MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:38:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2401–2500 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1965 437.2173 436.2100
1966 437.2175 436.2102
1967 437.2175 436.2102
1968 437.2179 436.2106
1969 437.2185 436.2112
1970 437.2188 436.2115
1971 439.1959 438.1886
1972 439.1972 438.1899
1973 439.2152 438.2079
1974 439.2156 438.2083
1975 439.2157 438.2084
1976 439.2158 438.2085
1977 439.2161 438.2088
1978 439.2162 438.2089
1979 439.2163 438.2090
1980 439.2165 438.2092
1981 439.2165 438.2092
1982 439.2165 438.2092
1983 439.2166 438.2093
1984 439.2308 438.2235
1985 439.2319 438.2246
1986 439.2328 438.2255
1987 439.2335 438.2262
1988 439.2339 438.2266
1989 440.2615 439.2542
1990 441.1443 440.1370
1991 441.2308 440.2235
1992 441.2323 440.2250
1993 441.2324 440.2251
1994 441.2326 440.2253
1995 442.0942 441.0869
1996 442.1140 441.1067
1997 443.0430 442.0357
1998 443.1598 442.1525
1999 443.1601 442.1528
2000 443.2464 442.2391
2001 443.2473 442.2400
2002 443.2475 442.2402
2003 443.2478 442.2405
2004 443.2481 442.2408
2005 443.2489 442.2416
2006 444.0720 443.0647
2007 444.1075 443.1002
2008 444.1095 443.1022
2009 446.2963 445.2890
2010 448.3120 447.3047
2011 448.3127 447.3054
2012 448.3127 447.3054
2013 448.3128 447.3055
2014 448.3132 447.3059
2015 448.3139 447.3066
2016 449.1517 448.1444
2017 449.2194 448.2121
2018 451.1971 450.1898
2019 452.0273 451.0200
2020 453.2115 452.2042
2021 453.2133 452.2060
2022 453.2134 452.2061
2023 453.2134 452.2061
2024 453.3454 452.3381
2025 453.7879 452.7806
2026 453.7881 452.7808
2027 453.7892 452.7819
2028 455.0656 454.0583
2029 455.1612 454.1539
2030 457.1391 456.1318
2031 457.1392 456.1319
2032 457.2257 456.2184
2033 457.2805 456.2732
2034 458.0890 457.0817
2035 458.2976 457.2903
2036 458.3106 457.3033
2037 460.2783 459.2710
2039 461.2487 460.2414
2040 461.2560 460.2487
2041 461.2571 460.2498
2043 461.2767 460.2694
2044 464.0283 463.0210
2045 465.8091 464.8018
2046 465.8094 464.8021
2047 466.0434 465.0361
2048 466.0918 465.0845
2049 466.3229 465.3156
2050 467.2290 466.2217
2051 467.8021 466.7948
2052 467.8025 466.7952
2053 467.8030 466.7957
2054 467.8033 466.7960
2055 467.8033 466.7960
2056 467.8035 466.7962
2057 467.8039 466.7966
2058 467.8040 466.7967
2059 467.8044 466.7971
2060 467.8045 466.7972
2061 467.8046 466.7973
2062 467.8052 466.7979
2063 467.8057 466.7984
2064 468.2843 467.2770
2065 469.1768 468.1695
2066 469.2422 468.2349
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