MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:38:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2901–3000 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2864 451.5419 1351.6039
2865 452.2350 1353.6832
2866 452.2355 1353.6847
2867 455.2718 1362.7936
2868 455.5839 1363.7299
2869 455.9341 1364.7805
2870 455.9447 1364.8123
2872 690.3713 1378.7280
2873 461.2744 1380.8014
2874 691.9263 1381.8380
2875 691.9285 1381.8424
2898 698.8464 1395.6782
2900 466.5523 1396.6351
2901 700.3607 1398.7068
2902 701.8718 1401.7290
2911 471.2682 1410.7828
2913 471.2684 1410.7834
2914 471.2690 1410.7852
2915 472.5575 1414.6507
2920 474.6093 1420.8061
2922 712.4040 1422.7934
2927 476.9387 1427.7943
2932 478.5833 1432.7281
2933 717.3720 1432.7294
2934 359.1904 1432.7325
2935 478.5850 1432.7332
2936 717.8735 1433.7324
2937 721.3662 1440.7178
2938 481.2648 1440.7726
2939 481.2651 1440.7735
2940 721.8594 1441.7042
2941 481.9349 1442.7829
2942 481.9354 1442.7844
2943 481.9358 1442.7856
2944 722.4003 1442.7860
2945 481.9372 1442.7898
2948 723.3913 1444.7680
2949 362.9306 1447.6933
2950 724.8542 1447.6938
2951 483.5735 1447.6987
2953 484.9422 1451.8048
2954 484.9447 1451.8123
2955 484.9447 1451.8123
2956 726.9148 1451.8150
2960 728.4329 1454.8512
2961 487.2656 1458.7750
2962 487.2691 1458.7855
2963 730.8849 1459.7552
2964 487.5946 1459.7620
2965 730.9256 1459.8366
2966 367.1913 1464.7361
2967 489.2535 1464.7387
2968 489.9464 1466.8174
2969 489.9470 1466.8192
2970 734.9124 1467.8102
2973 494.2430 1479.7072
2974 741.9341 1481.8536
2975 742.3813 1482.7480
2976 742.9081 1483.8016
2977 495.6123 1483.8151
2978 495.6126 1483.8160
2985 743.4225 1484.8304
2999 748.4187 1494.8228
3000 749.3622 1496.7098
3001 749.8861 1497.7576
3012 752.3911 1502.7676
3014 752.8937 1503.7728
3015 502.2659 1503.7759
3016 377.7057 1506.7937
3021 504.6241 1510.8505
3028 759.3820 1516.7494
3029 759.3823 1516.7500
3032 305.5615 1522.7711
3033 381.7010 1522.7749
3034 508.5990 1522.7752
3035 381.7016 1522.7773
3037 381.7019 1522.7785
3038 508.9353 1523.7841
3041 509.9461 1526.8165
3042 764.9100 1527.8054
3043 764.9177 1527.8208
3044 764.9276 1527.8406
3045 765.4284 1528.8422
3046 510.6243 1528.8511
3047 765.4333 1528.8520
3051 767.3857 1532.7568
3052 513.5788 1537.7146
3053 385.4361 1537.7153
3054 769.8751 1537.7356
3055 513.5876 1537.7410
3056 385.4431 1537.7433
3057 513.5887 1537.7443
3060 514.9572 1541.8498
3061 514.9584 1541.8534
3062 772.4322 1542.8498
3063 772.8873 1543.7600
3070 775.3810 1548.7474
3071 775.4075 1548.8004
3073 775.9098 1549.8050
3074 518.8975 1553.6707
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