MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:38:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3401–3500 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3684 808.4586 2422.3540
3692 808.7812 2423.3218
3693 812.4156 2434.2250
3694 812.4560 2434.3462
3695 813.7659 2438.2759
3696 813.7826 2438.3260
3697 814.0947 2439.2623
3707 613.5824 2450.3005
3708 818.7841 2453.3305
3709 819.7729 2456.2969
3711 820.1387 2457.3943
3712 820.4409 2458.3009
3713 616.3143 2461.2281
3716 822.1049 2463.2929
3717 822.1068 2463.2986
3718 822.7716 2465.2930
3720 823.1255 2466.3547
3721 823.4412 2467.3018
3722 823.4489 2467.3249
3725 620.0554 2476.1925
3726 827.1340 2478.3802
3728 827.4222 2479.2448
3732 827.4664 2479.3774
3733 828.1046 2481.2920
3734 829.1583 2484.4531
3735 829.4243 2485.2511
3739 624.5630 2494.2229
3740 832.4349 2494.2829
3742 833.8074 2498.4004
3743 834.4060 2500.1962
3744 835.0849 2502.2329
3745 835.4437 2503.3093
3746 835.7896 2504.3470
3747 836.7562 2507.2468
3749 837.4484 2509.3234
3752 839.1412 2514.4018
3753 839.4708 2515.3906
3754 839.7570 2516.2492
3756 840.0704 2517.1894
3758 841.3969 2521.1689
3759 844.4127 2530.2163
3761 634.0482 2532.1637
3765 848.8040 2543.3902
3766 637.1064 2544.3965
3767 637.1083 2544.4041
3768 637.5726 2546.2613
3769 637.7860 2547.1149
3770 850.4363 2548.2871
3771 851.1148 2550.3226
3772 851.1411 2550.4015
3773 851.7773 2552.3101
3775 852.4305 2554.2697
3776 852.4308 2554.2706
3777 639.8251 2555.2713
3780 856.4405 2566.2997
3781 857.4290 2569.2652
3782 857.4600 2569.3582
3783 643.5671 2570.2393
3784 857.7543 2570.2411
3786 858.1456 2571.4150
3789 861.4423 2581.3051
3790 861.4501 2581.3285
3791 861.4515 2581.3327
3792 862.4373 2584.2901
3793 862.4398 2584.2976
3795 647.3103 2585.2121
3797 864.4883 2590.4431
3799 650.8545 2599.3889
3800 867.7982 2600.3728
3801 651.1035 2600.3849
3802 869.1319 2604.3739
3803 653.0543 2608.1881
3804 873.0826 2616.2260
3805 873.0860 2616.2362
3806 873.4180 2617.2322
3807 656.7960 2623.1549
3808 876.7466 2627.2180
3810 877.4631 2629.3675
3811 878.7864 2633.3374
3813 660.0800 2636.2909
3814 660.0801 2636.2913
3818 883.1375 2646.3907
3819 883.4703 2647.3891
3820 883.4727 2647.3963
3833 885.4446 2653.3120
3835 664.3366 2653.3173
3838 885.4632 2653.3678
3839 886.4222 2656.2448
3840 889.1021 2664.2845
3841 890.1556 2667.4450
3842 890.4393 2668.2961
3843 890.4395 2668.2967
3844 668.3303 2669.2921
3845 668.3329 2669.3025
3846 668.3333 2669.3041
3847 892.7681 2675.2825
3848 536.2605 2676.2661
3849 893.1315 2676.3727
3850 670.3251 2677.2713
3851 670.5556 2678.1933
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