MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:38:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3801–3900 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4241 717.9855 3584.8911
4242 897.4761 3585.8753
4243 718.1827 3585.8771
4244 598.6536 3585.8779
4245 718.1838 3585.8826
4246 897.4786 3585.8853
4247 897.4786 3585.8853
4248 718.1844 3585.8856
4249 897.4794 3585.8885
4250 598.6555 3585.8893
4251 598.6555 3585.8893
4252 718.1853 3585.8901
4253 897.4799 3585.8905
4255 1197.6198 3589.8376
4256 721.1806 3600.8666
4259 901.2466 3600.9573
4260 903.4773 3609.8801
4263 905.9485 3619.7649
4264 725.3680 3621.8036
4265 906.4941 3621.9473
4266 725.5712 3622.8196
4267 725.5722 3622.8246
4268 906.7382 3622.9237
4270 725.9732 3624.8296
4271 907.4616 3625.8173
4272 728.3677 3636.8021
4273 728.5655 3637.7911
4274 728.7659 3638.7931
4275 728.7672 3638.7996
4276 729.3909 3641.9181
4277 729.3909 3641.9181
4279 729.7879 3643.9031
4280 911.9883 3643.9241
4281 914.7192 3654.8477
4282 914.9683 3655.8441
4283 732.9846 3659.8866
4285 735.5798 3672.8626
4287 919.7187 3674.8457
4288 920.7467 3678.9577
4289 617.8279 3700.9237
4290 617.8292 3700.9315
4291 529.7131 3700.9408
4292 529.7135 3700.9436
4293 529.7138 3700.9457
4294 741.3919 3701.9231
4295 529.8551 3701.9348
4296 529.8553 3701.9362
4298 741.3948 3701.9376
4299 463.7495 3701.9378
4300 741.3952 3701.9396
4301 529.8559 3701.9404
4303 529.8561 3701.9418
4305 741.3958 3701.9426
4307 463.7502 3701.9434
4308 463.7503 3701.9442
4309 617.9981 3701.9449
4310 741.3963 3701.9451
4312 617.9983 3701.9461
4314 529.8569 3701.9474
4315 617.9987 3701.9485
4318 529.8571 3701.9488
4319 463.8753 3702.9442
4321 934.2240 3732.8669
4322 934.4666 3733.8373
4324 938.4525 3749.7809
4325 938.7637 3751.0257
4326 938.7661 3751.0353
4327 943.5016 3769.9773
4328 947.7557 3786.9937
4329 950.7405 3798.9329
4330 766.7990 3828.9586
4332 958.5194 3830.0485
4334 961.9958 3843.9541
4335 961.9987 3843.9657
4336 967.9880 3867.9229
4338 777.5902 3882.9146
4339 973.5004 3889.9725
4340 973.5020 3889.9789
4341 973.5023 3889.9801
4342 975.4766 3897.8773
4343 975.5242 3898.0677
4344 786.4139 3927.0331
4345 786.4162 3927.0446
4346 786.4176 3927.0516
4347 990.7881 3959.1233
4348 661.0257 3960.1105
4349 661.0258 3960.1111
4350 1001.0185 4000.0449
4351 1002.2587 4005.0057
4352 1002.2842 4005.1077
4353 1002.4881 4005.9233
4354 805.6258 4023.0926
4355 805.8260 4024.0936
4356 1007.0352 4024.1117
4357 674.3613 4040.1241
4358 674.3615 4040.1253
4359 809.2300 4041.1136
4360 809.2308 4041.1176
4361 674.5271 4041.1189
4362 809.2312 4041.1196
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