7.4       2.5    -22.52        K.TVEPVYQMHIK.T
  3.4       6.2    -14.00        K.PTNENSSARLQK.F
  0.8        11    -92.37        K.SLIEKGINVMIK.T
Top scoring peptide matches to query 32
Score greater than 23 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 33
Score greater than 15 indicates homology
Score greater than 23 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
 10.7         1    -74.60        K.LSALCKTPPSLIK.T
  2.1       7.4    -29.13        R.ISDVEVPHFTRK.S
  0.7        10    -38.87        K.SLSIGELSFLFSK.Y
Top scoring peptide matches to query 34
Score greater than 17 indicates homology
Score greater than 25 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
 13.8      0.66    -38.31        K.RMLPSVILDSGIK.E
  3.4       7.4     -2.61        R.KDIDTGFPPTPLK.R
  2.2       9.6    -46.17        -.MIRLAQQTQVLK.G
  0.4        15    -76.21        R.ISGLISKSIEILR.R
Top scoring peptide matches to query 35
Score greater than 17 indicates homology
Score greater than 24 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
  7.2       2.9    -30.78        K.RMLPSVILDSGIK.E
  3.8       6.4      4.91        R.KDIDTGFPPTPLK.R
  0.1        15    -68.69        R.ISGLISKSIEILR.R
Top scoring peptide matches to query 36
Score greater than 15 indicates homology
Score greater than 23 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
  4.1       4.4    -39.27        K.RMLPSVILDSGIK.E
  2.7       6.2    -47.05        -.MIRLAQQTQVLK.G
Top scoring peptide matches to query 37
Score greater than 16 indicates homology
Score greater than 23 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
  3.3       5.2    -27.52        K.RMLPSVILDSGIK.E
Top scoring peptide matches to query 38
Score greater than 23 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 39
Score greater than 23 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 40
Score greater than 23 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 41
Score greater than 23 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 42
Score greater than 23 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 43
Score greater than 22 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 44
Score greater than 13 indicates homology
Score greater than 21 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
  0.6       6.8      27.4        K.LLSWDSVFFIKNITSK.N
Top scoring peptide matches to query 45
Score greater than 22 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 46
Score greater than 22 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 47
Score greater than 22 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 48
Score greater than 13 indicates homology
Score greater than 22 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
  0.1       8.8      12.3        R.QQLEALSSVDFSHHFHR.T
Top scoring peptide matches to query 49
Score greater than 22 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 50
Score greater than 22 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 51
Score greater than 22 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 52
Score greater than 22 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 53
Score greater than 22 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 54
Score greater than 21 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 55
Score greater than 21 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 56
Score greater than 22 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 57
Score greater than 22 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 58
Score greater than 22 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 59
Score greater than 22 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 60
Score greater than 22 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 61
Score greater than 21 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 62
Score greater than 21 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 63
Score greater than 20 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 64
Score greater than 21 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 65
Score greater than 19 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 66
Score greater than 18 indicates identity
Score    Expect      ppm   Hit  Protein Peptide
Top scoring peptide matches to query 67
Score greater than 18 indicates identity
Score    Expect      ppm   Hit  Protein Peptide