MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:38:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3501–3600 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3852 894.7747 2681.3023
3853 672.3414 2685.3365
3854 896.4647 2686.3723
3855 897.7573 2690.2501
3856 897.8036 2690.3890
3857 539.2583 2691.2551
3858 673.8221 2691.2593
3859 539.2597 2691.2621
3860 673.8229 2691.2625
3861 898.1172 2691.3298
3864 898.4457 2692.3153
3865 898.7852 2693.3338
3866 898.8434 2693.5084
3867 674.5481 2694.1633
3868 903.0776 2706.2110
3869 677.5623 2706.2201
3870 542.2518 2706.2226
3871 677.5640 2706.2269
3872 903.4786 2707.4140
3873 904.4512 2710.3318
3874 904.4517 2710.3333
3875 678.5923 2710.3401
3876 678.8425 2711.3409
3877 543.6995 2713.4611
3878 679.3728 2713.4621
3879 543.6998 2713.4626
3880 679.3740 2713.4669
3881 680.0946 2716.3493
3883 908.8045 2723.3917
3885 910.1264 2727.3574
3886 683.5712 2730.2557
3900 912.4580 2734.3522
3902 912.4672 2734.3798
3903 685.3639 2737.4265
3905 549.0635 2740.2811
3906 916.4700 2746.3882
3907 916.8113 2747.4121
3908 916.8118 2747.4136
3909 917.8386 2750.4940
3910 917.8412 2750.5018
3911 917.8432 2750.5078
3912 688.6352 2750.5117
3913 688.6354 2750.5125
3914 917.8452 2750.5138
3915 919.4332 2755.2778
3916 690.7896 2759.1293
3917 552.8336 2759.1316
3918 922.1294 2763.3664
3919 922.1323 2763.3751
3920 922.1374 2763.3904
3921 692.3340 2765.3069
3922 922.8355 2765.4847
3923 693.0964 2768.3565
3927 924.4412 2770.3018
3928 925.1754 2772.5044
3930 925.8417 2774.5033
3931 926.1693 2775.4861
3932 926.1694 2775.4864
3933 926.4413 2776.3021
3934 926.4501 2776.3285
3935 695.3798 2777.4901
3936 695.8721 2779.4593
3937 927.7698 2780.2876
3940 929.1132 2784.3178
3941 697.0880 2784.3229
3942 929.4605 2785.3597
3943 929.5016 2785.4830
3944 929.5040 2785.4902
3945 930.1168 2787.3286
3948 698.3375 2789.3209
3950 558.8979 2789.4531
3951 698.3758 2789.4741
3952 931.1881 2790.5425
3953 932.4447 2794.3123
3954 932.4451 2794.3135
3955 932.4468 2794.3186
3957 700.8441 2799.3473
3958 701.3379 2801.3225
3960 934.8003 2801.3791
3961 935.1248 2802.3526
3962 935.1254 2802.3544
3963 935.1262 2802.3568
3964 935.1271 2802.3595
3965 935.4565 2803.3477
3967 702.8884 2807.5245
3968 703.1332 2808.5037
3969 703.8689 2811.4465
3970 938.5045 2812.4917
3971 704.3475 2813.3609
3972 704.8654 2815.4325
3973 941.1358 2820.3856
3974 941.4783 2821.4131
3975 942.1219 2823.3439
3976 942.1249 2823.3529
3977 942.8165 2825.4277
3978 943.8599 2828.5579
3979 945.5022 2833.4848
3980 946.1495 2835.4267
3982 948.5438 2842.6096
3983 949.4470 2845.3192
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