MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:38:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3001–3100 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3076 780.9238 1559.8330
3077 780.9260 1559.8374
3078 780.9288 1559.8430
3079 780.9309 1559.8472
3082 783.3895 1564.7644
3086 527.9202 1580.7388
3087 527.9417 1580.8033
3088 791.8842 1581.7538
3090 396.4549 1581.7905
3094 530.3111 1587.9115
3096 796.9376 1591.8606
3098 798.9164 1595.8182
3100 533.5928 1597.7566
3112 806.0054 1609.9962
3113 807.4658 1612.9170
3114 538.6466 1612.9180
3115 538.6471 1612.9195
3116 807.9169 1613.8192
3117 807.9191 1613.8236
3118 807.9203 1613.8260
3119 809.4403 1616.8660
3120 809.9105 1617.8064
3121 540.6002 1618.7788
3122 810.4177 1618.8208
3123 810.4178 1618.8210
3124 810.4202 1618.8258
3125 540.9382 1619.7928
3127 810.9083 1619.8020
3131 409.1843 1632.7081
3132 818.8915 1635.7684
3133 409.9495 1635.7689
3134 546.2654 1635.7744
3136 546.3090 1635.9052
3137 546.5999 1636.7779
3138 820.4320 1638.8494
3139 821.4562 1640.8978
3142 825.9255 1649.8364
3143 825.9296 1649.8446
3144 826.3744 1650.7342
3145 551.2910 1650.8512
3146 413.9422 1651.7397
3148 551.5895 1651.7467
3149 828.9285 1655.8424
3156 833.9308 1665.8470
3157 556.6240 1666.8502
3158 834.4439 1666.8732
3159 556.6317 1666.8733
3160 834.4456 1666.8766
3161 834.4496 1666.8846
3162 834.9933 1667.9720
3163 556.9980 1667.9722
3164 834.9948 1667.9750
3165 556.9994 1667.9764
3167 557.0001 1667.9785
3168 835.4941 1668.9736
3169 835.4955 1668.9764
3170 557.3336 1668.9790
3171 835.4978 1668.9810
3173 836.9415 1671.8684
3174 837.4624 1672.9102
3175 558.6475 1672.9207
3176 837.4724 1672.9302
3178 838.4520 1674.8894
3179 838.4540 1674.8934
3182 838.9561 1675.8976
3186 421.2198 1680.8501
3187 561.2932 1680.8578
3188 841.9435 1681.8724
3190 842.4252 1682.8358
3196 842.9343 1683.8540
3199 562.3138 1683.9196
3200 564.2375 1689.6907
3201 564.9125 1691.7157
3202 565.9849 1694.9329
3203 849.4238 1696.8330
3205 425.2168 1696.8381
3213 427.2121 1704.8193
3214 570.6678 1708.9816
3216 856.4435 1710.8724
3217 857.4086 1712.8026
3218 429.2084 1712.8045
3222 572.9861 1715.9365
3223 859.4631 1716.9116
3224 859.4690 1716.9234
3225 573.3223 1716.9451
3226 860.4210 1718.8274
3230 575.2247 1722.6523
3231 862.4243 1722.8340
3232 863.9896 1725.9646
3233 576.3303 1725.9691
3234 866.0077 1730.0008
3236 579.6166 1735.8280
3237 580.6379 1738.8919
3238 580.6382 1738.8928
3240 870.9755 1739.9364
3241 580.9877 1739.9413
3243 581.3312 1740.9718
3244 581.6425 1741.9057
3245 871.9610 1741.9074
3247 581.9755 1742.9047
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