MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:38:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3301–3400 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3534 562.2529 2244.9825
3535 1124.1224 2246.2302
3536 750.0874 2247.2404
3537 750.3687 2248.0843
3540 751.3868 2251.1386
3547 755.6774 2264.0104
3548 758.3843 2272.1311
3549 759.3491 2275.0255
3553 761.6774 2282.0104
3554 761.6813 2282.0221
3555 1142.0201 2282.0256
3556 761.6847 2282.0323
3561 573.4844 2289.9085
3563 1147.5800 2293.1454
3565 765.4184 2293.2334
3566 765.4187 2293.2343
3567 765.4204 2293.2394
3568 765.4207 2293.2403
3569 765.4207 2293.2403
3570 765.4210 2293.2412
3571 765.4222 2293.2448
3572 765.4229 2293.2469
3573 765.4238 2293.2496
3575 766.6802 2297.0188
3576 766.6838 2297.0296
3577 575.4774 2297.8805
3579 767.0870 2298.2392
3580 767.4055 2299.1947
3584 1152.0658 2302.1170
3586 577.2552 2304.9917
3587 769.7018 2306.0836
3588 769.7023 2306.0851
3589 1154.0500 2306.0854
3590 770.3578 2308.0516
3592 1157.0646 2312.1146
3593 771.7132 2312.1178
3595 773.6718 2317.9936
3597 580.7492 2318.9677
3598 580.7564 2318.9965
3599 774.0513 2319.1321
3600 774.3308 2319.9706
3605 581.3098 2321.2101
3606 775.4230 2323.2472
3607 776.3624 2326.0654
3608 776.4097 2326.2073
3609 1165.0632 2328.1118
3611 778.9943 2333.9611
3612 584.4982 2333.9637
3613 779.0837 2334.2293
3614 779.3195 2334.9367
3615 779.3989 2335.1749
3616 584.9935 2335.9449
3617 779.7275 2336.1607
3618 1169.5842 2337.1538
3619 780.6932 2339.0578
3620 780.8002 2339.3788
3622 781.0804 2340.2194
3623 586.3008 2341.1741
3624 782.0505 2343.1297
3625 782.0543 2343.1411
3626 782.3508 2344.0306
3627 587.0162 2344.0357
3633 784.0274 2349.0604
3634 784.1240 2349.3502
3635 784.3904 2350.1494
3638 785.0673 2352.1801
3639 786.4240 2356.2502
3640 590.5322 2358.0997
3641 787.3735 2359.0987
3642 787.7517 2360.2333
3644 788.4197 2362.2373
3645 789.6863 2366.0371
3647 592.8086 2367.2053
3649 790.7856 2369.3350
3651 593.8407 2371.3337
3652 593.8413 2371.3361
3653 593.8416 2371.3373
3655 475.4740 2372.3336
3657 792.3600 2374.0582
3658 594.5253 2374.0721
3659 794.4095 2380.2067
3660 596.8019 2383.1785
3662 795.7166 2384.1280
3663 477.8430 2384.1786
3664 795.7335 2384.1787
3665 795.7377 2384.1913
3666 796.9561 2387.8465
3667 597.9704 2387.8525
3668 797.4327 2389.2763
3669 797.4338 2389.2796
3670 799.1267 2394.3583
3671 800.7226 2399.1460
3672 600.7939 2399.1465
3675 802.0925 2403.2557
3676 804.7884 2411.3434
3677 805.1274 2412.3604
3678 805.3744 2413.1014
3679 805.3779 2413.1119
3680 806.1050 2415.2932
3682 808.4493 2422.3261
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