MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:38:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 801–900 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
364 318.1922 317.1849
365 318.1923 317.1850
366 318.1935 317.1862
367 319.0491 318.0418
368 319.0492 318.0419
369 319.1159 318.1086
370 319.1162 318.1089
371 319.1163 318.1090
372 319.1164 318.1091
373 319.1164 318.1091
374 319.1166 318.1093
375 319.1167 318.1094
376 319.1167 318.1094
377 319.1528 318.1455
378 319.1530 318.1457
379 319.1531 318.1458
380 319.1535 318.1462
381 319.1539 318.1466
382 319.1539 318.1466
383 319.1541 318.1468
384 319.1542 318.1469
385 319.1544 318.1471
386 319.1547 318.1474
387 319.1548 318.1475
388 319.1549 318.1476
389 319.1550 318.1477
390 319.1552 318.1479
391 319.1552 318.1479
392 319.1678 318.1605
393 319.1738 318.1665
394 319.1895 318.1822
395 319.1901 318.1828
396 319.1903 318.1830
397 319.1908 318.1835
398 319.1908 318.1835
399 319.1909 318.1836
400 319.1909 318.1836
401 319.1909 318.1836
402 319.2263 318.2190
403 319.2271 318.2198
404 319.2271 318.2198
405 319.2275 318.2202
406 319.9941 318.9868
407 320.2076 319.2003
408 321.0668 320.0595
409 321.0945 320.0872
410 321.0953 320.0880
411 321.0958 320.0885
412 321.1310 320.1237
413 321.1310 320.1237
414 321.1310 320.1237
415 321.1315 320.1242
416 321.1317 320.1244
417 321.1318 320.1245
418 321.1319 320.1246
419 321.1319 320.1246
420 321.1320 320.1247
421 321.1322 320.1249
422 321.1323 320.1250
423 321.1326 320.1253
424 321.1327 320.1254
425 321.1328 320.1255
426 321.1332 320.1259
427 321.1332 320.1259
428 321.1686 320.1613
429 321.1692 320.1619
430 321.1692 320.1619
431 321.1692 320.1619
432 321.1693 320.1620
433 321.1694 320.1621
434 321.1697 320.1624
435 321.1698 320.1625
436 321.1698 320.1625
437 321.1699 320.1626
438 321.1700 320.1627
439 321.1700 320.1627
440 321.1701 320.1628
441 321.1703 320.1630
442 321.1703 320.1630
443 321.1703 320.1630
444 321.1703 320.1630
445 321.1705 320.1632
446 321.1706 320.1633
447 321.2056 320.1983
448 321.2066 320.1993
449 321.2067 320.1994
450 321.2070 320.1997
451 321.2415 320.2342
452 321.2421 320.2348
453 321.2430 320.2357
454 321.2432 320.2359
455 321.2609 320.2536
456 322.2234 321.2161
457 323.0480 322.0407
458 323.1100 322.1027
459 323.1107 322.1034
460 323.1107 322.1034
461 323.1108 322.1035
462 323.1111 322.1038
463 323.1115 322.1042
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